Enzymes are important chemical compounds that speed up reactions in organisms and lower the energy required. The document discusses an experiment to test biofuel samples using a spectrometer. A standard calibration curve was made and used to test biofuel samples and calculate the amount of p-Nitrophenol produced at different temperatures. The results showed no p-Nitrophenol was produced at 0°C, about 75 nmol was produced at 22°C, and around 150 nmol was produced at 37°C.