it on cloning and gene editing

2. Cloning
and
Gene Editing
MYP
Disha Chatterjee

3. You can enter a subtitle here if you need it

4. You can enter a subtitle here if you need it

5. Can you identify this duo?
Ian Wilmut with his Dolly, 5th July 1996

7. Definition
CLONING

8. Can cloning occur naturally?
Identical twins Asexual Reproduction

9. How is cloning different from sexual reproduction?
Sexual Reproduction combines two
gamaetes for producing a zygote whereas
cloning produces an exact copy of the
individual.
Sexual Reproduction
In fertilization, the
sperm and egg have one
set of chromosomes each.
When the sperm and egg
join, they grow into an
embryo with two sets—one
from the father's sperm
and one from the
mother's eggs.
SCNT -Somatic Cell
Nuclear Transfer
In SCNT, the egg cell's
single set of chromosomes
is removed. It is replaced
by the nucleus from a
somatic cell, which already
contains two complete sets
of chromosomes. So, in the
resulting embryo, both sets
of chromosomes come
from the somatic cell.

10. Dolly the sheep, the world's
first cloned mammal, is
announced to the world and
meets the media, at Roslin
Institute, in Roslin, Scotland,
on 22nd February 1997.

12. The process

15. Other types of cloning- Gene cloning
Steps in Gene Cloning
Basic 7 steps are:
● Isolation of DNA [gene of interest] fragments
to be cloned.
● Insertion of isolated DNA into a suitable
vector to form recombinant DNA.
● Introduction of recombinant DNA into a
suitable organism known as host.
● Selection of transformed host cells and
identification of the clone containing the gene
of interest.
● Expression of the introduced Gene in the
host.
● Isolation of multiple gene copies/Protein
expressed by the gene.
● Purification of the isolated gene copy/protein
Gene Cloning
Study of viruses
03
Vaccines
02
Prsa
Used in large scale
protein production
01
Practised widely in molecular
biology labs since 1970.
No controversies in this branch
of cloning

16. Therapeutic cloning

18. A question…..
Do you think government should rethink on the laws
regarding cloning?

20. Despite being red, Mars is
actually a cold place. It’s full of
iron oxide dust
Venus has a very beautiful
name and is the second planet
from the Sun
Mercury is the closest planet to
the Sun and the smallest one in
the Solar System
Neptune is the fourth-largest
planet in the Solar System.
Neptune is an ice giant
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