Diazotroph are free living nitrogen fixing bacteria isolated from rhizosphere.

1. ISOLATION AND CHARACTERISTICS OF NOVEL
DIAZOTROPHS FROM VARIOUS REGIONS OF RICE
RHIZOSPHERE
Amit Kumar, Pooja Sharma, Anil Kumar Sharma
MMEC, Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University)-Mullana (Haryana)
SUMMARY
To fulfill the world’s population demand a huge amount of chemical nitrogen is
used for enhancement of crop production. Due to overloaded of chemical
nitrogen or continuous increasing in crop production there is gradually
decreases soil fertility. Chemical fertilizers areso harmful for soil as it causes
soil acidification, water contamination, air pollution and various mysterious
disease that was not found in19th century. Here in present study we have
isolated diazotrophs from rice crop system. For this, total four different regions
(Ambala, Kalaam, Yamuna nagar, Kurukshetra) were selected for soil sample
collections. Using serial dilution method total 300 colonies were isolated from
four different regions.IAA production and Ammonium production test were
performed for characterization of diazotrophs. On the basis of gram staining,
class, size, shape and color, bacterial colonies were further categorized. In
addition to this further characterization of diazotrophs using ammonium
reductase assay is in under progress.This study could be beneficial in future to
characterize the novel diazotrophs.These novel diazotrophs could be a potent
biofertiliser that could replace the havoc effects of chemical fertilizer to sustain
the agricultural land.
METHODOLOGY
About one-third of the agricultural products are produced by
using pesticides and ultimately it harms the human health.
So, it is a big challenge to replace the use of chemical fertilizer
by biological nitrogen.
Diazotrophs, free-living bacteria are estimated to generate an
amount of fixed nitrogen equivalent to almost 60% of the
nitrogen produced industrially via Haber-Bosch.
Diazotrophic bacteria
stress tolerance
enhancement
solubilization and
Mineralization of organic phosphate
inhibition of
plant ethylene
synthesis
Important of Diazotrophs
Why Diazotrophs are a good choice?
RESULT OF AMMONIA AND INDOLE POSITIVE IN VARIOUS REGION
Nitrogen is available in abundance in gaseous form in the
atmosphere but it is unavailable to plants unless it is reduced to
ammonia. The process of reduction of atmospheric nitrogen to
ammonia is carried out by prokaryotic microorganisms was
recorded. Furthermore analysis of isolated diazotrophs using
ARA is in progress. Soon we would characterize the beneficial
diazotrophs to improve the NUE.
CONCLUSION