1. Curriculum Vitae
Justin C. Villarreal
5727 Firenza Dr
Houston Texas 77035
Phone: 956-533-6621
Email: Jvillarreal1011@gmail.com
Experience/Qualifications
-Molecular Diagnostics Laboratory MD Anderson Cancer Center: June 2012-Current
 Database Coordinator
o Lab liaison between the IT and DBC team and the lab
o Basic knowledge of SQL server
o Microsoft Access database management skills
 Lead LIMS software developer and Trainer
o Lead developer for Cerner Millennium build for the Molecular
Diagnostic Lab
o Lead Developer and Builder for Next Generation Clarity LIMS
software
o Lead trainer for all 95 MDL Lab and Clerical Personnel for both
projects
o SME for the Molecular lab for all LIMS projects in the lab
 Other Duties
o Trainer/Mentor for new employees and fellows
o Quality Control/Inventory Control Management
o Laboratory Instrument Maintenance
o Cost Analysis for new assay development.
o Leader of the extraction and distribution of MD Anderson's
Moonshot Project
 Proficient in Microsoft Word, Excel, and Power Point, Visio, bilingual
(Spanish)
 Automation Skills
o Clinical development and validation of Maxwell CSC for DNA
and RNA extraction on Blood and Bone Marrow samples
o Validation of the Maxwell 16 MDX for Saliva, Buccal Swab, BM
and Blood for clinical uses
o Validation of the Qiacube for Epstein-Barr Virus
o Validation of the Roche Cobas for CMV extraction
o Evaluation/Validation of Promega Reliaprep for automated large
volume DNA extraction from Blood and Bone Marrow
o Validation of RoboSep Nimbus for large volume T and Myeloid cell
sorting
o Proficient in Tecan troubleshooting for both open platforms and
the Promega Reliaprep system.
o Proficient in troubleshooting Hamilton RoboSep Nimbus
o Qiasymphony troubleshooting
o Hamilton Star basic troubleshooting knowledge
o Evaluated multiple DNA/RNA extraction platforms when the lab
was beginning the move into automation
 New Assay Development

2. o Validation of two cancer mutation screens for clinical diagnosis:
Cancer Mutation Screen for 28 genes whole exon and a 53
gene hot spot panel for leukemia patients
o Validation of Haloplex vs. Truseq Chemistry for both the 53 and
28 gene custom panels
o Validation of the MiSeq 2500 for use with Illumina TruSeq
Chemistry for clinical diagnostic samples to increase sample
volume and streamline workflow
o Validation of NextGene 3rd party software for SNP variant
calling for the diagnosis of cancer
 Molecular Diagnostic Skills
o DNA/RNA Extraction on various platforms
o Next Generation Sequencing on Illumina and Ion Torrent
o Antibody Cell Sorting for T&B cells
o Sanger Sequencing
- Research Assistant University of Texas Medical School: August 2011-June 2012
 Performed Nucleic acid purification
 Bacterial Cloning
 Real-Time PCR
 Transfection/Transduction Studies
- National Donor Testing Services Gulf Coast Regional Blood Bank: August 2009-
April 2010
 Performed ELISA testing for Hepatitis A, B, and C diagnosis
 Performed Multiplex PCR Assay using a Roche system for the diagnosis
of 14 blood borne pathogens.
- Student Researcher Dr. Francisco Vega-Vasquez UT MD Anderson: August
2008- August 2009
 Using Fluorescent In Situ Hybridization I studied the role of the SHH/GLI1
signaling pathway in DLBCL
 Performed PCR, Real time PCR, NICK translation, and FISH using home
brew probes
 Presented research at two national conferences: Sigma Xi (Orlando) and
Annual Biomedical Research Conference for Minority Students (ABRCMS)
(Austin ,TX)
- MB(ASCP)CM
Certified License # 25314370 (August 2009-Current)
EDUCATION
- University of Texas at M.D. Anderson Cancer Center, Houston, TX
 Bachelor of Science in Molecular Genetic Technologist: August 2009
– August 2010
 GPA 4.0/4.0
- Texas A&M University Corpus Christi
 Bachelor of Science in Biomedical Sciences: August 2004 - May 2008
 Graduated cum laude, with Honors designation
 Course work in Biochemistry, Chemistry, Biology, Histology, Virology,
Immunology, Microbiology

3. EMPLOYMENT
- Molecular Genetic Technologist/Data Base Coordinator: MDACC, Houston,
TX: June 2012- Present
- Collection Specialist: Penitentiary Mobile DNA, Houston, TX: August 2008 –
August 2009
- Molecular Genetic Technologist: GCRBB, Houston, TX: August 2009 - April
2010
- Research Assistant: Texas A&M, Corpus Christi, TX: August 2004 - July
2008
PROFESSIONAL ASSOCIATIONS
- Association for Molecular Pathology Feb 2012 – Present
- Association of Genetic Technologists Jan 2008 – Present
- American Society for Clinical Laboratory Sciences August 2009 – Present