High density chitosan membranes were produced via a novel manufacturing process involving casting, freezing, neutralizing, and applying compression and dehydration. The resulting membranes have densities as high as 1.6 g/cm3 and superior physical properties compared to commercial collagen membranes. They exhibit biocompatibility and are resorbable in vivo, demonstrating potential as surgical barrier membranes, tissue scaffolds, wound dressings, or drug delivery devices.
Wound healing is an important concern these days and there are lots of various medications for it.
One of the most effective methods is Applying Acellular Matrices to the wound bed.
In this presentation, It was tried to explain the application of these products as well as the complications of usage.
I wish it be useful for anyone.
It would be appreciated if you let me know your comments on my presentation.
sincerely,
S. Mohammad Zargar
Contact me: s.mohammad.zargar@gmail.com
This document describes the development of a high content screening assay to evaluate the effects of degradation products from gelatin-based biomaterials on osteoclast differentiation. The assay uses the murine monocytic cell line RAW 264.7, which can differentiate into osteoclasts. Degradation products from six different gelatin-lysine diisocyanate hydrogel compositions were tested in the assay. The degradation products were found to inhibit the formation of multinucleated osteoclasts, suggesting they may support bone regeneration by suppressing bone resorption. The assay provides a way to quantitatively assess biomaterial effects on osteoclast fusion and differentiation in a high throughput manner.
This document reviews the investigation of mechanical properties of chitosan-based films. It discusses how chitosan films are typically produced using either a solvent casting (wet) method or thermo-mechanical (dry) method. The solvent casting method, where a chitosan-acid solution is cast and dried, is most common for producing films for food applications due to advantages like transparency and flexibility. The review summarizes several studies that have investigated the mechanical properties like tensile strength of chitosan films and composites with other materials, finding properties can be improved through the addition of plasticizers or nanomaterials.
This document summarizes new wound management techniques and products. It discusses how selecting appropriate wound care can positively influence repair by understanding repair mechanisms. During the inflammatory phase, debridement and dressings can accelerate healing by enhancing inflammation. Products like Solcoseryl and hydrogels can stimulate early repair but may prolong inflammation later. Sugar, honey, and ketanserin can activate macrophages and improve inflammation. Chronic wounds get "stuck" in inflammation; treatments aim to identify and remove obstacles and create a healing environment. Later, growth factors and dressings that release them can enhance proliferation while limiting scarring.
In Vitro Cell Tests for Functional FoodInstitut Kurz
The relationship between the food we eat and our health is
clear. In the constant search for healthier foods rich in
bioactive compounds that promote health and healthy
ageing, a wide variety of functional foods have appeared on
the market.
To know the real function of these functional foods
in our body, it is necessary to carry out different types of in
vitro cell tests.
Institut Kurz specializes in conducting in vitro cell tests for functional foods.
Contact us for more information:
info@institut-kurz.com
https://www.institut-kurz.com/
Objective: In order to reduce complications accompanied with dental implant restoration, this study strives to prepare a novel sealant and lubricant that can be used in dental implant systems as well as to evaluate its characteristics.
Study Design: Chitosan (CS), β-glycerophosphate pentahydrate (β-GP), and nano silver (nAg) were used to prepare thermosensitive hydrogel. According to the different volume ratios of CS to β-GP, 3 experimental groups were established, namely 16/4, 13/7, and 10/10 groups. Their morphology, composition, and chemical properties were analyzed via SEM, EDS, and FTIR. In addition, the effect of the hydrogel on the stability of dental implant-abutment connection was investigated by removal torque test combined with dynamic cyclic loading experiment. The maximum fracture load was measured under different lubricating conditions by electronic universal testing machine. The cytotoxicity and in vitro antibacterial effect of the hydrogel were examined respectively by CCK-8 test and the spread plate method.
Results: The CS/β-GP/nAg thermosensitive hydro-gel was successfully prepared in this study, which was found to be a porous structure through SEM. The removal torque test and the dynamic cyclic loading experiment showed that the removal torque of the experimental group was greater than that of the control group. Furthermore, the single load-to-fracture test indicated that the 16/4 group had the greatest maximum bearing load. The in vitro cytotoxicity test using rat bone marrow stromal cells (rBMSCs) and human gingival fibroblast cells (hGFCs) showed no cytotoxicity in all 3 groups. The 3 experimental groups had obvious antibacterial effects against E. coli, S. aureus, and P. gingivalis.
Conclusion: A nontoxic antibacterial CS/β-GP/nAg thermosensitive hydrogel for lubricating purpose was successfully fabricated. When the volume ratio of CS to β-GP was 16/4, this thermosensitive hydrogel demonstrated better sealing and lubricating abilities and had a positive influence on the reliability of dental implant-abutment connection.
Keywords: abutment, dental implant, dental implant restoration, dental sealant, lubrication, thermosensitive hydrogel
Aligned and random nanofibrous nanocomposite scaffolds for bone tissue engine...Nanomedicine Journal (NMJ)
Abstract
Aligned and random nanocomposite nanofibrous scaffolds were electrospun from polycaprolactone (PCL), poly (vinyl alcohol) (PVA) and hydroxyapatite nanoparticles (nHA). The morphology and mechanical characteristics of the nanofibers were evaluated using scanning electron microscopy and tensile testing, respectively. Scanning electron microscopy revealed fibers with an average diameter of 123 ± 32 nm and 339 ± 107 nm for aligned and random nanofibers, respectively. The mechanical data indicated the higher tensile strength and elastic modulus of aligned nanofibers. The in vitro biocompatibility of aligned and random nanofibrous scaffolds was also assessed by growing mesenchymal stem cells (MSCs), and investigating the proliferation and alkaline phosphatase activity (ALP) on different nanofibrous scaffolds. Our findings showed that the alignment orientation of nanofibers enhanced the osteogenic differentiation of stem cells. The in vitro results showed that the aligned biocomposite nanofibrous scaffolds of PCL/nHA/PVA could be a potential substrate for tissue engineering applications, especially in the field of artificial bone implant.
Wound healing is an important concern these days and there are lots of various medications for it.
One of the most effective methods is Applying Acellular Matrices to the wound bed.
In this presentation, It was tried to explain the application of these products as well as the complications of usage.
I wish it be useful for anyone.
It would be appreciated if you let me know your comments on my presentation.
sincerely,
S. Mohammad Zargar
Contact me: s.mohammad.zargar@gmail.com
This document describes the development of a high content screening assay to evaluate the effects of degradation products from gelatin-based biomaterials on osteoclast differentiation. The assay uses the murine monocytic cell line RAW 264.7, which can differentiate into osteoclasts. Degradation products from six different gelatin-lysine diisocyanate hydrogel compositions were tested in the assay. The degradation products were found to inhibit the formation of multinucleated osteoclasts, suggesting they may support bone regeneration by suppressing bone resorption. The assay provides a way to quantitatively assess biomaterial effects on osteoclast fusion and differentiation in a high throughput manner.
This document reviews the investigation of mechanical properties of chitosan-based films. It discusses how chitosan films are typically produced using either a solvent casting (wet) method or thermo-mechanical (dry) method. The solvent casting method, where a chitosan-acid solution is cast and dried, is most common for producing films for food applications due to advantages like transparency and flexibility. The review summarizes several studies that have investigated the mechanical properties like tensile strength of chitosan films and composites with other materials, finding properties can be improved through the addition of plasticizers or nanomaterials.
This document summarizes new wound management techniques and products. It discusses how selecting appropriate wound care can positively influence repair by understanding repair mechanisms. During the inflammatory phase, debridement and dressings can accelerate healing by enhancing inflammation. Products like Solcoseryl and hydrogels can stimulate early repair but may prolong inflammation later. Sugar, honey, and ketanserin can activate macrophages and improve inflammation. Chronic wounds get "stuck" in inflammation; treatments aim to identify and remove obstacles and create a healing environment. Later, growth factors and dressings that release them can enhance proliferation while limiting scarring.
In Vitro Cell Tests for Functional FoodInstitut Kurz
The relationship between the food we eat and our health is
clear. In the constant search for healthier foods rich in
bioactive compounds that promote health and healthy
ageing, a wide variety of functional foods have appeared on
the market.
To know the real function of these functional foods
in our body, it is necessary to carry out different types of in
vitro cell tests.
Institut Kurz specializes in conducting in vitro cell tests for functional foods.
Contact us for more information:
info@institut-kurz.com
https://www.institut-kurz.com/
Objective: In order to reduce complications accompanied with dental implant restoration, this study strives to prepare a novel sealant and lubricant that can be used in dental implant systems as well as to evaluate its characteristics.
Study Design: Chitosan (CS), β-glycerophosphate pentahydrate (β-GP), and nano silver (nAg) were used to prepare thermosensitive hydrogel. According to the different volume ratios of CS to β-GP, 3 experimental groups were established, namely 16/4, 13/7, and 10/10 groups. Their morphology, composition, and chemical properties were analyzed via SEM, EDS, and FTIR. In addition, the effect of the hydrogel on the stability of dental implant-abutment connection was investigated by removal torque test combined with dynamic cyclic loading experiment. The maximum fracture load was measured under different lubricating conditions by electronic universal testing machine. The cytotoxicity and in vitro antibacterial effect of the hydrogel were examined respectively by CCK-8 test and the spread plate method.
Results: The CS/β-GP/nAg thermosensitive hydro-gel was successfully prepared in this study, which was found to be a porous structure through SEM. The removal torque test and the dynamic cyclic loading experiment showed that the removal torque of the experimental group was greater than that of the control group. Furthermore, the single load-to-fracture test indicated that the 16/4 group had the greatest maximum bearing load. The in vitro cytotoxicity test using rat bone marrow stromal cells (rBMSCs) and human gingival fibroblast cells (hGFCs) showed no cytotoxicity in all 3 groups. The 3 experimental groups had obvious antibacterial effects against E. coli, S. aureus, and P. gingivalis.
Conclusion: A nontoxic antibacterial CS/β-GP/nAg thermosensitive hydrogel for lubricating purpose was successfully fabricated. When the volume ratio of CS to β-GP was 16/4, this thermosensitive hydrogel demonstrated better sealing and lubricating abilities and had a positive influence on the reliability of dental implant-abutment connection.
Keywords: abutment, dental implant, dental implant restoration, dental sealant, lubrication, thermosensitive hydrogel
Aligned and random nanofibrous nanocomposite scaffolds for bone tissue engine...Nanomedicine Journal (NMJ)
Abstract
Aligned and random nanocomposite nanofibrous scaffolds were electrospun from polycaprolactone (PCL), poly (vinyl alcohol) (PVA) and hydroxyapatite nanoparticles (nHA). The morphology and mechanical characteristics of the nanofibers were evaluated using scanning electron microscopy and tensile testing, respectively. Scanning electron microscopy revealed fibers with an average diameter of 123 ± 32 nm and 339 ± 107 nm for aligned and random nanofibers, respectively. The mechanical data indicated the higher tensile strength and elastic modulus of aligned nanofibers. The in vitro biocompatibility of aligned and random nanofibrous scaffolds was also assessed by growing mesenchymal stem cells (MSCs), and investigating the proliferation and alkaline phosphatase activity (ALP) on different nanofibrous scaffolds. Our findings showed that the alignment orientation of nanofibers enhanced the osteogenic differentiation of stem cells. The in vitro results showed that the aligned biocomposite nanofibrous scaffolds of PCL/nHA/PVA could be a potential substrate for tissue engineering applications, especially in the field of artificial bone implant.
Biomaterial is any matter or construct that interacts with biological systems. Biomaterials are often produced in nature or synthesized in the lab using metallic, ceramic, or chemical approaches. They are used for medical applications like implants and prosthetics. Biomaterials must be biocompatible with the human body. The field of biomaterials science incorporates elements of medicine, biology, chemistry, and materials science to develop these materials.
This document discusses applications of composite materials in the biomedical field. It describes how composites consist of two or more materials with distinct properties and are used in biomedical applications like implants, surgical devices, and prosthetics. Specific applications discussed include using carbon fiber composites for orthopedic implants like hip replacements and bone plates, fiber-reinforced dental composites, composite scaffolds for tissue engineering, and composite materials for diagnostic devices and cancer treatment. Important considerations for biomedical composites include biocompatibility, mechanical properties, degradation properties, and aesthetics.
Influence of processing and sterilisation on PCL Polymers, Rubber, Composites...Dr Neill Weir
This document discusses how processing and sterilization can affect the properties of poly-e-caprolactone (PCL), a biodegradable polymer. PCL pellets were either injection molded or extruded and then sterilized using ethylene oxide gas. Testing showed that processing and sterilization did not significantly change the polymer's molecular weight. However, crystallinity was affected. Injection molding increased crystallinity by 5%, and sterilization further increased crystallinity in all materials by approximately 10%. While properties like mechanical strength were not influenced by sterilization, the results suggest that sterilization does cause annealing of PCL and increases its crystallinity.
Synergism effect between inoculum size and aggregate size on flavonoid produc...eSAT Journals
Abstract The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin l-1, flavonoids were not significantly produced in cells or released into the culture medium. Production of flavonoid was correlated with the aggregation size and inoculum density. At aggregate size of 250-500 μm with 0.3 g inoculum density 25 mL-1, it gave the highest luteolin content with 35.45 μg g-1 DW. After investigating the effect of culture conditions, i.e. pH, inoculum density, light irradiation and plant growth regulator, we found that with the optimized condition (i.e. 250-500 μm aggregate size, 0.5 g wet cell 25 mL-1 supplemented with 3 mg L-1 2,4-D and 1 mg L-1 kinetin at pH 5.7 under 16h photoperiod) the luteolin content was 11-fold higher than the cell suspension cultured at an inoculum size of 0.3 g wet cell 25 mL-1 with 250-500 μm aggregate size. Index Terms: Centella asiatica, flavonoid, light irradiation, pH regime, plant growth regulators .
Processing, annealing and sterilisation of poly-l-lactide, Biomaterials, 2003Dr Neill Weir
1) The document examines how processing, annealing, and sterilization affect the properties of poly-l-lactide (PLLA), including its crystallinity, molecular weight, and mechanical properties.
2) It finds that compression molding and extrusion significantly reduced the crystallinity of PLLA pellets, but annealing increased crystallinity. Crystallinity further increased after sterilization with ethylene oxide gas.
3) A slight decrease in molecular weight was observed for extruded PLLA after processing, annealing, and sterilization. Mechanical properties like Young's modulus and tensile strength generally increased with higher crystallinity.
Shao et al-2015-journal_of_biomedical_materials_research_part_b-_applied_biom...Katya Martinez
This document summarizes a study on the fabrication and feasibility of an absorbable diacetyl chitin (DAC) surgical suture for wound healing. DAC was synthesized and characterized. A novel monocomponent multifilament DAC suture was developed and its mechanical properties and degradation behavior were investigated. Functional studies on a linear incisional wound model in rats showed that the DAC suture promoted skin regeneration with faster tissue reconstruction and higher wound breaking strength compared to Vicryl sutures. The results demonstrated the potential of DAC suture for short- or middle-term wound healing applications.
This study investigated the protective effects of Salacia oblanga and quercetin on cyclophosphamide-induced chromosome aberrations in rat bone marrow cells. Rats were treated with Salacia oblanga or quercetin for 15 days, then given cyclophosphamide on days 14 and 15. Cyclophosphamide is known to induce chromosome aberrations and oxidative stress. The study found that quercetin completely prevented cyclophosphamide-induced chromosome aberrations, while Salacia oblanga partially prevented them. Both treatments decreased oxidative stress caused by cyclophosphamide. The results suggest that Salacia oblanga and quercetin can protect against the genotoxic and oxidative effects of cyclophosph
This study optimized gelatin and microbial transglutaminase (mTg) hydrogels for use as cardiac tissue engineering scaffolds. Various concentrations of gelatin and mTg were tested by measuring hydrogel mass changes after incubation. Hydrogels with mTg crosslinker showed less mass change, demonstrating mTg's role in stability. The optimal gelatin and mTg concentrations were found to develop biomimetic hydrogel scaffolds for growing cardiomyocytes.
This document discusses various biomaterial fabrication techniques. It begins with an introduction to biomaterials and their uses. It then describes common materials used as biomaterials and the evolution of biomaterials. Several scaffold fabrication techniques are outlined, including solvent casting, melt molding, gas foaming, freeze drying, and fiber bonding. Limitations of these techniques are noted. Rapid prototyping and nanofabrication techniques that allow for more control and precision are also summarized.
This document summarizes evidence on different types of dialysis membranes. It finds:
1) High-flux membranes are recommended to remove molecules like beta-2 microglobulin and may provide survival benefits for some groups. Synthetic membranes are preferred over cellulosic due to better biocompatibility.
2) The HEMO and MPO studies found mortality benefits for high-flux membranes in some subgroups, like those on dialysis over 3.7 years or with low albumin. However, benefits are unclear overall.
3) High-flux membranes clear beta-2 microglobulin better and may reduce amyloid deposits, though amyloidosis is declining overall. Benefits of biocompatibility are uncertain.
Royal jelly accelerates recovery from oral mucositisBee Healthy Farms
Great news for cancer patients who suffer from oral mucositis. However, individuals undergoing this type of intense therapy also need to consume large quantities of propolis to protect their weakened immune system.
This document describes a method for fabricating porous poly(DL-lactic-co-glycolic acid) (PLGA) microspheres that fuse together at body temperature to form solid porous scaffolds, creating an injectable scaffold system. The microspheres were treated with ethanolic sodium hydroxide to increase surface porosity without disintegrating. When mixed with media and incubated at 37°C, the microspheres fused to form scaffolds with compressive strength of 0.9 MPa, porosity of 81.6%, and pore diameter of 54 μm, supporting NIH-3T3 cell attachment and proliferation in vitro. This study demonstrates a technique for producing an injectable and porous PLGA scaffold that solid
Berberine is an isoquinoline alkaloid found in several plants species. It has a wide range of pharmacological activities including antimicrobial, antidiarrheal, antiprotozoal, hypoglycemic, hypolipidemic, and cardiovascular effects. Specifically, berberine shows efficacy against bacterial diarrhea, intestinal parasites like Giardia lamblia, and ocular trachoma infections. Additionally, berberine has positive effects on lowering blood sugar levels and cholesterol. Due to its broad therapeutic potential and relatively low toxicity, berberine is a promising candidate for further development as an alternative treatment for various infectious and metabolic conditions.
Pluronic F127 gels are proposed as an injectable barrier to prevent epidural scarring after spinal surgery. Rheological tests showed gelation of F127 above 17-18% concentration. Gels containing bovine serum albumin had lower elastic modulus than gels without. Dissolution tests found lower concentration gels dissolved faster when exposed to fluid. A system will be developed to test gel behavior under flow conditions mimicking the spinal region, using a 3D printed spine model and CT scan data. This will help determine if Pluronic gels can effectively serve as a post-surgery scar prevention barrier.
Synthesis and Utility of Starch Based Polymers- A Short Reviewiosrjce
IOSR Journal of Applied Chemistry (IOSR-JAC) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of applied chemistry and its applications. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Chemical Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Dottor Ruszczak collagene per la somministrazione di antibioticiRobertoPalumbo12
In questo studio vengono riassunti alcuni sviluppi e applicazioni del collagene come sistema biologico di somministrazione di antibiotico, in particolare gentamicina.
Le principali applicazioni cliniche includono:
trattamento e profilassi delle infezioni delle ossa e dei tessuti molli, guarigione delle ferite, nonché trattamento oftalmico e parodontale.
Vengono discussi I vantaggi dell'applicazione locale e la logica dell'uso dei sistemi di somministrazione locale del farmaco.
This document summarizes a study investigating the use of graphene (GP), graphene oxide (GO), and Cissus quadrangularis (CQ) callus extract to improve the osteoinductive potential of polycaprolactone (PCL) scaffolds for bone tissue engineering. PCL sheets were coated with combinations of GP, GO, and CQ solutions. The coated scaffolds showed improved roughness, wettability, strength and biocompatibility. Scaffolds containing GO-CQ or GP-CQ promoted osteoblast differentiation of mesenchymal stem cells without osteogenic factors, indicating their potential for bone regeneration. The combination of PCL-GO-CQ performed the best in supporting bone tissue growth.
Mechanical Properties of Tere-Phthalic Unsaturated Polyester Resin Reinforced...IOSR Journals
Abstract: The objective of this work is to investigate the mechanical properties of particulate snail shell
reinforced unsaturated polyester composite. 5wt% ground snail shell of particle size 625microns was
introduced to unsaturated polyester matrix to produce a composite. Other specimens were produced at 10, 15,
20, 25 and 30 weight percentages of the particulate filler in unsaturated polyester matrix. Mechanical tests were
conducted on prepared samples of the composite material. The results showed that the flexural strength of the
composite with 20wt% snail shell particulate reinforcement was greatly enhanced and the impact and hardness
properties were greatly improved at 5wt% filler loading. The composite could be considered for applications in
areas where high impact strength is a requirement such as in shipping containers. The 20wt% snail shell
reinforced unsaturated polyester can be used in place of pure polyester for applications where flexibility is of
utmost importance. Keywords: Snail Shell, Unsaturated Polyester, Composite, Mechanical Properties, filler
Description :
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
Biomaterial is any matter or construct that interacts with biological systems. Biomaterials are often produced in nature or synthesized in the lab using metallic, ceramic, or chemical approaches. They are used for medical applications like implants and prosthetics. Biomaterials must be biocompatible with the human body. The field of biomaterials science incorporates elements of medicine, biology, chemistry, and materials science to develop these materials.
This document discusses applications of composite materials in the biomedical field. It describes how composites consist of two or more materials with distinct properties and are used in biomedical applications like implants, surgical devices, and prosthetics. Specific applications discussed include using carbon fiber composites for orthopedic implants like hip replacements and bone plates, fiber-reinforced dental composites, composite scaffolds for tissue engineering, and composite materials for diagnostic devices and cancer treatment. Important considerations for biomedical composites include biocompatibility, mechanical properties, degradation properties, and aesthetics.
Influence of processing and sterilisation on PCL Polymers, Rubber, Composites...Dr Neill Weir
This document discusses how processing and sterilization can affect the properties of poly-e-caprolactone (PCL), a biodegradable polymer. PCL pellets were either injection molded or extruded and then sterilized using ethylene oxide gas. Testing showed that processing and sterilization did not significantly change the polymer's molecular weight. However, crystallinity was affected. Injection molding increased crystallinity by 5%, and sterilization further increased crystallinity in all materials by approximately 10%. While properties like mechanical strength were not influenced by sterilization, the results suggest that sterilization does cause annealing of PCL and increases its crystallinity.
Synergism effect between inoculum size and aggregate size on flavonoid produc...eSAT Journals
Abstract The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin l-1, flavonoids were not significantly produced in cells or released into the culture medium. Production of flavonoid was correlated with the aggregation size and inoculum density. At aggregate size of 250-500 μm with 0.3 g inoculum density 25 mL-1, it gave the highest luteolin content with 35.45 μg g-1 DW. After investigating the effect of culture conditions, i.e. pH, inoculum density, light irradiation and plant growth regulator, we found that with the optimized condition (i.e. 250-500 μm aggregate size, 0.5 g wet cell 25 mL-1 supplemented with 3 mg L-1 2,4-D and 1 mg L-1 kinetin at pH 5.7 under 16h photoperiod) the luteolin content was 11-fold higher than the cell suspension cultured at an inoculum size of 0.3 g wet cell 25 mL-1 with 250-500 μm aggregate size. Index Terms: Centella asiatica, flavonoid, light irradiation, pH regime, plant growth regulators .
Processing, annealing and sterilisation of poly-l-lactide, Biomaterials, 2003Dr Neill Weir
1) The document examines how processing, annealing, and sterilization affect the properties of poly-l-lactide (PLLA), including its crystallinity, molecular weight, and mechanical properties.
2) It finds that compression molding and extrusion significantly reduced the crystallinity of PLLA pellets, but annealing increased crystallinity. Crystallinity further increased after sterilization with ethylene oxide gas.
3) A slight decrease in molecular weight was observed for extruded PLLA after processing, annealing, and sterilization. Mechanical properties like Young's modulus and tensile strength generally increased with higher crystallinity.
Shao et al-2015-journal_of_biomedical_materials_research_part_b-_applied_biom...Katya Martinez
This document summarizes a study on the fabrication and feasibility of an absorbable diacetyl chitin (DAC) surgical suture for wound healing. DAC was synthesized and characterized. A novel monocomponent multifilament DAC suture was developed and its mechanical properties and degradation behavior were investigated. Functional studies on a linear incisional wound model in rats showed that the DAC suture promoted skin regeneration with faster tissue reconstruction and higher wound breaking strength compared to Vicryl sutures. The results demonstrated the potential of DAC suture for short- or middle-term wound healing applications.
This study investigated the protective effects of Salacia oblanga and quercetin on cyclophosphamide-induced chromosome aberrations in rat bone marrow cells. Rats were treated with Salacia oblanga or quercetin for 15 days, then given cyclophosphamide on days 14 and 15. Cyclophosphamide is known to induce chromosome aberrations and oxidative stress. The study found that quercetin completely prevented cyclophosphamide-induced chromosome aberrations, while Salacia oblanga partially prevented them. Both treatments decreased oxidative stress caused by cyclophosphamide. The results suggest that Salacia oblanga and quercetin can protect against the genotoxic and oxidative effects of cyclophosph
This study optimized gelatin and microbial transglutaminase (mTg) hydrogels for use as cardiac tissue engineering scaffolds. Various concentrations of gelatin and mTg were tested by measuring hydrogel mass changes after incubation. Hydrogels with mTg crosslinker showed less mass change, demonstrating mTg's role in stability. The optimal gelatin and mTg concentrations were found to develop biomimetic hydrogel scaffolds for growing cardiomyocytes.
This document discusses various biomaterial fabrication techniques. It begins with an introduction to biomaterials and their uses. It then describes common materials used as biomaterials and the evolution of biomaterials. Several scaffold fabrication techniques are outlined, including solvent casting, melt molding, gas foaming, freeze drying, and fiber bonding. Limitations of these techniques are noted. Rapid prototyping and nanofabrication techniques that allow for more control and precision are also summarized.
This document summarizes evidence on different types of dialysis membranes. It finds:
1) High-flux membranes are recommended to remove molecules like beta-2 microglobulin and may provide survival benefits for some groups. Synthetic membranes are preferred over cellulosic due to better biocompatibility.
2) The HEMO and MPO studies found mortality benefits for high-flux membranes in some subgroups, like those on dialysis over 3.7 years or with low albumin. However, benefits are unclear overall.
3) High-flux membranes clear beta-2 microglobulin better and may reduce amyloid deposits, though amyloidosis is declining overall. Benefits of biocompatibility are uncertain.
Royal jelly accelerates recovery from oral mucositisBee Healthy Farms
Great news for cancer patients who suffer from oral mucositis. However, individuals undergoing this type of intense therapy also need to consume large quantities of propolis to protect their weakened immune system.
This document describes a method for fabricating porous poly(DL-lactic-co-glycolic acid) (PLGA) microspheres that fuse together at body temperature to form solid porous scaffolds, creating an injectable scaffold system. The microspheres were treated with ethanolic sodium hydroxide to increase surface porosity without disintegrating. When mixed with media and incubated at 37°C, the microspheres fused to form scaffolds with compressive strength of 0.9 MPa, porosity of 81.6%, and pore diameter of 54 μm, supporting NIH-3T3 cell attachment and proliferation in vitro. This study demonstrates a technique for producing an injectable and porous PLGA scaffold that solid
Berberine is an isoquinoline alkaloid found in several plants species. It has a wide range of pharmacological activities including antimicrobial, antidiarrheal, antiprotozoal, hypoglycemic, hypolipidemic, and cardiovascular effects. Specifically, berberine shows efficacy against bacterial diarrhea, intestinal parasites like Giardia lamblia, and ocular trachoma infections. Additionally, berberine has positive effects on lowering blood sugar levels and cholesterol. Due to its broad therapeutic potential and relatively low toxicity, berberine is a promising candidate for further development as an alternative treatment for various infectious and metabolic conditions.
Pluronic F127 gels are proposed as an injectable barrier to prevent epidural scarring after spinal surgery. Rheological tests showed gelation of F127 above 17-18% concentration. Gels containing bovine serum albumin had lower elastic modulus than gels without. Dissolution tests found lower concentration gels dissolved faster when exposed to fluid. A system will be developed to test gel behavior under flow conditions mimicking the spinal region, using a 3D printed spine model and CT scan data. This will help determine if Pluronic gels can effectively serve as a post-surgery scar prevention barrier.
Synthesis and Utility of Starch Based Polymers- A Short Reviewiosrjce
IOSR Journal of Applied Chemistry (IOSR-JAC) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of applied chemistry and its applications. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Chemical Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Dottor Ruszczak collagene per la somministrazione di antibioticiRobertoPalumbo12
In questo studio vengono riassunti alcuni sviluppi e applicazioni del collagene come sistema biologico di somministrazione di antibiotico, in particolare gentamicina.
Le principali applicazioni cliniche includono:
trattamento e profilassi delle infezioni delle ossa e dei tessuti molli, guarigione delle ferite, nonché trattamento oftalmico e parodontale.
Vengono discussi I vantaggi dell'applicazione locale e la logica dell'uso dei sistemi di somministrazione locale del farmaco.
This document summarizes a study investigating the use of graphene (GP), graphene oxide (GO), and Cissus quadrangularis (CQ) callus extract to improve the osteoinductive potential of polycaprolactone (PCL) scaffolds for bone tissue engineering. PCL sheets were coated with combinations of GP, GO, and CQ solutions. The coated scaffolds showed improved roughness, wettability, strength and biocompatibility. Scaffolds containing GO-CQ or GP-CQ promoted osteoblast differentiation of mesenchymal stem cells without osteogenic factors, indicating their potential for bone regeneration. The combination of PCL-GO-CQ performed the best in supporting bone tissue growth.
Mechanical Properties of Tere-Phthalic Unsaturated Polyester Resin Reinforced...IOSR Journals
Abstract: The objective of this work is to investigate the mechanical properties of particulate snail shell
reinforced unsaturated polyester composite. 5wt% ground snail shell of particle size 625microns was
introduced to unsaturated polyester matrix to produce a composite. Other specimens were produced at 10, 15,
20, 25 and 30 weight percentages of the particulate filler in unsaturated polyester matrix. Mechanical tests were
conducted on prepared samples of the composite material. The results showed that the flexural strength of the
composite with 20wt% snail shell particulate reinforcement was greatly enhanced and the impact and hardness
properties were greatly improved at 5wt% filler loading. The composite could be considered for applications in
areas where high impact strength is a requirement such as in shipping containers. The 20wt% snail shell
reinforced unsaturated polyester can be used in place of pure polyester for applications where flexibility is of
utmost importance. Keywords: Snail Shell, Unsaturated Polyester, Composite, Mechanical Properties, filler
Description :
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
- The document is an agenda for a presentation on using WordPress.
- It introduces WordPress standards and templates, and recommends starting with a basic theme to develop WordPress sites.
- The presentation instructs attendees to take one of their web projects, download a basic theme, and insert their project into the theme to get started with WordPress.
NCompass Live - Dec. 23, 2015
http://nlc.nebraska.gov/ncompasslive/
Sally Snyder, Nebraska Library Commission's Coordinator of Children and Young Adult Library Service, and Jill Annis, Librarian at Elkhorn (NE) Grandview Middle School, will give brief book talks on new titles that could be good additions to your library's collection. Titles for middle and high school ages will be included.
WordCamp Bilbao - De HTML a WordPress - @DarioBFDarío BF
Aprende cómo pasar tu maqueta HTML y CSS a tema de WordPress.
Un amplio repaso a la estructura de un theme de WordPress y las buenas prácticas a la hora de desarrollar nuestro propio theme.
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Biomaterials were defined as “any substance, other than a drug, or a combination of substances, synthetic or natural in origin, which can be used for any period of time, as a whole or as a part of a system, which treats, augments or replaces any tissue, organ or function of the body”
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engineering applications including skin, cartilage, bone, liver,
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is considered one of the highest potential biomaterials for
impending trends in which the gelatin-based scaffolds are used
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Indian Dental Academy: will be one of the most relevant and exciting training center with best faculty and flexible training programs for dental professionals who wish to advance in their dental practice,Offers certified courses in Dental implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic Dentistry, Periodontics and General Dentistry.
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(b)
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1. Dense chitosan surgical membranes produced by a coincident
compression-dehydration process
Thomas P. Dooley*, April L. Ellis, Maria Belousova, Don Petersen and Arthur A. DeCarlo
Agenta Biotechnologies Inc, 1500 1st Avenue North, Unit 31, Birmingham, AL 35203, USA
(Received 16 April 2012; accepted 7 June 2012)
High density chitosan membranes were produced via a novel manufacturing process and
used as implantable resorbable surgical membranes. The innovative method utilizes the fol-
lowing three sequential steps: (1) casting an acidic chitosan solution within a silicon mold,
followed by freezing; (2) neutralizing the frozen acidic chitosan solution in alkaline solu-
tion to facilitate polymerization; and (3) applying coincident compression-dehydration
under a vacuum. Resulting membranes of 0.2–0.5 mm thickness have densities as high as
1.6 g/cm3
. Inclusion of glycerol prior to the compression-dehydration step provides addi-
tional physical and clinical handling benefits. The biomaterials exhibit tensile strength with
a maximum load as high as 10.9 N at $2.5 mm width and clinically relevant resistance to
suture pull-out with a maximum load as high as 2.2 N. These physical properties were
superior to those of a commercial reconstituted collagen membrane. The dense chitosan
membranes have excellent clinical handling characteristics, such as pliability and ‘memory’
when wet. They are semipermeable to small molecules, biodegradable in vitro in lysozyme
solution, and the rates of degradation are inversely correlated to the degree of deacetyla-
tion. Furthermore, the dense chitosan membranes are biocompatible and resorbable in vivo
as demonstrated in a rat oral wound healing model. The unique combination of physical,
in vitro, in vivo, and clinical handling properties demonstrate the high utility of dense
chitosan membranes produced by this new method. The materials may be useful as surgical
barrier membranes, scaffolds for tissue engineering, wound dressings, and as delivery
devices for active ingredients.
Keywords: chitosan; membrane; wound healing; biodegradation; resorbable; mechanical
properties; suture
1. Introduction
The goal of this work was to develop a biocompatible, suture-able, and resorbable membrane
material that exhibited excellent physical, in vitro, and in vivo properties indicative of high
clinical utility as a barrier membrane. In order to develop a new implantable surgical mem-
brane, one should first be aware of the routine indications of use and features of commercial
materials used for these indications. Implantable surgical membranes typically have the fol-
lowing uses and properties: (a) to separate tissue layers; (b) to augment tissue defects or defi-
ciencies; (c) to complement bone graft substitutes; (d) to prevent adhesions; (e) to provide
strength; (f) to repair or prevent hernias; and (g) to promote blood clotting. Therefore, an
*Corresponding author. Email: dooley@agentabio.com
Journal of Biomaterials Science, Polymer Edition
2012, 1–23, iFirst Article
ISSN 0920-5063 print/ISSN 1568-5624 online
Ó 2012 Taylor & Francis
http://dx.doi.org/10.1080/09205063.2012.701549
http://www.tandfonline.com
2. ideal new biomaterial intended for use as a suture-able surgical membrane should proficiently
address some or all of these in vivo uses and properties.
Several biocompatible resorbable source materials have been employed in US
FDA-cleared medical devices as commercial surgical membranes, most notably reconstituted
mammalian collagen. Other biomaterials include polymers or co-polymers of lactic acid and
glycolic acid (e.g. PLGA), and processed xenograft skin or gastrointestinal tissue. Of these,
the most common implantable surgical barrier membranes in use today by physicians and
dentists are reconstituted bovine or porcine collagens. However, the physical and clinical han-
dling characteristics of commercial collagen membranes at times display ‘weaknesses’ (literal
and figurative) and can fail to meet the needs of clinicians. For instance, once wetted collagen
membranes tend to lose sufficient strength and contour ‘memory.’ In some cases, the materi-
als are not sufficiently strong enough to support or maintain sutures or tacks to anchor the
membrane into the tissues. Furthermore, the collagen material often absorbs blood rapidly
during placement making it difficult to distinguish between the implant material and the surgi-
cal site.
Note that there are other types of resorbable biomedical membranes in the scientific litera-
ture, for instance electrospun/nanofibrous, laminated, wound dressing, and/or drug-eluting
membranes. However, unlike those materials, our primary focus is upon surgical barrier mem-
branes intentionally designed with clinical strength and suture-ability in mind. Thus, our
objective was to develop a biocompatible, suture-able, and resorbable material for use in med-
ical devices that overcomes the inadequacies or deficiencies of the clinical standard-of-care
collagen and other commercial resorbable barrier membranes.
In order to overcome some of the ‘weaknesses’ in commercially available surgical mem-
branes, we have turned our attention to another biocompatible nontoxic material, purified
chitosan. This material has well-established physical characteristics [1,2] and a history of
safety and efficacy in human subjects [3]. Chitosan is a linear polysaccharide consisting of
beta(1,4) linked N-acetylglucosamine and glucosamine (GlcN) monosaccharides [1]. Chitin,
from which chitosan is derived, is nature’s second most abundant biopolymer. Chitosan is
routinely derived from the exoskeleton of marine crustaceans by a multistep process of depo-
lymerization/decalcification in acid, deproteinization, and N-deacetylation in alkali.
Chitosan degrades in vivo by enzymatic processes into harmless GlcN and related oligo-
saccharide units. Chitosan is subject to enzymatic degradation within mammals by lysozyme,
NAGases, chitinases [4–7], and possibly alpha-amylase [8]. Some of these enzymes are impli-
cated in antibacterial innate immunity, in which the enzymes (for instance produced by mac-
rophages) attack the polysaccharides of bacterial cell walls. Chitosan is coincidentally subject
to the same degradative processes. In addition, in vivo resorption might also include the pro-
cess of phagocytosis/endocytosis of chitosan nano-particulates by host cells, such as macro-
phages. The rates of enzymatic biodegradation in vitro and resorption in vivo vary based
upon the percentage of deacetylation (% DD). Fully deacetylated chitosan does not serve as a
substrate for enzymatic degradation by lysozyme. Thus, the rate of degradation is inversely
proportional to the % DD. This feature can be manipulated to control the rates of biodegrada-
tion in vivo, and without the requirement for a cross-linking agent.
With regard to biocompatibility per se, purified chitosan has been demonstrated to be safe
(and effective) in humans and other mammals [3,9], as exemplified by seven broad contexts:
(1) US FDA-cleared medical devices for humans: Purified chitosan is a component in
multiple US FDA-cleared Class I medical devices. In most cases, chitosan is the prin-
cipal component in the hemostatic wound dressings [10], and in some forms also
serves as a physical barrier [11].
2 T.P. Dooley et al.
3. (2) GRAS food additive for humans: Chitosan is considered as generally recognized as
safe (GRAS) as a food additive at the level of ‘self-affirmed’ by various manufacturers
(e.g. Primex). To the best of our knowledge, a GRAS designation at the higher level
of ‘no comment’ following a full US FDA review has not yet occurred.
(3) Cosmetic & consumer skincare products for humans: Chitosan is listed among the
International Nomenclature of Cosmetic Ingredients. Chitosan and its various salt
forms (e.g. lactate, glycolate, ascorbate, formate, and salicylate) and other organic
derivatives are listed as ingredients for use in cosmetics and consumer skincare prod-
ucts. To the best of our knowledge, chitosan has not warranted consideration by the
Cosmetics Ingredients Review expert safety panel.
(4) Purified chitosan is nonallergenic in humans: A very small portion of the human
population exhibits allergies to marine crustaceans, with estimates at approximately
0.2–0.3% [12,13]. This has led to the question of whether individuals with preexisting
allergies to marine crustaceans should avoid using medical devices composed of crus-
tacean-derived purified chitosan. A respected authority on chitosan, Dr. Riccardo Muz-
zarelli reported, “The major shrimp allergen has been identified as the muscle protein
tropomyosin … Shrimp-derived glucosamine is safe even for individuals hypersensitive
to tropomyosin … Gray et al. clearly state that ‘shellfish allergy is caused by IgE anti-
bodies to antigens in the flesh of the shellfish and not the shell; therefore it should be
safe for patients with shellfish allergy to take glucosamine supplement’” ([14], p. 300).
Furthermore, “In experimental and pre-clinical surgical trials¸ the use of chitin/chito-
san and their derivatives has never led to allergies or other diseases” ([14], p. 304).
In addition, according to a clinical study summary from the University of Kuopio
(provided to us by Primex ehf), humans were subjected to cutaneous allergy prick tests
using purified chitosan. None of the subjects yielded a positive response to chitosan,
even among the subset of individuals who tested positive for shrimp allergic reactions
and/or shrimp-specific IgE. Thus, purified chitosan is nonallergenic.
(5) Wound healing in humans: Beyond US FDA Class I wound dressings, chitosan has
also been used as a biocompatible material with success in vivo in a variety of human
wound-healing investigations. As examples, in a periodontal study a chitosan gel pro-
duced a radiographic benefit to bone relative to the control treatment, thus demonstrat-
ing a guided bone regeneration benefit [15]. In another periodontal study, chitosan
ascorbate gel provided an efficacious result for periodontitis [16]. Furthermore, chito-
san membranes were used with benefit to promote the healing of cutaneous wounds
[17] and nasal sinuses [18].
(6) Wound healing in mammals: It has been well documented that chitosan-containing
devices have been used with success in vivo in veterinary care, in wound healing, and
in tissue engineering studies in a vast array of nonhuman mammalian species and
organ sites [19,20].
(7) Delivery of active ingredients: Chitosan has been used successfully in vivo as con-
trolled release matrices for the delivery of drugs, biologics, peptides, nucleic acids,
and vaccines [3,21–44].
The historic challenge before us was to develop a process whereby purified chitosan could
be formed into high density membranes that coincidentally retained appropriate physical and
clinical handling properties suitable for use as a surgical membrane. None of the chitosan
materials to date in the prior art had this unique desirable blend of attributes. Other chitosan
biomaterials have been developed and, in some cases, commercialized. However, none of
these methods of manufacture delivered appropriate properties for use as surgical membranes,
Journal of Biomaterials Science, Polymer Edition 3
4. most notably: (a) high density; (b) pliability without fragility; (c) tensile strength; and (d)
resistance to suture pull-out. For instance, a common commercial material is the low density
chitosan ‘sponge’ that is routinely used as a wound dressing for hemostasis. This product
lacks sufficient density and strength to serve as a barrier membrane. In addition, it is too thick
(c. 4.5 + mm) and lacks adequate suture-ability for use as an implantable barrier membrane,
which should be preferably about 0.2–0.5 mm in thickness, based upon commercially avail-
able resorbable collagen membranes. Therefore, herein we describe an innovative method for
producing high density chitosan membranes by a coincident compression-dehydration process,
and demonstrate for the first time a resulting suture-able chitosan-based barrier membrane
with multiple beneficial properties of direct relevance to clinical utility.
2. Methods
2.1. Materials
Purified chitosan materials were obtained commercially from Heppe Medical Chitosan GmbH
(Germany) and Primex ehf (Iceland). The batches with degrees of deacetylation from 70 to
95% and viscosities ranging from 416 to 621 mPas (both properties were determined by the
vendors) are listed in Table 1. For comparison to a commercially available surgical barrier
membrane, a common reconstituted bovine Type I collagen material was obtained, the Bio-
Mend Absorbable Collagen Membrane® (Zimmer). For comparison to a nonmembrane mate-
rial composed of low density chitosan, the HemCon Dental Dressing® (HemCon Medical
Technologies) was obtained.
2.2. ‘Standard Method’ of manufacturing dense chitosan membranes
Although various permutations were used during the development of the method, the standard
method of manufacture of dense chitosan membranes involved the following steps:
(1) Four percent of w/v purified chitosan base was prepared at room temperature in 2%
v/v acetic acid for the Heppe chitosan solution (or 1% acetic acid v/v for the Primex
chitosan solution) and allowed to stand for a period of $7 days. For moderate thick-
ness membranes, 0.5 g (wet weight) of acidic chitosan solution was cast per cm2
of sil-
icon mold surface area. To do so, 116 g (wet weight) of acidic chitosan solution was
poured into a 6″  6″ (15.2 cm  15.2 cm = 231.04 cm2
) silicone mold, yielding 0.502 g
(wet weight)/cm2
. This material’s surface area will shrink in subsequent steps to
Table 1. Chitosan source materials.
Vendor – brand Label % DD Viscosity (mPa s)
Heppe – Chitoceuticals 70/500 72.1 416
Heppe – Chitoceuticals 75/500 73.8 458
Heppe – Chitoceuticals 80/500 80.1 621
Heppe – Chitoceuticals 85/500 86.5 473
Heppe – Chitoceuticals 90/500 90.8 418
Heppe – Chitoceuticals 95/500 95.2 449
Primex – ChitoClear cg400 75 453
Primex – ChitoClear cg400 79–95⁄
463
⁄
Viscosities determined by alternative methods
4 T.P. Dooley et al.
5. $5.5″  5.5″ ($14 cm  14 cm = 196 cm2
) yielding the equivalent of $0.592 g
(wet weight)/cm2
, which at 4% chitosan in the acidic solution is equivalent to
$23.67 mg (dry weight of chitosan)/cm2
. For a thinner material, casting 0.3 g (wet
weight of 4% chitosan solution) per cm2
of silicon mold surface area would yield
$14.2 mg (dry weight of chitosan)/cm2
. As desired, one may vary the amount of cast
material and/or the percentage of acidic chitosan solution.
(2) The viscous acidic chitosan solution within the mold was subjected to mechanical
vibration on a horizontal platform shaker for 20 min in order to remove air bubbles
and to settle the gel into a uniform thickness.
(3) The mold was subjected to ultra-freezing at À80 °C for 1 h.
(4) The frozen acidic chitosan solution was removed from the silicon mold and immersed
in 2 M NaOH solution at RT for 24 h with gentle agitation, in order to displace the
acid within the composition and to promote polymerization.
(5) The alkali-treated polymerized material was rinsed in slow-flowing deionized water for
24 h to remove all of the alkali. This results in a material with a neutral pH. Then, the
rinsed chitosan polymer was immersed in 10% glycerol in water (v/v) for 15 min. The
inclusion of glycerol prior to coincident compression-dehydration (Step 6) has benefit
to the resulting material, such as flexibility and ease of cutting to size while dry.
(6) The polymerized chitosan material was heat-sealed on all four sides between a pair of
Cellophane® semipermeable membranes (Innovia Films and NE coated). The seals
should be in close proximity to the edges of the chitosan polymer, in order to mini-
mize the void that could permit lateral expansion upon compression. The polymerized
chitosan within the sealed cellophane layers was soaked in deionized water for 15 min
to wet the cellophane, and then placed on a coincident compression-dehydration gel
dryer (Labconco). Within this device a ‘sandwich’ was prepared consisting of an
uppermost flexible rubber seal (to maintain a vacuum), a solid smooth planar material
(e.g. a metal plate), a filter paper (Whatman), the sealed and wetted cellophane ‘enve-
lope’ containing the polymerized chitosan composition, another Whatman filter paper,
and the gel dryer’s mesh surface underneath from which the vacuum is applied. The
vacuum pressure (preferably with a minimal pressure of 25 inches of Hg) was applied
for 4 h in conjunction with coincident heating of the gel dryer at 80 °C.
(7) The vacuum-dried dense chitosan membrane material was separated from the cello-
phane films. The dehydrated chitosan membranes made according to these parameters
are typically 0.2–0.5 mm in thickness and have densities > 0.8 g/cm3
and can reach as
high as 1.6 g/cm3
. The membranes may be stored at room temperature.
2.3. ‘Alternative Method’ of manufacturing dense chitosan membranes
The alternative method lacks glycerol after rinsing (Step 5), and this aspect is referred to as the
alternative “Step 5b”. Whenever Step 5b was performed the subsequent process of soaking in
deionized water to wet the cellophane (Step 6) was increased from 15 min to 1 h duration. How-
ever, it is uncertain whether the increase in time is necessary or beneficial. Membranes produced
without glycerol (i.e. Step 5b) have the tendency to be brittle (when dry) and can inadvertently
crack while cutting to the desired size. In view of this observation, the resulting dry membranes
lacking glycerol were hydrated in deionized water followed by lyophilization (freezing followed
by vacuum without compression), prior to cutting to size. This hydration/lyophilization modifi-
cation is referred to as the additional ‘Step 8.’ In summary, the alternative method of manufac-
turing includes the hydration/lyophilization steps in lieu of glycerol.
Journal of Biomaterials Science, Polymer Edition 5
6. 2.4. Scanning Electron Microscopy
The surfaces of the dense chitosan membranes were sputter coated with gold-palladium and
then examined using an FEI Quanta FEG 650 model scanning electron microscope with sec-
ondary electron imaging and 10 kV accelerating voltage at magnifications of 100Â, 250Â,
and in some instances higher magnifications. An internal reference size bar of 500 um
(0.5 mm) was included in the photographs. Dense chitosan membranes were manufactured by
the standard method (with 10% glycerol) using 75 and 80% DD (Heppe) chitosan at 4% and
0.3 g/cm2
(amount cast). The low density commercial acidic chitosan ‘sponge’ material, Hem-
Con Dental Dressing®, was also examined for comparison. The opposite planar surfaces of
the membranes were annotated according to differences during the manufacturing: (a) frozen
up, dehydrated up and (b) frozen down, dehydrated down. In other words, ‘frozen down’
refers to the orientation of the acidic chitosan solution in contact with the latex mold surface
during casting, and ‘dried down’ refers to the orientation of the neutralized/polymerized chito-
san gel on the side from which the vacuum was drawn.
2.5. Tensile strength
The membrane materials made with Heppe chitosan were tested using an Instron 5565
machine with ASTM standard methodologies and minor modifications (e.g. semi-circular
rather than semi-oval template cut-outs for tensile testing according to ASTM D 1708-06a)
[45,46]. The barbell-shaped dense chitosan membrane strips with $2.5 mm minimum width
were hydrated in phosphate buffered saline (PBS) for 5 min prior to testing on the Instron
machine. The test strips were anchored by pneumatic clamps and fine wet or dry sandpaper.
Replicates (two or three samples per experiment) were analyzed at a crosshead speed of
1 mm/min. The following parameters were determined: thickness while dry (by digital caliper
in mm), minimum width while dry (by digital caliper in mm), maximum load (kgf and N),
tensile extension at maximum load (mm), and maximum tensile stress (kgf/cm2
and MPa).
The conversion factor for kgf to N (Newtons) is 1 kgf = 9.80665 N. Averages of the replicates
within each experiment were calculated for each parameter. A commercial reconstituted colla-
gen membrane control, the BioMend Absorbable Collagen Membrane®, was included for
comparison.
2.6. Resistance to suture pull-out
The membrane materials made with Heppe chitosan were tested using an Instron 5565
machine to determine the resistance to suture pull-out. Strips of $5.0 mm in width were
hydrated in PBS for 5 min prior to testing on the Instron machine. The strips were subjected
to suture pull-out tests, with a single suture loop created using 4.0 silk suture and FS-2 nee-
dle. The suture needle was inserted at the midline of the strip and several millimeters from
the terminus. One end of the test strip was anchored by a pneumatic clamp and fine wet or
dry sandpaper, and the suture loop was attached to a mechanical hook. Replicates (two or
three samples per experiment; with an exception in experiment # 24 consisting of only one
sample) were analyzed at a crosshead speed of 1 mm/min. The following parameters were
determined: thickness (by digital caliper in mm), minimum width (by digital caliper in mm),
maximum load (kgf and N), tensile extension at maximum load (mm), and maximum tensile
stress (kgf/cm2
and MPa). Averages of the replicates within each experiment were calculated
for each parameter. As a nonchitosan control the BioMend Absorbable Collagen Membrane®
was included.
6 T.P. Dooley et al.
7. 2.7. In vitro permeability
Dense chitosan membranes were subjected to Franz cell permeability testing with low molecular
weight dye compounds, Methylene Blue (Mw 285; Fisher Scientific) and Crystal Violet (Mw
373; Sigma) [21]. Membrane discs of $2 cm were hydrated overnight in 0.5Â PBS + sodium
azide, then mounted between the upper and lower chambers of 9 mm (inside diameter) Franz
cells (PermeGear). The stock solution consisted of 0.1% (w/v) dye in 0.5Â PBS + sodium azide
followed by 0.45 micron filtration. 1.0 ml of the filtered dye concentrates (equal to or less than
0.1%) were placed on the top chamber and 5.0 ml of 0.5Â PBS + sodium azide on the lower
receiver chamber. The water-jacketed chambers were maintained at 30 °C using a recirculating
water bath. Passive diffusion across the membranes was monitored by serial sampling of 100 ul
aliquots from the lower 5 ml chamber at 1, 2, and 4 h, and overnight (e.g. 24 h). The samples
taken from the lower chamber and the controls (buffer alone and a dilution series of the starting
dye stocks) were loaded into 96-well plates and subjected to spectrophotometry at 668 and
590 nm for Methylene Blue and Crystal Violet, respectively. The pairs of samples at each time
point were averaged and then subjected to scatter plot regression analyses to determine the initial
linear rates of diffusion during the time interval of 1–4 h.
2.8. In vitro degradation
In order to assess the biodegradability of the dense chitosan materials, 12 mm discs of mem-
branes (Heppe chitosans at various % DD) were placed in multi-well plates and immersed in
15 mg/ml lysozyme solution in PBS + sodium azide for up to 3 weeks at 37 °C (with daily
exchange of the solution). Photographs of disc translucency were obtained at various time
points and were analyzed by Image J imaging software as a measure of loss of chitosan.
2.9. In vitro cell culture
Cellular adhesion and proliferation were investigated on the surfaces of the dense chitosan
membranes (Heppe 80% DD, 0.3 g/cm2
cast amount). Membranes were precisely cut with
8 mm biopsy punches. Membranes were wetted for 10 min with a low volume of appropriate
medium for each cell line. Membranes were then temporarily ‘glued’ to the bottom of a ster-
ile culture plate well with 10 μl of 10% gelatin, warmed to 42 °C. After the gelatin hardened
to adhere the membrane discs to the plastic, 1 Â105
cells were applied per 8 mm disc. Cells
were triturated (monodispersed) three times, allowed to settle for 20 min, and then incubated
at 37 °C with 5% CO2. Three human cell types were used: human primary keratinocytes
(Lifeline Cell Technologies) cultured in DermaLife (Lifeline Cell Technologies); human pri-
mary dermal foreskin fibroblasts (ATCC) cultured in DMEM-high glucose, 5% Fetal Calf
Serum, 1% penicillin-streptomycin; and human MC3T3 preosteoblasts subclone E4 (ATCC)
cultured in Alpha MEM without aspartic acid (Invitrogen). Cell attachment and growth was
analyzed during the next seven days using Cell Tracker Red live cell fluorescent stain (Invit-
rogen) and fluorescence microscopy.
2.10. In vivo biocompatibility & resorption
A rat oral palate surgical model was used to assess in vivo biocompatibility, wound healing,
and resorption. All animal studies were performed with IACUC approval at the University of
Alabama at Birmingham. NIH guidelines for the care and use of laboratory animals (NIH Pub-
lication #85-23 Rev. 1985) have been observed. Bilateral full-thickness 1.5 mm punch wounds
Journal of Biomaterials Science, Polymer Edition 7
8. were made on the palate of male Lewis rats and dense chitosan membranes, control mem-
branes, or no membranes were placed at the base of the wounds. To create the wounds and
insert the membranes, a 4–5 mm linear palatal incision perpendicular to the ridge was made
7 mm mesial to the first molar across the maxillary arch from which a full thickness tunnel flap
was raised along the ridge distally to the first molar. A precise 1.5 mm diameter circular biopsy
punch (Robbins Instruments, Chatham, NJ, USA) was used to create a full-thickness window
in the flap. A 4 mm diameter membrane was slid under the flap so the membrane was exposed
at the punch site toward the oral cavity. The membranes were manufactured by the alternative
method (Primex 79–95% DD, 0.5 g/cm2
cast amount). The flap was closed along the ridge at
the mesial incision with surgical cyanoacrylate and photographed. The animals were main-
tained on a soft diet with acetaminophen analgesic in the drinking water for one week after
surgery. Membranes were placed bilaterally with this procedure, each side requiring approxi-
mately 5–10 min operating time. Photographs were taken of the initial exposed membranes on
the maxilla, then again at days 1, 2, and 3 in all animals, then at 1, 3, 6, and 12 weeks at the
time of euthanasia of groups of animals and assessed for wound closure. Histologic assess-
ments included re-epithelialization, membrane biodegradation, and collagen synthesis at the
site of membrane resorption using Masson’s trichrome stain.
3. Results
Dense chitosan membranes were produced using an innovative manufacturing process, and
two variants of the general method were used to produce a dry and pliable dense material.
The ‘standard method’ included glycerol prior to coincident compression-dehydration. The
‘alternative method’ lacked glycerol, but included an additional final step of hydration-lyophi-
lization. The resulting physical, in vitro, and in vivo properties of the new materials are
described below.
3.1. Physical properties
3.1.1. Density
Membrane densities were calculated based upon the weights of 7 mm punched discs and digi-
tal micrometer measurements of thickness. Depending on the manufacturing variables (e.g.
standard method v. alternative method, cast amount of acidic chitosan solution, and percent-
age of DD), the membranes had dry densities of $1.0–1.6 g/cm3
. The results of representative
experiments (18 experimental batches of chitosan membranes) are summarized in Fig. 1(a).
The dry membranes that were characterized for this study were typically 0.2–0.5 mm in thick-
ness (with one exception out of 18 batches). The standard method (with glycerol) produces a
slightly thinner and denser material than the alternative method. During hydration the dense
chitosan membranes of both methods expanded, more than doubling in thickness within 1 h
(Fig. 1(b)) with no significant increase in thickness beyond 1 h (linear regression, R2
= 0.98).
This is comparable to the degree of hydration expansion of BioMend Absorbable Collagen
Membrane®, and especially so for the standard method (Fig. 1(b)).
The high density chitosan membranes displayed various properties unlike any chitosan
materials disclosed in the prior art or commercially available. For example, the density of
lyophilized acidic chitosan ‘sponge,’ HemCon Dental Dressing® was $0.08 g/cm3
(Fig. 1
(a)). Once wet, the HemCon material produced a gelatinous amorphous material, so it was
not possible to determine the degree of hydration expansion, if any. In view of its low density
and behavior when wet, this material is not suitable or intended for use as an implantable
8 T.P. Dooley et al.
9. suture-able barrier membrane. Rather, it is a wound dressing intended primarily to control
hemorrhage.
3.1.2. Scanning Electron Microscopy
Both surfaces of the dense chitosan membranes have been examined by scanning electron
microscopy (SEM). Photographs of a dense chitosan membrane manufactured by the standard
Figure 1. Densities and increases in membrane thicknesses upon hydration: (a) The dry (prehydration)
densities of four materials (i.e. HemCon Dental Dressing®, BioMend Absorbable Collagen
Membrane®, dense chitosan membranes produced by the alternative and standard methods) measured
directly as a function of mass and volume. (b) The increases in material thickness after hydrating the
membranes for 1 h. Note that it is not possible to measure the re-hydrated HemCon Dental Dressing®,
as it is gelatinous and amorphous lacking sufficient strength. The error bars represent the Standard Error
of the Mean.
Journal of Biomaterials Science, Polymer Edition 9
10. method (Heppe 80% DD, 0.3 g/cm2
cast amount) are shown in Fig. 2(a) and (b). The images
of both planar surfaces provided evidence of smooth surfaces at lower magnifications and
some fine detail porosity at higher magnification. A minor asymmetry was noted between the
two surfaces with regard to porosity.
By contrast the low density commercial chitosan ‘sponge,’ HemCon Dental Dressing®,
displayed higher porosity, with very large pores on one surface (Fig. 2(c)), some of which are
nearly 0.5 mm in breadth. The opposite surface resembled a sponge with considerably smaller
pores (Fig. 2(d)). This material, produced by lyophilization of an acidic chitosan solution, dis-
played profound asymmetry and it is likely that the pores or cavities represent the areas for-
merly occupied by ice crystals. Note that both surfaces of the HemCon material are
remarkably less dense than our chitosan membranes. Our chitosan membranes were almost
entirely solid polymeric material with minimal (and small) pores, whereas the HemCon
‘sponge’ material was the opposite with the majority of the space consisting of air.
Figure 2. SEM at 250Â magnification: (a) A planar surface of a dense chitosan membrane (Heppe
80% DD, 0.3 g/cm2
cast amount, standard method); (b) Same material as (a), but the opposite planar
surface; (c) HemCon Dental Dressing®, surface with very large pores; and (d) Same material as (c), but
the opposite surface with smaller pores. Scale bars = 500 microns.
10 T.P. Dooley et al.
11. 3.1.3. Tensile strength
The tensile strength results are shown in Table 2. An Instron 5565 machine was used to char-
acterize the dense chitosan membranes as barbell-shaped tensile testing strips with a minimum
width of $2.5 mm. The standard method of manufacturing (i.e. eight experimental batches,
with glycerol) was compared to the alternative method (10 experimental batches, with hydra-
tion/lyophilization and lacking glycerol). Other variables included % DD ranging from 70 to
95%, the amount of acidic chitosan solution cast into the mold ranging from 0.25 to 0.5 g/cm2
,
and the dry membrane thicknesses typically ranging from $ 0.2 to 0.5 mm. Note that 0.3–
0.35 g/cm2
of acidic chitosan solution at casting produced ‘thin’ membranes with an average
membrane thickness of 0.23 mm for the standard method (with 10% glycerol). As expected,
the thicknesses of the various membrane batches positively correlated with the amount of
acidic chitosan solution cast into the silicon molds (i.e. R2
values of 0.68 for the standard
method and 0.70 for the alternative method of manufacturing).
The barbell-shaped samples with $2.5 mm in minimum width were hydrated for 5 min
immediately prior to testing on the Instron machine. This step was performed to mirror
how the barrier membranes are intended to be used in a clinical setting by a physician or
dentist. An array of experimental variables (in Table 2) yielded the following ranges of
physical properties: (a) maximum loads of 3.2–10.9 N for the standard method and 2.4–
5.4 N for the alternative method of manufacturing and (b) maximum stresses of 61.7–
118.3 mPa for the standard method and 33.1–68.1 mPa for the alternative method of manu-
facturing. The maximum stress (accounting for cross-sectional area at the narrowest point)
yielded a group average of 85.8 mPa for the eight experimental batches of the standard
method of manufacturing, whereas the alternative method (including postmanufacturing
steps of hydration/lyophilization and lacking glycerol) yielded a group average of 45.2 mPa
for the 10 experimental batches.
Correlation analyses were conducted probing the variables of input composition and
empirically derived results, and analyzed separately for each of the two methods of manufac-
turing, i.e. the standard method and the alternative method. In other words, the standard
method materials were compared among themselves (eight experiments) as a group, and the
alternative method materials were compared among themselves (10 experiments) as another
group. As expected, the dense chitosan membranes of the standard method of manufacturing
(with glycerol) displayed a strong positive correlation (R2
= 0.74) for thickness v. tensile
strength expressed as maximum load. The alternative method of manufacturing (hydration/
lyophilization) also yielded a positive, albeit moderate, correlation (R2
= 0.43) for thickness v.
tensile strength expressed as maximum load.
Note that an additional variable (other than thickness or the amount cast) was the % DD,
and it might account for reducing the anticipated R2
values. In fact, this was demonstrated in
part using six samples produced with 0.25 g/cm2
cast amount (and the alternative method)
that enabled us to assess the effect of the DD (ranging from 70 to 95%) on the strength. The
maximum loads for this set of six matched samples were negatively correlated with increasing
% DD (R2
= 0.65). In other words, higher deacetylation makes the polymeric material weaker.
The new chitosan materials were compared to a commercial surgical barrier membrane,
the reconstituted bovine Type I collagen membrane, BioMend Absorbable Collagen Mem-
brane® (Table 2). The ‘thin’ dense chitosan membranes of the standard method cast using
0.3–0.35 g/cm2
of 4% acidic chitosan solution exhibit more than double the maximum load
compared to this reference reconstituted collagen membrane (i.e. average of 4.74 N v.
2.24 N). In this comparison, the thicknesses of the two types of materials were similar (i.e.
average of 0.23 mm for chitosan v. 0.17 mm for the collagen).
Journal of Biomaterials Science, Polymer Edition 11
13. 3.1.4. Resistance to suture pull-out
The resistance to suture pull-out results are shown in Table 2. Using an Instron 5565
machine, $5.0 mm strips of the membrane were subjected to suture pull-out tests. In an array
of experimental variables, the membranes exhibited resistance to suture pull-out with maxi-
mum loads as high as 2.2 N. Note that all samples were hydrated for 5 min immediately prior
to testing on the Instron machine. This step was performed to mirror how the materials are
intended to be used in a clinical setting by a physician or dentist.
Using either our standard method or the alternative method with varying cast amounts
(0.25–0.5 g/cm2
) the maximum load for resistance to suture pull-out exhibited similar ranges
(both group averages were 1.4 N). In aggregate the two groups ranged from 0.92 to 2.2 N,
and demonstrated by linear regression analysis a positive, albeit moderate, correlation between
thickness and max load (R2
= 0.396).
Suture pull-out test results show the dense chitosan membranes exhibit nearly double the
suture pull-out strength of a commercial reconstituted collagen membrane, BioMend Absorb-
able Collagen Membrane® (0.75 N).
3.2. In vitro properties
3.2.1. Permeability
The chitosan membranes were subjected to Franz cell permeability testing with small molecule
dye compounds [21]. The dense chitosan membranes permitted the passive diffusion of small
molecules, such as methylene blue and crystal violet dyes, with molecular weights of 285 and
373, respectively. Duplicate membrane samples were tested at 30 °C, and the pairs of data points
were averaged. Two separate experiments are demonstrated in Table 3.
The initial linear rates of diffusion across the membranes (at 1–4 h) were calculated as a
function of the amount of starting dye material v. time in hours. The linear regression func-
tions were robust in all cases with R2
values ranging from 0.93 to 1.0. As expected, in the
first experiment using 80% DD chitosan (Heppe) the initial rate of flux of Crystal Violet was
higher for the 0.3 g/cm2
amount cast than for the 0.5 g/cm2
amount cast. Similarly, the rate of
flux for methylene blue was higher for 0.3 g/cm2
amount cast than for the 0.5 g/cm2
amount
cast. As anticipated, the rate of diffusion is inversely proportional to the thickness (expressed
as a function of the amount cast of acidic chitosan solution). When comparing the two dyes
methylene blue transferred more rapidly than crystal violet.
Table 3. Diffusion rates using Franz cells.
Membrane samples Dye Rate (%/h) R2
Experiment 1
0.3 g/cm2
– 80 DD CV 0.63 0.974
0.5 g/cm2
– 80 DD CV 0.37 0.931
0.3 g/cm2
– 80 DD MB 0.92 0.998
0.5 g/cm2
– 80 DD MB 0.52 0.972
Experiment 2
0.3 g/cm2
– 75 DD MB 1.12 1.000
0.3 g/cm2
– 80 DD MB 1.02 0.999
BioMend absorbable MB 2.53 1.000
Rates are expressed as the percentage of the starting dye material in the upper chamber that diffused into the lower
receptor chamber per hour during the time interval of 1–4 h. CV = crystal violet; MB = methylene blue.
Journal of Biomaterials Science, Polymer Edition 13
14. In another experiment the permeability with regard to methylene blue was compared
between 75 and 80% DD chitosans (Heppe) and relative to a reference bovine collagen mem-
brane – BioMend Absorbable Collagen Membrane®. The flux rate was $2.5Â greater for the
collagen membrane relative to the dense chitosan materials. In addition to having different
chemical compositions between chitosan(s) and collagen with inherent physical/chemical prop-
erties (e.g. potential affinity for the dyes), the chitosan membranes were somewhat thicker (espe-
cially so for the 0.5 g/cm2
amount cast in the first experiment, above) than the collagen material.
Similar attempts to demonstrate passive diffusion with two other dyes, alcian blue (Mw
1299) and Janus Green B (Mw 476), were not successful with the dense chitosan membranes
(data not shown).
In summary, the dense chitosan membranes were permeable in an aqueous environment to
two dissimilar low molecular weight compounds. In addition, permeability was demonstrated
for different amounts of material cast (related to thickness) and for different percentages of
deacetylation (i.e. 80 v. 75% DD). This indicated that at least some small molecules could
pass through the dense chitosan membranes. In other words, the chitosan membranes were
semipermeable.
3.2.2. Enzymatic biodegradation
It is desirable for resorbable membranes to provide a mechanical barrier for weeks to months
in duration during wound healing in vivo, yet be resorbable by enzymatic and/or nonenzy-
matic hydrolysis. Chitosan is subject to enzymatic biodegradation within mammals by lyso-
zyme and other catalytic enzymes. In vitro degradation tests using lysozyme alone showed
the dense chitosan membranes were susceptible to degradation, just as reconstituted collagen
membranes are known to be susceptible to proteases. Compositions with varying % DD were
tested, with the expectation that high deacetylation would have little or no enzymatic degrada-
tion. In vitro results indicated that 75% DD membranes (Fig. 3) and 70% DD membranes
0
20
40
60
80
100
0 5 10 15 20 25 30
80% DD
75% DD
%Degradation
digest period (days)
Figure 3. Lysozyme-mediated degradation of dense chitosan membranes. Dense chitosan membranes
(Heppe 75 & 80% DD, 0.3 g/cm2
cast amount, the standard method) were incubated in lysozyme
solution at 37 °C with daily (M-F) changes of buffer and enzyme. The membranes were imaged at
various times over a period of 3 weeks and densitometry of the membrane translucence was used to
calculate % loss of membrane (75% DD dashed line & squares; 80% DD solid line & circles).
14 T.P. Dooley et al.
15. (data not shown) commenced lysozyme-catalyzed biodegradation relatively quickly within
$4–7 days. However, 80% DD membranes (Fig. 3) exhibited minimal degradation in vitro in
lysozyme solution, as did 85, 90, and 95% DD membranes (not shown).
3.2.3. Nonenzymatic dissolution
At neutral pH, the dense chitosan membranes remain a solid in aqueous solutions. However,
discs of the membranes dissolved in weak acidic environments, similar to the purified chito-
san starting material during manufacturing (i.e. in acetic acid solution). A range of pH levels
was tested below neutrality. Dissolution of 75% DD membranes (Heppe, 0.3 g/cm2
cast
amount, standard method) occurred within 6 h either at pH 2.5 in 1% lactic acid or at pH 3.0
in sodium phosphate buffer, but not at or above pH 3.5. Dissolution of 80% DD membranes
(Heppe, 0.3 g/cm2
cast amount, standard method) occurred within 6 h at pH 2.5 in 1% lactic
acid, but not at pH 3.0 or higher in sodium phosphate buffer. This indicated that the mem-
brane’s carbohydrate polymer was not irreversibly covalently cross-linked during manufactur-
ing. Furthermore, one may feasibly speculate that if the dense chitosan materials were to be
ingested, the harsh acidic pH of the stomach would likely dissolve (depolymerize) the mate-
rial; it would no longer remain in a solid form within the stomach.
3.2.4. Cell culture
Chitosan films have been shown to be biocompatible with human neonatal primary keratino-
cytes and fibroblasts grown in vitro, and regardless of the DD ranging from 53 to 97.5% DD
[47]. Therefore, we anticipated adherence and biocompatibility with our dense chitosan mem-
branes using keratinocytes and fibroblasts. Also included was a third cell type that is relevant
to bone. Human primary keratinocytes and a preosteoblastic cell line adhered to either side of
the dense chitosan membranes and remained viable for the duration of the experiment
(Fig. 4). Similar results were seen with primary human fibroblasts, each cell line proliferating
Keratinocytes Osteoblasts
Down Up Down Up
10x
40x
Figure 4. Dense chitosan membranes (Heppe 80% DD, 0.3 g/cm2
cast amount, the standard method)
were seeded directly with primary human keratinocytes or preosteoblasts and cultured in serum-
containing medium for 3 days at 37 °C. Live cells on the surface of the chitosan membranes were
stained with Cell Tracker Red and imaged by fluorescence microscopy. Duplicate representatives of
each condition are provided. Scale bars: at 10Â magnification = 200 microns and at 40Â
magnification = 50 microns.
Journal of Biomaterials Science, Polymer Edition 15
16. and remaining viable for up to 14 days (not shown). The cellular adhesion and viability were
demonstrated on both planar surfaces of membranes produced by the standard method
(Fig. 4) or the alternative method (data not shown). These results confirmed the in vitro bio-
compatibility and cellular adhesion for three distinct types of human cells, namely keratino-
cytes of the epidermis (ectoderm), fibroblasts of the dermis (mesenchyme), and osteoblasts
(bone).
3.3. In vivo properties
3.3.1. Biocompatibility, resorption, and wound healing
An in vivo study in a rodent animal model showed that implanted dense chitosan membranes
(Primex 79–95% DD by the alternative method) are biocompatible, do not impede healing of
the surgically induced defects, and do not produce adverse tissue reactions. Bilateral full-
thickness 1.5 mm punches were made on the palate of male Lewis rats over the maxillary
ridge and a single dense chitosan membrane was placed at the base of the wound. Responses
to the dense chitosan membranes were determined by clinical observation during the initial
three-day healing period, then by histological analysis of groups of animals at various time
points (1, 3, 6, and 12 weeks).
Clinical re-epithelialization was estimated each day by digital visual inspection as [1 À
(exposed wound area/original wound area) Â 100%]. Wounds with the dense chitosan mem-
brane implanted at their base achieved 80 ± 20% wound closure in the first three days of heal-
ing. Histologically after one week, new epithelium atop the implanted chitosan membrane
demonstrated initial rate peg formation (not shown). These data demonstrated the in vivo bio-
compatibility of the dense chitosan membranes.
With regard to resorption, most of the implants (five of seven) were substantially
degraded (i.e. at least half of the material missing) between 3 and 12 weeks, an example of
which is shown histologically in Fig. 5 (i.e. at six weeks). None of the implants (0 of 2) were
substantially degraded at one week postimplantation (Fig. 5); they remained intact as a barrier
1 week 6 weeks
Chitosan
membrane
Figure 5. Masson’s trichrome stain of rat maxilla after 1 week (left) or 6 weeks (right) of healing with
implanted dense chitosan membranes (Primex 79–95% DD, 0.5 g/cm2
cast amount, the alternative
method). Membranes were placed within full-thickness ulcerative wounds adjacent to the maxilla and
allowed to heal. Note that during resorption the degraded membrane is replaced by a collagenous
interstitial matrix (stained blue). Scale bars = 500 microns.
16 T.P. Dooley et al.
17. membrane. The number of implants that were substantially degraded at the subsequent sam-
pling times were 2 of 2 at 3 weeks, 1 of 2 at 6 weeks, and 2 of 3 at 12 weeks. These results
indicated that the dense chitosan membranes were in fact serving as temporary barrier mem-
branes separating tissue layers for at least one week, and were substantially resorbed in vivo
within 3–12 weeks.
4. Discussion
We were aware of several physical and clinical handling ‘weaknesses’ of resorbable collagen
membranes. Therefore, we desired to develop a biomaterial that demonstrated superiority to
them. Our attention was drawn to purified chitosan as a source material. In the prior art,
chitosan biomaterials were produced by essentially two types of methods: (a) lyophilization
of an acidic chitosan solution, producing a low density ‘sponge’ [48,49] and (b) heated dry-
ing of a chitosan solution without coincident compression, that produces an ‘asymmetric air
dried’ high density material [50,51]. The former lacks sufficient density and strength for use
as a barrier membrane. Although dense, the latter asymmetric air dried material lacks pliabil-
ity, cut-ability, uniformity, and adequate suture-ability for use as a barrier membrane. There-
fore, it would require a new method of manufacturing to produce a dense chitosan barrier
membrane with uniformity and having high clinical utility.
Through a process of reiterative progressive modifications we have developed an innova-
tive manufacturing process to produce high density chitosan membranes that overcome the
weaknesses of both commercial collagen membranes and chitosan materials of the prior art.
The major distinction of this new method was the use of coincident compression-dehydration
of a neutralized polymer of chitosan. To accomplish this, we used a heated vacuum ‘gel
dryer’ to generate uniform pressure and desiccation of the polymeric chitosan inside of a
semipermeable membrane. Additional refinements to the general method included two
approaches to obtaining a dry pliable material that could be easily cut without breaking. The
‘standard method’ employed the inclusion of glycerol prior to compression-dehydration. The
‘alternative method’ lacked the glycerol step, but included an additional final step of hydra-
tion-lyophilization of the dry membrane.
In general, the maximum tensile stress was nearly double in glycerol-containing samples
(standard method) relative to those lacking it and which had undergone the additional step of
hydration/lyophilization. We can speculate as to the reason(s). Glycerol might enhance bind-
ing of polymeric units (e.g. ionic, hydrogen, or Vanderwaal interactions) and/or the alternative
method’s postmanufacturing steps might disrupt binding of polymeric units, perhaps during
the lyophilization step. Some portion of this $2Â differential can be attributed to an
increased average thickness in the hydration/lyophilization samples (i.e. from 0.27 to 0.38 mm
average; Table 2). This differential is also consistent with regard to the averages for the maxi-
mum loads (i.e. 6.2 N with glycerol v. 3.7 N with hydration/lyophilization and lacking glyc-
erol; Table 2). Regardless, a persuasive case can be made for inclusion of glycerol prior to
compression-dehydration. There are multiple benefits of including glycerol during manufactur-
ing, such as ease of cutting, pliability, higher density, lower thickness, and tensile strength
(both max load and max stress).
The dense chitosan membranes produced by the new coincident compression-dehydration
method have sufficient to superior (and in some instances unique) physical, in vitro, and
in vivo properties for use in clinical indications. With regard to physical properties these new
dense chitosan membranes, even when relatively thin ($0.2–0.5 mm), have sufficient flexibil-
ity, tensile strength, elasticity, and resistance to suture pull-out for surgical placement by a cli-
nician. In addition, the tensile strength and suture-ability are correlated with the material’s
Journal of Biomaterials Science, Polymer Edition 17
18. thickness. The material is semipermeable to small molecules, biodegradable using lysozyme,
and biocompatible with human cells in vitro. Furthermore, the chitosan membranes are bio-
compatible with tissues and resorbable in vivo. We anticipate that the rate of resorption can
be ‘controlled’ based upon the percentage of deacetylation and/or thickness of the
membranes, and without requiring a chemical cross-linking agent. Furthermore, the source
material, chitosan, displays inherent antibacterial and antifungal activities. Therefore, our
chitosan-based membranes have this feature as a coincident benefit. This aspect is unique
relative to all other resorbable and nonresorbable commercial surgical membranes.
Our chitosan-based material is fundamentally distinct from the prior art in density coupled
to excellent clinical utility as a surgical barrier membrane. These membranes are entirely
unlike the low density chitosan sponge materials used commercially as hemostatic wound
dressings. It should be noted that chitosan materials have been produced by other processes
that can result in low-to-high density chitosan membranes or films. The two most common
alternatives are compression of a lyophilized acidic chitosan sponge or air-dried asymmetric
chitosan membranes. The former does not produce the superlative densities of the coincident
compression-dehydration method disclosed here. In fact, a commercial lyophilized material is
only about one-tenth of the density of our membranes, is too thick, and transforms rapidly into
a gelatinous form when wet. Thus, this wound dressing lacks the appropriate physical proper-
ties needed for an implantable suture-able membrane. The air-dried asymmetric chitosan mem-
branes can be engineered to have sufficient density. However, they have inferior properties,
such as the lack of pliability, cut-ability, uniformity, and suture-ability. In essence the air-dried
membranes are brittle and not at all suitable for clinical use as a barrier membrane.
The semipermeable nature of the chitosan membranes was demonstrated using methylene
blue and crystal violet. Similar attempts with two other dyes of higher molecular weights,
alcian blue and Janus Green B, were not successful (data not shown). Lack of permeability
might be due to multiple factors, such as increased molecular weights (1299 and 476, respec-
tively), and/or due to affinity for chitosan per se in view of its chemical properties. This
might be due to ionic, hydrophobic, and/or covalent interactions between the dyes and the
chitosan membrane. Regarding ionic interaction, the amino groups of GlcN (i.e. the deacety-
lated moieties) can be protonated and thus have a positive charge. Regarding hydrophobic
interaction, both dyes and the acetylated moieties of chitosan have hydrophobic domains for
association while in an aqueous environment. Therefore, one can speculate on several feasible
explanations for lack of diffusion by these latter two dyes through the membrane into the
aqueous solution of the collection chamber. Regardless, the positive results with methylene
blue and crystal violet demonstrate the material is semipermeable.
The in vitro results indicated that lysozyme as a single enzyme could contribute to the
hydrolysis of high density chitosan membranes, albeit dependent on the % DD. This result
also suggested that one might design different membranes with ‘controlled’ rates of degrada-
tion. A pair of membranes manufactured identically with the sole exception of a low versus
high % DD would be expected to have differential rates of resorption in vivo. For instance,
one can speculate that a 70–80% DD dense chitosan membrane might resorb rapidly (e.g. in
weeks) in a specific surgical implant environment, whereas a 90–95% DD version might
resorb slowly (e.g. in months or longer) in the same environment. Note that in vivo lysozyme
would be only one of several possible degradative pathways toward resorption, and alterna-
tive pathways might synergize (e.g. lysozyme plus NAGase). The rate of resorption could be
selected for the type of surgical indication, based upon the desired thickness and longevity of
the implant. Furthermore, although we have not addressed it in this work, one could possibly
use a chemical cross-linking agent during or following manufacturing to reduce the rate of
resorption of dense chitosan membranes.
18 T.P. Dooley et al.
19. We anticipate that the dense chitosan membranes produced by this new method will have
excellent utility as implantable surgical membranes in oral, orthopedic, general, plastic, and
dermatologic surgeries, among other uses. Examples of oral clinical indications that could
benefit from this new biodegradable material are periodontal ridge augmentation, as well as a
barrier for bone graft substitutes in the mandible or maxilla, and in site preparation prior to
metallic dental implants. We have already demonstrated that the chitosan material is biocom-
patible and resorbable within weeks to months in a rat oral palate model. In orthopedics, one
can envision membrane barriers between bone and soft tissues, for instance as complements
to long bone fractures (especially open fractures) being repaired with hardware and/or bone
graft substitutes. In these cases, the dense chitosan membranes could be sutured, tacked, and/
or stapled in place to prevent in-growth by soft tissue (e.g. fibroblasts). In general surgery,
the membranes could be sutured in situ to provide strength to repair hernias, or to prevent
postsurgical tissue adhesion of visceral organs. In plastic surgery, the dense chitosan mem-
branes could be used to augment soft tissue defects (e.g. facial reconstruction). In dermato-
logic surgery, the membranes could be used as either intact sheets or in mesh form with holes
to promote cutaneous healing following heat or chemical burns, diabetic ulcers, or traumatic
abrasions. In this dermatologic context, the material could serve as either a wound dressing
or a tissue engineering film to facilitate the autologous grafting of normal skin-derived cells
to debrided skin. In conclusion, the surgical indications of use for this new nontoxic resorb-
able biomaterial are myriad.
At present, there are multiple types of resorbable membranes that are commercially avail-
able as US FDA 510(k) cleared Class II devices. The most common are reconstituted bovine
and porcine collagens, although other biomaterials are available commercially (e.g. synthetic
polymers of lactate and/or glycolate and processed xenograft tissues). Our results indicate in
head-to-head comparisons that the dense chitosan membranes have properties meeting and
often exceeding those of commercial collagen membranes. Reconstituted collagen membranes
represent the current standard-of-care in many, if not most, surgical indications. Compared to
the new dense chitosan membranes, the BioMend Absorbable Collagen Membrane® exhibited
lower tensile strength and resistance to suture pull-out. In addition, the clinical handling char-
acteristics of the dense chitosan membranes are superior to that of collagen membranes with
regard to ‘memory’ while wet and the ability to discriminate between the material and sur-
rounding tissues. This is due to collagen rapidly absorbing blood and becoming red in color.
The discrimination between host tissue and the membrane helps to guide placement of the
material and sutures.
Our polysaccharide materials compare favorably to commercial collagen membranes,
and do not have two additional down-side ‘weaknesses’ of collagen membranes. First,
some collagen products require a chemical cross-linker to provide sufficient strength or
resistance to suture pull-out, although it is formally possible that one could use a cross-
linker to enhance our chitosan technology to an even higher level of strength and suture-
ability. Second, there is a perceived concern, especially within the UK and Europe, about
the possibility of inadvertently causing bovine spongiform encephalopathy or ‘mad cow
disease’ using bovine collagen materials, which would not be the case for our chitosan-
based membranes.
Numerous other types of resorbable membranes have been reported, mostly at the pre-
commercial research stage (e.g. electrospun/nanofibrous, laminated, wound dressing, and/or
drug-eluting membranes) [52–55]. In general, although these other types of composite materi-
als can exhibit some physical and biological benefits, they typically lack sufficient resistance
to suture pull-out and strength, unlike the properties exhibited by our dense chitosan mem-
branes (i.e. $1.4 N for suture pull-out and $86 MPa in maximum stress) [53].
Journal of Biomaterials Science, Polymer Edition 19
20. Beyond their potential use in US FDA Class II medical device indications, the dense chito-
san films and membranes could also be used in at least three other broad areas, namely as
wound dressings, delivery devices, and tissue engineering matrices: (1) FDA Class I wound
dressings are used in external and some oral applications. Chitosan ‘sponge’ at low density is
already used for wound dressings, with a mechanistic emphasis on controlling hemorrhage. (2)
Dense chitosan materials could be used as delivery devices for bioactive molecules. For
instance, the chitosan membranes (or postmanufacturing processed granular materials thereof)
are capable of ionic attraction due to protonated amino groups of GlcN residues. It is feasible
that the dense chitosan materials could ‘carry’ nucleic acids, protein and peptide biologics,
pharmaceuticals, vaccines, among other bioactive molecules to promote a physiologic response
in vivo either in or on an animal or human. These controlled delivery devices as combination
products could provide beneficial wound healing, anti-inflammatory, angiogenic, antibiotic, and
other desirable properties. (3) Chitosan films could be used as in vitro scaffolds for tissue engi-
neering purposes. We have already demonstrated that the materials facilitate the adherence and
viability of human keratinocytes, fibroblasts, and osteoblasts. The material could serve in per-
sonalized medicine as an ex vivo bioengineering surface onto which autologous stem cells or
adult cells could be adhered prior to placement on or within the host tissues(s). Variations on
this theme could be used to develop artificial skin or other epithelia (e.g. oral, vaginal, and gas-
trointestinal) prior to surgical attachment at a site of need. The materials should have sufficient
strength, suture-ability, and durability to permit the material to ‘take’ to the host environment.
This type of autologous grafting with a patient’s own cells already adherent to dense chitosan
could provide a more rapid means to wound healing than could be accomplished in the absence
of these therapeutic devices (e.g. in a cutaneous burn or diabetic ulcer environment).
5. Conclusions
The broad array of physical, in vitro, and in vivo testing results indicates that the chitosan
membranes prepared by this method are unlike those produced by any prior art manufacturing
methodology using chitosan as the source material. The dense chitosan membranes have
superior, and in many cases unique, physical, and clinical handling characteristics to those of
a commercial lyophilized acidic chitosan sponge, air-dried asymmetric chitosan membranes,
and a commercial reconstituted collagen membrane. The dense chitosan membranes should
have excellent clinical utility as implantable, resorbable, suture-able surgical membranes that
one anticipates to have inherent anti-infective properties. The suture-able membranes should
provide benefits in wound healing and surgical repair, and can also be designed as coincident
carriers of active ingredients.
Acknowledgments
The project described was supported by Grant Number DE017254 from the National Institute of Dental
and Craniofacial Research through the SBIR program. Its contents are solely the responsibility of the
authors and do not necessarily represent the official views of the NIDCR or the NIH. The innovative
manufacturing processes, composition, and uses of the dense chitosan materials of this work are patent
pending. All rights are reserved by Agenta Biotechnologies, Inc.
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