Methylation is a minor pathway of metabolizing foreign substances where methyl groups are added via N-, O-, S- or arsenic-methylation, using S-adenosylmethionine as a cofactor. Methylation reactions metabolize small endogenous compounds as well as macromolecules and some drugs. Acetylation involves transferring acetyl groups from acetyl coenzyme A to aromatic amines or hydrazines, converting them to aromatic amides or hydrazides. N-acetylation is a major pathway for detoxifying arylamine compounds in animals and humans. O- and N,O- acetylations can activate compounds through N-hydroxylation, forming unstable arylnitrenium ions that bind