Electrophoretic Characterization of Plasminogen Activator Proposal
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The document summarizes the use of electrophoretic techniques to characterize plasminogen activator isolated from human cell culture broth. Specifically, SDS-PAGE and native gel electrophoresis will be used to determine the molecular size, purity, and activity of plasminogen activator from HEK-293 cells and JURKAT cells. The Laemmli system will be applied using stacking and resolving gels to separate protein samples based on charge and size.
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Electrophoresis presentation
Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids based on their size and charge. It works by applying an electric current to move these molecules through a medium like a gel or paper. The document discusses different types of electrophoresis like gel electrophoresis, paper electrophoresis, and isolectric focusing. It also explains how various factors like the molecule's charge, size, and shape affect its movement during electrophoresis.
Electrophoresis and its types and its importance in Genetic engineering
This document discusses electrophoresis, which is a technique used to separate biomolecules like DNA, RNA, and proteins based on their size and charge. It moves through two common media - agarose gel and polyacrylamide gel. Agarose gel is used for larger molecules while polyacrylamide gel provides better resolution for smaller molecules. The document explains the principles, factors affecting separation, different types like agarose gel electrophoresis and polyacrylamide gel electrophoresis. It also discusses the requirements, steps involved and applications of agarose and polyacrylamide gel electrophoresis.
Electrophoresis
This document discusses various types of electrophoresis techniques. It begins by explaining that electrophoresis involves charged molecules like proteins and nucleic acids migrating in response to an electrical field. It then discusses different gel types like agarose and polyacrylamide gels that are used as supporting matrices. It also covers techniques like SDS-PAGE, isoelectric focusing, two-dimensional gel electrophoresis, and capillary electrophoresis. The document provides details on how each technique separates molecules based on properties like size, charge, and isoelectric point.
Isolation of photochrome transmembrane protein
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Characterization of nucleic acids and protein by electrophoresis
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