Comments for diybio, biofiction, vienna, biodesign, mmborchMartin Malthe Borch
Comments and Questions for DIYbio.
Presentation from DIYbio meet-up Vienna Oct. 2014, post Biofiction film festival. About: DIYbio, sociology, science, citizen science, design, living architecture, interaction design, design thinking.
TW Bioart (bioart.tw) is a Bioart community that focuses on stimulating the fields of BioArt and Science+Art in Taiwan, serving as a platform for interactions between biology and art and from which gathers people with interdisciplinary fields. DIYSECT had the great privilege to give a three hour presentation+discussion on the most updated research and documentation of the biohacking practices in the U.S.
Comments for diybio, biofiction, vienna, biodesign, mmborchMartin Malthe Borch
Comments and Questions for DIYbio.
Presentation from DIYbio meet-up Vienna Oct. 2014, post Biofiction film festival. About: DIYbio, sociology, science, citizen science, design, living architecture, interaction design, design thinking.
TW Bioart (bioart.tw) is a Bioart community that focuses on stimulating the fields of BioArt and Science+Art in Taiwan, serving as a platform for interactions between biology and art and from which gathers people with interdisciplinary fields. DIYSECT had the great privilege to give a three hour presentation+discussion on the most updated research and documentation of the biohacking practices in the U.S.
An extensive overview of Hackteria and related project around Open Source Biological Art
1st: my own background and activities bridging science, art and education and how I ended up hunting rabbits in Helsinki.
2nd: Hackerspaces bridging digital- and biotechnologies
3rd: Hackteria, history and projects, NanoSmano, BioTehna, Art/Sci education
4th: HackteriaLab 2014 - Yogyakarta
5th: Background and outlook of urban hunting
dusjagr & nano talk on open tools for agriculture research and learningMarc Dusseiller Dusjagr
Open Tools for Research, Learning, Sharing in Agriculture and
Society as a whole.
Remote lecture by Marc Dusseiller and Fernando "nano" Castro on open science hardware for agriculture and edication, held for students of microbiology at UGM, Yogyakarta (remote - online )
Hackteria BYOP lab introduction of the team and background of why we are looking into "Bring Your Own Poo" as a participatory public laboratory for artistic intervention around privacy of biological data, personal microbiome and shared bacteria in the city.
Part of the P.O.S.T. Hackathon, Seoul, November 2016
thGAP - BAbyss in Moderno!! Transgenic Human Germline Alternatives ProjectMarc Dusseiller Dusjagr
thGAP - Transgenic Human Germline Alternatives Project, presents an evening of input lectures, discussions and a performative workshop on artistic interventions for future scenarios of human genetic and inheritable modifications.
To begin our lecturers, Marc Dusseiller aka "dusjagr" and Rodrigo Martin Iglesias, will give an overview of their transdisciplinary practices, including the history of hackteria, a global network for sharing knowledge to involve artists in hands-on and Do-It-With-Others (DIWO) working with the lifesciences, and reflections on future scenarios from the 8-bit computer games of the 80ies to current real-world endeavous of genetically modifiying the human species.
We will then follow up with discussions and hands-on experiments on working with embryos, ovums, gametes, genetic materials from code to slime, in a creative and playful workshop setup, where all paticipant can collaborate on artistic interventions into the germline of a post-human future.
Natural Aesthetics: Digital Art and Philosophy in the Era of Technologized Bi...Melanie Swan
The arts and technology are coming together in exciting ways in contemporary society. New experimental media such as biology, data, and technology are leading artists, scientists, and other individuals to new realms of knowledge discovery and creative expression. Philosophy, concerned with aesthetics and epistemology (the study of knowledge), provides an interesting lens for understanding current activity in a range of contexts where art, technology, and biology are linked. These contexts include GenerativeArt, BioArt, Biomimicry, Synthetic Biology, and CrowdArt.
on Experimenting with Others. The Rise of D-I-W-O ScienceEri Gentry
on Experimenting with Others. The Rise of D-I-W-O Science.
Profiling hacked biotech equipment (made cheaper and more accessible), world-changing iGEM projects, and online group experiments.
This presentation is on co-creation and was delivered by Max Kortlander at the OpenGovIntelligence propject conference on Nov 22nd 2018 at Delft university of Technology
JRP - Jogja River Project | Ecological Resarch Node of HLab14HLab14
There are three main rivers which pass through the urban areas of Yogyakarta, Code River, Winanga River, and Gajahwng River. Lifepatch in collaboration with many communities have conducted a project called Jogja River Project (JRP) on these rivers since 2011. This project is also part of the Biodesign collaboration with EPFL and will be disseminated in June '14 in Lausanne.
An extensive overview of Hackteria and related project around Open Source Biological Art
1st: my own background and activities bridging science, art and education and how I ended up hunting rabbits in Helsinki.
2nd: Hackerspaces bridging digital- and biotechnologies
3rd: Hackteria, history and projects, NanoSmano, BioTehna, Art/Sci education
4th: HackteriaLab 2014 - Yogyakarta
5th: Background and outlook of urban hunting
dusjagr & nano talk on open tools for agriculture research and learningMarc Dusseiller Dusjagr
Open Tools for Research, Learning, Sharing in Agriculture and
Society as a whole.
Remote lecture by Marc Dusseiller and Fernando "nano" Castro on open science hardware for agriculture and edication, held for students of microbiology at UGM, Yogyakarta (remote - online )
Hackteria BYOP lab introduction of the team and background of why we are looking into "Bring Your Own Poo" as a participatory public laboratory for artistic intervention around privacy of biological data, personal microbiome and shared bacteria in the city.
Part of the P.O.S.T. Hackathon, Seoul, November 2016
thGAP - BAbyss in Moderno!! Transgenic Human Germline Alternatives ProjectMarc Dusseiller Dusjagr
thGAP - Transgenic Human Germline Alternatives Project, presents an evening of input lectures, discussions and a performative workshop on artistic interventions for future scenarios of human genetic and inheritable modifications.
To begin our lecturers, Marc Dusseiller aka "dusjagr" and Rodrigo Martin Iglesias, will give an overview of their transdisciplinary practices, including the history of hackteria, a global network for sharing knowledge to involve artists in hands-on and Do-It-With-Others (DIWO) working with the lifesciences, and reflections on future scenarios from the 8-bit computer games of the 80ies to current real-world endeavous of genetically modifiying the human species.
We will then follow up with discussions and hands-on experiments on working with embryos, ovums, gametes, genetic materials from code to slime, in a creative and playful workshop setup, where all paticipant can collaborate on artistic interventions into the germline of a post-human future.
Natural Aesthetics: Digital Art and Philosophy in the Era of Technologized Bi...Melanie Swan
The arts and technology are coming together in exciting ways in contemporary society. New experimental media such as biology, data, and technology are leading artists, scientists, and other individuals to new realms of knowledge discovery and creative expression. Philosophy, concerned with aesthetics and epistemology (the study of knowledge), provides an interesting lens for understanding current activity in a range of contexts where art, technology, and biology are linked. These contexts include GenerativeArt, BioArt, Biomimicry, Synthetic Biology, and CrowdArt.
on Experimenting with Others. The Rise of D-I-W-O ScienceEri Gentry
on Experimenting with Others. The Rise of D-I-W-O Science.
Profiling hacked biotech equipment (made cheaper and more accessible), world-changing iGEM projects, and online group experiments.
This presentation is on co-creation and was delivered by Max Kortlander at the OpenGovIntelligence propject conference on Nov 22nd 2018 at Delft university of Technology
JRP - Jogja River Project | Ecological Resarch Node of HLab14HLab14
There are three main rivers which pass through the urban areas of Yogyakarta, Code River, Winanga River, and Gajahwng River. Lifepatch in collaboration with many communities have conducted a project called Jogja River Project (JRP) on these rivers since 2011. This project is also part of the Biodesign collaboration with EPFL and will be disseminated in June '14 in Lausanne.
Background about Merapi Biorecovery of Soil | HLab14 - Ecological Research No...HLab14
The project will be based on Lifepatch's ongoing study of agricultural microbiology for soil bio-recovery of soil altered by volcanic eruptions. Based on this field research, novel policy suggestions will be made on how workshops can raise community awareness, and applied to other ecology management issues. Additionally we will try to develop new DIY & Low-Cost Laboratory Infrastructure to enable remote monitoring of the environment up on the volcano mountain.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
1. ● •Breaking up with science - Curious - science being
● •Hacker Culture.
○ •Do’ocracy
○ •Prototypes
● Biologigaragen - DIYBIO:
○ What we do and how.
○ History Maker, hacker community own your code 2005-2010 diybio.or
○ •hobby - Community – strength – Practice.
○ •DiybioMap
○ •Hardware
○ •aesthetics.
○ •Ethics.
● Design:
○ Protocols.
○ Product vs. Prototype
● Citizen Science
○ Biostrike
● Research
○ •Art - A Bioreactor
● Open Innovation
○ •Architecture – living design living.
○ •Design – nice comforting.
○ Baessy / Open source assay tools project
●
●
2. HackteriaLab Symposium 2014
Citizen initiatives in art & science @ UGM
Martin Malthe Borch, @MMBorch
Biologigaragen.org, Labitat.dk, Kopenlab.dk
From diybio to change
Personal stories and reflections
Citizen initiatives in art & science
6. Learn and do science in the same way you
learn and play football? - in social, PASSION DRIVEN clubs-
OPEN to everyone, regardless of your occupation: industry employee,
businessman, politician, bureaucrat, researcher or craftsman
What if you could...
7. The strong association and cooperative-society oriented
culture in Denmark can be a framework for high-tech creative
&
What if ...&
Collaborative Open Innovation?
14. DIY PCR Machine for
Multiplying DNA
OpenPCR
only $600
Commercial
PCR ~ $6000
15. Find Horse in your lasagne Shark in your sushi Who’s your daddy ?!
Join our lab and use it to..
16. Draft DIYbio Code of Ethics from European Congress
Transparency
Emphasize transparency and the sharing of ideas, knowledge, data and results.
Safety
Adopt safe practices.
Open Access
Promote citizen science and decentralized access to biotechnology.
Education
Help educate the public about biotechnology, its benefits and implications.
Modesty
Know you don’t know everything.
Community
Carefully listen to any concerns and questions and respond honestly.
Peaceful Purposes
Biotechnology must only be used for peaceful purposes.
Respect
Respect humans and all living systems.
Responsibility
Recognize the complexity and dynamics of living systems and our responsibility towards them.
Accountability
Remain accountable for your actions and for upholding this code.
36. Biostrike !
A diybio/citizen science experimental competition to find new antibiotics
http://is.gd/Biostrike
Medico-bio project.
Startet in Copenhagen by Malthe & Pieter.
International open collaboration and all over the
world.
Worrkshops in Tel Aviv, Singapore,
Educate - Tech Science and discuss methods currently
used.
Baessy
Open source tools & assays for citizen science.
http://biologigaragen.org/baessy/
37.
38. Copenhagen Citizen Science Center
Organisation and
Surroundings
Successful Spinouts
Knowledge transfer by
Individuals from External
Companies and Universities Open
Associations
Startup Companies &
Work Communities
External Art-tech, diybio &
citizen Science Network
Denmark & Internationally
41. Næringsbiblioteket - The future energy producing waste water treatment plant
1st Prize Winner in collaboration with GXN-3XN Architects, Århusvand Idea Competition
Integrating Biotechnology and Architecture
42. Martin Malthe Borch
Master of Science Biological Engineering
Master in Interaction Design
* Researcher ITU, CGID Bioreactor engineering in Art, Science & Technology
* Integrator of biotech in architecture and design
* Co-founder of Biologigaragen.org #Folkeforskning #CitizenScience
Twitter: @mmborch
Blog hos Ingeniøren: http://ing.dk/blogs/folkeforsker
Billeder: http://www.flickr.com/photos/mmborch/sets/
Linkedin: http://www.linkedin.com/profile/view?id=16826487
www.biologigaragen.org
www.Labitat.dk
