This document summarizes the detection of swine hepatitis E virus (HEV) in pig livers in Jeju Island, South Korea. Liver samples from pigs showed mild to moderate multifocal hepatitis and portal inflammation. Reverse transcription polymerase chain reaction (RT-PCR) testing detected swine HEV infection in the liver tissue. The conclusions are that swine HEV infection can occur in very young pigs and PCR methods are more sensitive for diagnosing infectious diseases compared to other tests.

1. Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island

2. NonenvelopedFecal-oralrouteClinicalsigns: jaundice, anorexia, nausea, hepatomegalyExertssevereeffectsinpregnantwomenHepatitisE Virus

3. Zoonoticpigsnotobviously ill, displayverymildliverlesions on histopathologicexaminationwidespreadthroughouttheworldAntibodiesto HEV inpigsfromcountrieswhere HEV isendemicand manyindustrializedcountriesPigasanimalreservoir host for HEVHEV inpigs

4. Animalsamples

5. All liversampleswerecollectedfrompigs at necropsyOne-thirdfrozen at -70℃ remainingpart fixedin 10% neutralbufferedformalinembeddedinparaffinwaxcutinto 3 µmsectionsstainedwithhematoxylin and eosinTissueprocessing

6. RT-PCR findings

7. Moderate to severe multifocal lymphoplasmacytic and histiocytic hepatitis or portal inflammation

8. mild hepatic enlargement and scattered yellowish discoloration foci

9. focal infiltration of lymphocytes, plasma cells, and macrophages in hepatic sinusoids and the portal triad

10. swineHEV infectionoccurs at a veryyoungage (under 2 months) inJejupigsvarioushepatitis virus antigensdetectedinlivertissueviral markersmaynotbe present duringactivediseaseimmunohistochemistrynotparticularlyusefulPCR methodmoresensitive and applicableas a specificassayforthediagnosisofvariousinfectiousdiseasesindividualswithswine-associatedoccupations at possible risk ofzoonoticinfection.Conclusions