This study evaluated the crude extract of the mushroom Asterius hygrometricus as an anticancer agent against cervical cancer cells (HeLa cell line). The crude extract was tested at various micromolar concentrations and found to inhibit the growth of HeLa cells in a dose-dependent manner, with IC50 values of 60.25±1.201mg/ml and 57.12±0.15mg/ml for water and methanol extracts respectively. Cell viability assays using PI and DAPI staining under fluorescence microscopy also showed the extract inhibited cervical cancer cells. The results suggest the Asterius hygrometricus extract may be a promising and low-cost treatment for human cervical cancer.