This document introduces colligative properties, which describe how adding a solute to a solution affects the solution's boiling point, freezing point, and vapor pressure. There are three key rules: 1) the solute must dissolve in water, 2) the specific identity of the solute does not matter, only the amount, and 3) replacing about half the water with a soluble substance lowers the freezing point. Common household items like sugar, salt, and alcohol exhibit colligative properties and can be used in place of antifreeze to prevent water in a car from freezing. The mechanism is that solute molecules get in the way of ordering processes like freezing and boiling, increasing the entropy and energy needed for phase changes
Here is a quick handout on water purification. Water is life and hopefully this will be useful in studying how to obtain it and make it safe for drinking.
Water for Living and Survival. What You Need To KnowBob Mayer
Drinkable Water is almost always your #1 survival priority. How much do you need? How much should you have on hand? How can you purify water? What gear should you get?
The Most Precious and Important Survival ResourceBob Mayer
Water for Living and Survival. What You Need To Know. Drinkable Water is almost always your #1 survival priority. How much do you need? How much should you have on hand? How can you purify water? What gear should you get?
Winter ecology is like all life - it begins with chemical interactions and finally with energy expenditures.
Here are some background details to use in exploring this aspect.
Water for Living and Survival. What You Need To KnowBob Mayer
Future wars will be fought over war; more so than oil. Drinkable Water is almost always your #1 survival priority. How much do you need? How much should you have on hand? How can you purify water? What gear should you get?
Here is a quick handout on water purification. Water is life and hopefully this will be useful in studying how to obtain it and make it safe for drinking.
Water for Living and Survival. What You Need To KnowBob Mayer
Drinkable Water is almost always your #1 survival priority. How much do you need? How much should you have on hand? How can you purify water? What gear should you get?
The Most Precious and Important Survival ResourceBob Mayer
Water for Living and Survival. What You Need To Know. Drinkable Water is almost always your #1 survival priority. How much do you need? How much should you have on hand? How can you purify water? What gear should you get?
Winter ecology is like all life - it begins with chemical interactions and finally with energy expenditures.
Here are some background details to use in exploring this aspect.
Water for Living and Survival. What You Need To KnowBob Mayer
Future wars will be fought over war; more so than oil. Drinkable Water is almost always your #1 survival priority. How much do you need? How much should you have on hand? How can you purify water? What gear should you get?
Water for Living and Survival. What You Need To KnowBob Mayer
Drinkable Water is almost always your #1 survival priority. How much do you need? How much should you have on hand? How can you purify water? What gear should you get?
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Water for Living and Survival. What You Need To KnowBob Mayer
Drinkable Water is almost always your #1 survival priority. How much do you need? How much should you have on hand? How can you purify water? What gear should you get?
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
2. An ice storm is coming. Your car’s radiator has no
antifreeze in it; all stores are closed, and you’ve
got to use whatever you have around the house to
save your car’s engine.
Also remember the motto for
Colligative Properties
Getting in the way is Good,
Creating Disorder is Better
It would be ideal to have antifreeze
(ethylene glycol) but remember it’s
quantity not quality that counts here.
3. The next morning you wonder if what you did
saved you thousands of dollars on a new engine.
Rule one: What you add has to dissolve in water.
Rule two: If you add a solid that dissolves in
water, it doesn’t matter what it is, just the amount.
Rule three: Just like antifreeze, your goal is to
replace about ½ of the water with a solid or liquid
that is miscible with water.
Examples 1: Sugar, salt, baking soda, shampoo,
laundry detergent, pancake syrup.
Examples 2: Rubbing alcohol, brake fluid
4. Again, it seems odd that the chemical nature of these
different compounds are not a factor in regards to how
much they depress the freezing point.
Examples 1: Sugar, salt, baking soda, shampoo,
laundry detergent, pancake syrup.
It reminds us of the ideal gas law: PV=nRT
The properties are not affected by what kind of
gas we are talking about (at least in situations
where they are behaving as an ideal gas).
5. How does it work?
1)Get in the way.
2)Create Disorder
Reason 1: As the water tries to freeze,
the other molecules get in the way.
Reason 2: By adding these other substances,
you’ve added disorder to the mixture. Nature
tends to favor disorder (entropy). When water
tries to freeze, it has to get organized, which will
take more energy.
Freezing point
depression
6. This frog is frozen, but the water in it did not turn
into ice crystals, which would have ruptured the
cells in its body. Why didn’t ice crystals form?
Glucose and glycerol in its blood and cells
prevent water from freezing.
8. This graphic helps us see the randomness that mixing causes. As a mixture, the
liquid that would normally freeze at a higher temperature is prevented from freezing
by the other liquid or solid. Again the cause is the solute gets in the way and the
freezing as to reverse the randomness (entropy) which takes more energy.
9. Antifreeze is often called
“coolant” because it not
only can lower the freezing
point of water, it can
elevate the boiling point of
water.
So, if you don’t have any
antifreeze (coolant), what
could you use instead?
Yes, the same items that
you picked to keep it from
freezing.
10. Notice that ice cream melts differently
than ice. Ice stays hard until it melts.
Ice cream gradually get softer and
softer.
11. Ice is a pure substance but ice
cream is a mixture. In other
words, there are other
chemicals that get in the way
of water freezing.
So you have get
colder than 0°C
to get it to
freeze.
About 30% of the water in
ice cream never freezes
because of the high level
of dissolved solids like
sugar, fats, and proteins.
14. Pure molten silica (SiO2)
freezes into quartz at
around 2000°C, but if
mixed with CaO and
Na2CO3, it freezes at
about 1000°C. Other
additives can bring it
down to 500°C. Actually,
at room temperature it is
still not completely frozen.
Glass is classified as a
supercooled liquid. Over
many millenia, this
drinking glass will slowly
“melt” into a pool of glass.
15. Eggs are mostly
water, but
dissolved
proteins keep
them from
freezing at 0°C.
Chefs take
advantage of this
in frozen
desserts.
16. How do you make red blood cells go from this shape to this?
Get in the way
And quantity not quality is all that counts.
18. Cell Membrane
Inside cell
Outside cell (hypertonic)
Na
Cl
Cl
Cl
Cl
Na
Na
Na
Na
Na
Eventually an equal number will
pass back and forth, but only after
the outside has a greater share of
the water molecules.
Anything soluble will do the same
thing (quantity not quality) For
example, any used for freezing
point depression would cause
this.
The extra solute (e.g., NaCl) gets in
the way of the outside water passing
through the membrane. Note:
Solutes cannot pass through membrane.
19. Vapor pressure is reduced by the addition of a non-
volatile solute for the same reasons as freezing point
depression and boiling point elevation.