Aster Bio Environmental Genomics is 16S DNA microbial census for wastewater and soil samples. Benefits & applications of data are given.

1. Environmental Genomics™
Using Advanced DNA Sequencing to Conduct a Microbial Census
Aster Bio
www.asterbio.com
info@asterbio.com
713.734.0082

2. Environmental Genomics™ Testing – A True Microbial Census
• DNA-based test using genetic material
from the 16S rDNA region in bacteria
• Identifies what microbes are growing in
the sample
• Gives a relative frequency of each
microbial form
• Includes cultures that are difficult or
impossible to grow on conventional
plates
© 2003 – 2016, Aster Bio, Inc. All Rights Reserved 1

3. How Does Environmental Genomics
Relate to Other Monitoring Methods?
© 2003 – 2016, Aster Bio, Inc. All Rights Reserved 2
1st Generation
• MLSS
• SV30
• Nutrient residuals
2st Generation
• ATP
• Respirometer (OUR)
• Microscopic Exam
3rd Generation
• 16S Metagenomics
• FISH (DNA Markers)
Better Direct Information on Biomass

4. Benefits from Environmental Genomics™ Testing
© 2003 – 2016, Aster Bio, Inc. All Rights Reserved 3
• Identification of microbes associated with bulking or high EPS production
• Evaluate how any system change impacts the biomass
• Changes in MCRT
• Temperature
• D.O. & mixing
• Investigate Biological Nutrient Removal (BNR) efficiency
• Nutrient formulation changes
• New waste streams
• Impact of bioadditives
• Formulation of custom bioaugmentation products

5. Example Applications of Environmental Genomics™ Testing
© 2003 – 2016, Aster Bio, Inc. All Rights Reserved 4

6. Identify Sources of Filamentous Bulking
© 2003 – 2016, Aster Bio, Inc. All Rights Reserved 5
An industrial facility operating an extended aeration
activated sludge unit (ASU) was experiencing sustained
filamentous bulking issues. Even with sufficient D.O.
according to D.O. meter checks, filaments were causing
bulking and excess secondary polymer use.
Previously, the facility had used outside microscopic exam
to evaluate the sludge and filaments present. Aster Bio
wanted to test the Environmental Genomics platform to
see if 16S could identify the filaments and other microbial
forms causing bulking.
Testing found the system had significant concentrations of
Hyphomicrobium sp. The subsequent investigation found
the bulking was response to high levels of methanol in
the influent, not a previously suspected nutrient or
dissolved oxygen sufficiency.

7. Bioremediation – Creating a Custom Consortium
© 2003 – 2016, Aster Bio, Inc. All Rights Reserved 6
In China, a former industrial chemical site had
discharged wastewater in a lagoon system
without any advanced biological treatment. The
sludge buildup contained many recalcitrant
compounds including pesticides, herbicides,
PAHs, and chlorinated compounds. Initial
attempts at bioremediation including adding
bioaugmentation cultures had not succeeded in
removing the criteria pollutants.
Aster Bio worked with local remediation
specialists to characterize the waste and used
Environmental Genomics™ technology to
identify cultures capable of treating the sludge.
Using data from the testing, Aster Bio was able
to create a microbial consortium that would
initiate the bioremediation process and speed
up site closure.

8. About Aster Bio
We put Mother Nature to work Our bugs are better
Microorganisms are Nature’s recyclers. At Aster Bio, we put Earth-friendly
microorganisms to work to treat biological waste and reduce pollution and toxic
compounds in wastewater, groundwater and soil.
Aster Bio is the only biological solutions company to offer leading-edge
multigenomic screening technologies combined with time-tested environmental
microbiology techniques to create customized biochemical products for better
results.
The Aster Bio Difference We save our customers’ money
Aster Bio scientists are experts in microbial ecology, biochemistry and waste
treatment. We’ve developed a leading-edge biomass management approach,
including our Environmental Genomics testing, to make biological treatments
work better. We use custom bioaugmentation* formulations, micronutrients
and process monitoring programs to help our customers meet their waste
treatment goals.
Aster Bio can maximize a customer’s treatment system’s performance while
reducing chemical, utility and capital costs. We analyze the biological treatment
system with a biomass evaluation/system audit and our exclusive Environmental
Genomics DNA testing to develop a customized, effective bioaugmentation
solution.
Micro solutions for big industry challenges A spirit of continuous innovation
We formulate customized solutions to optimize wastewater biomass in
municipal, pulp and paper, petrochemical, food processing and organic chemical
wastewater treatment systems. Our formulations are used to enhance
bioremediation of soils and waters contaminated by hydrocarbons and other
recalcitrant chemicals.
While we make great products and have strong application technologies, Aster
Bio strives to find new, better biochemical processes, organisms, and application
technologies that improve on existing products and extend natural biological
technologies into applications currently served only by chemical treatments.
© 2003 – 2016, Aster Bio, Inc. All Rights Reserved 7
* bioaugmentation: adding cultured microorganisms into water or soil for the purpose of degrading contaminants