Metal-organic molybdenum complexes were synthesized by the hydrothermal method using ammonium heptamolybdate as the metallic source, and as the organic ligand terephthalic acid (BDC) or bis(2-hydroxyethyl) terephthalate (BHET), obtained via glycolysis of poly(ethylene)terephthalate (PET). The BDC-Mo and BHET-Mo complexes were characterized by XRD, N2 physisorption, TGA, ATR-FTIR, SEM, XPS and their in vitro biocompatibility was tested by porcine fibroblasts viability. The results show that molybdates (MoO4-2) are coordinated to the carbonyl functional groups of BDC and BHET by urea bonding (-NH-CO-NH-) which is related to their high biocompatibility and high thermal stability. These organic molybdate complexes possess rectangular prism particles made up of rods arrays characteristics of molybdenum oxides (MoO3). The organic complexes BDC-Mo and BHET-Mo do not show to be cytotoxic for porcine dermal fibroblasts growing on their surface for up to 48 h of culture.
Incorporation of Se (IV) Complexes based on Amino Acids in Biomatrixes in Hyd...IIJSRJournal
Selenium is a non-metal that shows biological interest since it is responsible for modulating various proteins at the micronutrient level in living beings. In this work, new complexes based on the Se (IV) ion with amino acids such as phenylalanine (Se-F), histidine (Se-H) and tryptophan (Se-T) were hydrothermally synthesized and characterized. These were incorporated into biomatrixes based on semi-interpenetrated polymeric networks (Semi-IPN) of collagen-polyurethane-guar gum (CPGG) by the microemulsion process using a mass ratio of 1 wt.% with respect to collagen. The structural and crystalline characteristics that the selenium-amino acid complexes show a performance in modulating the properties of the biomatrixes under study. The results indicate that the incorporation of the complex decreases the crosslinking of the hydrogel, generating granular surfaces with porosity dependent on the type of amino acid. The CPGG Se-T biomatrix shows a swelling capacity of 10200 ± 1100 higher than the CPGG base matrix; while the CPGG Se-F and CPGG Se-T biomatrixes present slow degradation at both physiological and acidic pH. Interestingly, the matrix that includes the Se-F complex significantly stimulates the metabolic activity of L929 fibroblasts for up to 48 h, stimulating their proliferation. The fibroblasts encapsulated on these novel biomatrixes show recurrent release capacity for up to 7 days, where the structure of the CPGG Se-H biomatrix exhibits greater release from the encapsulated cells. These results demonstrate that these innovative biomatrixes could be used in biomedical applications such as dermal tissue regeneration and cell release for a specific biological fate.
Green synthesis of iron-organic framework Fe-BTC using direct ultrasound to r...IJAEMSJORNAL
Metal-organic framework materials (MOFs) comprise organic bridges and metal centers (as connection points). MOFs have unique properties such as crystal structure, large specific surface area, flexible structural framework, and can change the size and shape of pores and diverse chemical functional groups inside the pores. In this study, metal-organic framework materials based on iron (Fe) and the organic ligand H3BTC were successfully synthesized by ultrasonic method and evaluated for their ability to remove MB dye through Investigate the effects of MB concentration, pH, isotherm model, and adsorption kinetics. With a size of about 100 - 200 nm and an excitation wavelength in the ultraviolet region, the synthesized material shows potential in environmental treatment when the adsorption efficiency reaches over 60% after just 10 minutes and over 80% both processes under sunlight conditions. In addition, the synthesized material is also evaluated to have selective adsorption with Methylene Blue dye.
On the Modulation of Biocompatibility of Hydrogels with Collagen and Guar Gum...IIJSRJournal
In this work, we report the synthesis of molybdenum metal-organic frameworks (Mo-MOFs) using 1,3,5-benzenetricarboxylic acid and the amino acids L-phenylalanine, L- tryptophan, and L-histidine as ligands. They were incorporated in hydrogel matrixes comprised of collagen and guar gum to obtain composite hydrogels. The effect of chemical structure of Mo-MOFs on the structure, physicochemical properties and in vitro biocompatibility of hydrogels was studied. These biomaterials showed a super absorbent performance (higher than 2000 ± 169%) and a high degree of reticulation (higher than 75 ± 6%). The microstructure of the composites showed a granular morphology with some porosity. These composites were degraded entirely by hydrolysis at pH 5 and pH 7 at room temperature in time lapses shorter than 15 days. Also, they were biocompatible with porcine dermis fibroblasts not showing cytotoxic effects up to 48 h of incubation allowing its proliferation, and it was observed that the MOF containing L-tryptophan improved notably the biocompatibility of the collagen/guar gum matrix. Finally, the matrixes were tested as vehicles for cell encapsulation and release. The slow-release rates show that fibroblasts tend to remain inside the hydrogel matrixes. Thus, these materials are more suitable for cell scaffolds and tissue engineering applications such as wound healing dressings.
Incorporation of Se (IV) Complexes based on Amino Acids in Biomatrixes in Hyd...IIJSRJournal
Selenium is a non-metal that shows biological interest since it is responsible for modulating various proteins at the micronutrient level in living beings. In this work, new complexes based on the Se (IV) ion with amino acids such as phenylalanine (Se-F), histidine (Se-H) and tryptophan (Se-T) were hydrothermally synthesized and characterized. These were incorporated into biomatrixes based on semi-interpenetrated polymeric networks (Semi-IPN) of collagen-polyurethane-guar gum (CPGG) by the microemulsion process using a mass ratio of 1 wt.% with respect to collagen. The structural and crystalline characteristics that the selenium-amino acid complexes show a performance in modulating the properties of the biomatrixes under study. The results indicate that the incorporation of the complex decreases the crosslinking of the hydrogel, generating granular surfaces with porosity dependent on the type of amino acid. The CPGG Se-T biomatrix shows a swelling capacity of 10200 ± 1100 higher than the CPGG base matrix; while the CPGG Se-F and CPGG Se-T biomatrixes present slow degradation at both physiological and acidic pH. Interestingly, the matrix that includes the Se-F complex significantly stimulates the metabolic activity of L929 fibroblasts for up to 48 h, stimulating their proliferation. The fibroblasts encapsulated on these novel biomatrixes show recurrent release capacity for up to 7 days, where the structure of the CPGG Se-H biomatrix exhibits greater release from the encapsulated cells. These results demonstrate that these innovative biomatrixes could be used in biomedical applications such as dermal tissue regeneration and cell release for a specific biological fate.
Green synthesis of iron-organic framework Fe-BTC using direct ultrasound to r...IJAEMSJORNAL
Metal-organic framework materials (MOFs) comprise organic bridges and metal centers (as connection points). MOFs have unique properties such as crystal structure, large specific surface area, flexible structural framework, and can change the size and shape of pores and diverse chemical functional groups inside the pores. In this study, metal-organic framework materials based on iron (Fe) and the organic ligand H3BTC were successfully synthesized by ultrasonic method and evaluated for their ability to remove MB dye through Investigate the effects of MB concentration, pH, isotherm model, and adsorption kinetics. With a size of about 100 - 200 nm and an excitation wavelength in the ultraviolet region, the synthesized material shows potential in environmental treatment when the adsorption efficiency reaches over 60% after just 10 minutes and over 80% both processes under sunlight conditions. In addition, the synthesized material is also evaluated to have selective adsorption with Methylene Blue dye.
On the Modulation of Biocompatibility of Hydrogels with Collagen and Guar Gum...IIJSRJournal
In this work, we report the synthesis of molybdenum metal-organic frameworks (Mo-MOFs) using 1,3,5-benzenetricarboxylic acid and the amino acids L-phenylalanine, L- tryptophan, and L-histidine as ligands. They were incorporated in hydrogel matrixes comprised of collagen and guar gum to obtain composite hydrogels. The effect of chemical structure of Mo-MOFs on the structure, physicochemical properties and in vitro biocompatibility of hydrogels was studied. These biomaterials showed a super absorbent performance (higher than 2000 ± 169%) and a high degree of reticulation (higher than 75 ± 6%). The microstructure of the composites showed a granular morphology with some porosity. These composites were degraded entirely by hydrolysis at pH 5 and pH 7 at room temperature in time lapses shorter than 15 days. Also, they were biocompatible with porcine dermis fibroblasts not showing cytotoxic effects up to 48 h of incubation allowing its proliferation, and it was observed that the MOF containing L-tryptophan improved notably the biocompatibility of the collagen/guar gum matrix. Finally, the matrixes were tested as vehicles for cell encapsulation and release. The slow-release rates show that fibroblasts tend to remain inside the hydrogel matrixes. Thus, these materials are more suitable for cell scaffolds and tissue engineering applications such as wound healing dressings.
Impact of carbon nanomaterials on the formation of multicellular spheroids by...Татьяна Гергелюк
This paper investigates the effect of different concentrations of nanostructured materials: fullerene-like (С60), onion-like carbon
(OLC) and ultra dispersed diamonds (UDD) on the formation of multicellular spheroids (MS). Chemical composition and purity of
nanomaterials is controlled by Fourier transform infrared spectroscopy (FT-IR). The strength and direction of the impact of
nanomaterials on the cell population was assessed using microphotography of multicellular spheroids culture and Pearson's correlation
coefficient. The results demonstrated that UDD and OLC reduced adhesion and cohesive ability of cells and stimulated generation of cell
spheroids of ~ 3 ∙ 10 mm3 in significant amount. The fullerenes reduced in the main cell adhesion to substrate that led to formation of
cell aggregates of ~ 5 ∙ 10-3 mm3. The results could be useful for achievement of the directed cell
Impact of carbon nanomaterials on the formation of multicellular spheroids by...Татьяна Гергелюк
This paper investigates the effect of different concentrations of nanostructured materials: fullerene-like (С60), onion-like carbon (OLC) and ultra dispersed diamonds (UDD) on the formation of multicellular spheroids (MS). Chemical composition and purity of nanomaterials is controlled by Fourier transform infrared spectroscopy (FT-IR). The strength and direction of the impact of nanomaterials on the cell population was assessed using microphotography of multicellular spheroids culture and Pearson's correlation coefficient. The results demonstrated that UDD and OLC reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids of ~ 3 ∙ 10 mm3 in significant amount. The fullerenes reduced in the main cell adhesion to substrate that led to formation of cell aggregates of ~ 5 ∙ 10-3 mm3. The results could be useful for achievement of the directed cell growth in three-dimensional culture.
— Processes based on immobilized enzymes have been studied extensively in the last few decades and today are also applied to the safeguard of environmental parameters. In this work, zeolite composite flat membranes with different chemical composition, transition metal, and microporous structures were prepared using in situ and secondary growth crystallization synthesis methods in/on stainless steel porous disks. All zeolite materials were been used in catalase adsorption to analyze the zeolite behavior andthe effect of chemical composition and structure on interaction with the enzyme. This study shows that the electrostatic type of interaction seems to be of the utmost importance in influencing immobilization, while the zeolite Brönsted acidity of the support is the subordinate parameter, which differentiates the adsorption performances of different zeolite structures (that distinct for chemical composition of the framework). Moreover, it permits to conclude that transition metal-containing membranes adsorb a higher percentage of the enzyme with respect to no-exchanged membranes and that, for all materials synthesized, the amount of catalase adsorbed onto the zeolite crystals and membranes increases with the temperature.
Micellar Effect On Dephosphorylation Of Bis-4-Chloro-3,5-Dimethylphenylphosph...IOSR Journals
The rate enhancement depends on the hydrophobicity of the nucleophile. The micellar catalyzed reaction between bis-4-chloro-3,5-dimethylphenylphosphate ester and hydroxide or hydroperoxide anions has been examined in buffered medium (pH 8-10). First order rate constant (Kψ) for the reaction of hydroxide ion with bis-4-CDMPP go through maxima with the increasing concentration of cetyltrimethylammoniumbromide (CTABr). Micelles of CTABr very effective catalyst to the reactions of phosphate diesters. Rate constants measured with OH2- ions are approximately twice and thrice than that of OH- ions in presence of CTABr.
Micellar Effect On Dephosphorylation Of Bis-4-Chloro-3,5-Dimethylphenylphosph...IOSR Journals
The rate enhancement depends on the hydrophobicity of the nucleophile. The micellar catalyzed reaction between bis-4-chloro-3,5-dimethylphenylphosphate ester and hydroxide or hydroperoxide anions has been examined in buffered medium (pH 8-10). First order rate constant (Kψ) for the reaction of hydroxide ion with bis-4-CDMPP go through maxima with the increasing concentration of cetyltrimethylammoniumbromide (CTABr). Micelles of CTABr very effective catalyst to the reactions of phosphate diesters. Rate constants measured with OH2- ions are approximately twice and thrice than that of OH- ions in presence of CTABr.
Fibrous Scaffold Produced By Rotary Jet Spinning TechniqueIJERA Editor
Poly(L-lactic acid) (PLLA)/ poly(ɛ-caprolactone) (PCL) mesh was produced by Rotary Jet Spinning (RJS)
process. RJS is a simple method which fabricates three-dimensional fibers by exploiting a high-speed rotating
nozzle o form a polymer jet which undergoes stretching before solidification without the need of high voltage.
Blend meshes were characterized by scanning electron microscopy (SEM), thermo gravimetric analysis (TGA),
differential scanning calorimeter (DSC) and infrared spectroscopy Fourier transform (FTIR). SEM imagens
provides information about the morphological structure, which confirmed the production of fibers using RJS.
Data obtained by thermal analyzes indicated the immiscible property of PLLA/PCL blend and also the total
solvent evaporation. As a preliminary in vitro assay it was investigated using Vero cells, was not found any sign
suggesting cell toxicity, indicating biocompatibility. Thus, this report suggests the use of PCL/PLLA mesh as
fiber scaffold substrate for tissue engineering
Biocompatibility of Poly (L-Lactic Acid) Synthesized In Polymerization Unit B...IJERA Editor
The absorbable polyacid is one of the most used and studied materials in tissue engineering. This work
synthesized a poly (L-lactic acid) (PLLA) through ring-opening polymerization and produced nanofibers by the
electrospinning process. The PLLA was analyzed by FTIR and the cytotoxicity was evaluated by the MTT assay
and Live/Dead®. The hemocompatibility was tested by platelet adhesion and hemolytic activity assay. The tests
were performed in contact with human mesenchymal cells at varying times. The high rates of cell viability and
proliferation shown by MTT and Live/Dead® tests demonstrate that this PLLA is a non-toxic material and the
hemocompatibility assay revealed that the biomaterial was also biocompatible. It was achieved as well the
successful production of electrospinning nanofibers, which can be converted for specific biomedical applications
in the future
In the early twentieth century, major representatives of the Jadid movement became active participants in the socio-political processes in the Turkestan region. Usmonkhoja Polatkhoja, a progressive from Bukhara, was one of the beams not only in the Emirate of Bukhara, but also in Turkestan. He first participated in the reforms and progressives, and later in the national liberation movements, and fought for the prosperity and independence of the country.This article provides information about Usmonkhoja's life and work in Jadidism, revolts, national liberation struggles, and emmigiration.
Flood is one of the natural disaster known to be part of the earth biophysical processes, which its occurrence can be devastating; due to mostly anthropogenic activities and climatological factors. The aim of the research is to identify and map the extent at which the impact of flood due to intense rainfall and rise in water in the study area using geospatial techniques and the specific objectives are to carry out terrain analysis of the study area and to generate flood indicator maps of the study area. The study analyzed rain fall data;, the drainage system and Shuttle Radar Topographic Mission (SRTM 30m) of the area. ArcGIS 10.8 was to modelled and to generate the contributing factors map of the study area. The drainage system was generated through on-screen digitization of topographic map of scale 1:50,000 of Ondo South-West. The mean annual rainfall of Lagos State was generated in the ArcGIS environment from the rainfall data through spatial analysis tool. The SRTM was used in terrain analysis of the study area. The results generated showed the lowest mean annual rain fall of the area 1,700mm and the highest mean annual rain fall was 2,440mm. Digital elevation model (DEM), slope, flow direction were generated from the SRTM. Drainage density of the area was generated using the drainage system. The slope map of the entire area which are classified into five slope classes of very high (14%-48.5%) to high (7.6%-13.9%) to moderately high (4.2%-7.6%) to low (1.5%-4.2%) and very low (0. % - 1.2%).
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Impact of carbon nanomaterials on the formation of multicellular spheroids by...Татьяна Гергелюк
This paper investigates the effect of different concentrations of nanostructured materials: fullerene-like (С60), onion-like carbon
(OLC) and ultra dispersed diamonds (UDD) on the formation of multicellular spheroids (MS). Chemical composition and purity of
nanomaterials is controlled by Fourier transform infrared spectroscopy (FT-IR). The strength and direction of the impact of
nanomaterials on the cell population was assessed using microphotography of multicellular spheroids culture and Pearson's correlation
coefficient. The results demonstrated that UDD and OLC reduced adhesion and cohesive ability of cells and stimulated generation of cell
spheroids of ~ 3 ∙ 10 mm3 in significant amount. The fullerenes reduced in the main cell adhesion to substrate that led to formation of
cell aggregates of ~ 5 ∙ 10-3 mm3. The results could be useful for achievement of the directed cell
Impact of carbon nanomaterials on the formation of multicellular spheroids by...Татьяна Гергелюк
This paper investigates the effect of different concentrations of nanostructured materials: fullerene-like (С60), onion-like carbon (OLC) and ultra dispersed diamonds (UDD) on the formation of multicellular spheroids (MS). Chemical composition and purity of nanomaterials is controlled by Fourier transform infrared spectroscopy (FT-IR). The strength and direction of the impact of nanomaterials on the cell population was assessed using microphotography of multicellular spheroids culture and Pearson's correlation coefficient. The results demonstrated that UDD and OLC reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids of ~ 3 ∙ 10 mm3 in significant amount. The fullerenes reduced in the main cell adhesion to substrate that led to formation of cell aggregates of ~ 5 ∙ 10-3 mm3. The results could be useful for achievement of the directed cell growth in three-dimensional culture.
— Processes based on immobilized enzymes have been studied extensively in the last few decades and today are also applied to the safeguard of environmental parameters. In this work, zeolite composite flat membranes with different chemical composition, transition metal, and microporous structures were prepared using in situ and secondary growth crystallization synthesis methods in/on stainless steel porous disks. All zeolite materials were been used in catalase adsorption to analyze the zeolite behavior andthe effect of chemical composition and structure on interaction with the enzyme. This study shows that the electrostatic type of interaction seems to be of the utmost importance in influencing immobilization, while the zeolite Brönsted acidity of the support is the subordinate parameter, which differentiates the adsorption performances of different zeolite structures (that distinct for chemical composition of the framework). Moreover, it permits to conclude that transition metal-containing membranes adsorb a higher percentage of the enzyme with respect to no-exchanged membranes and that, for all materials synthesized, the amount of catalase adsorbed onto the zeolite crystals and membranes increases with the temperature.
Micellar Effect On Dephosphorylation Of Bis-4-Chloro-3,5-Dimethylphenylphosph...IOSR Journals
The rate enhancement depends on the hydrophobicity of the nucleophile. The micellar catalyzed reaction between bis-4-chloro-3,5-dimethylphenylphosphate ester and hydroxide or hydroperoxide anions has been examined in buffered medium (pH 8-10). First order rate constant (Kψ) for the reaction of hydroxide ion with bis-4-CDMPP go through maxima with the increasing concentration of cetyltrimethylammoniumbromide (CTABr). Micelles of CTABr very effective catalyst to the reactions of phosphate diesters. Rate constants measured with OH2- ions are approximately twice and thrice than that of OH- ions in presence of CTABr.
Micellar Effect On Dephosphorylation Of Bis-4-Chloro-3,5-Dimethylphenylphosph...IOSR Journals
The rate enhancement depends on the hydrophobicity of the nucleophile. The micellar catalyzed reaction between bis-4-chloro-3,5-dimethylphenylphosphate ester and hydroxide or hydroperoxide anions has been examined in buffered medium (pH 8-10). First order rate constant (Kψ) for the reaction of hydroxide ion with bis-4-CDMPP go through maxima with the increasing concentration of cetyltrimethylammoniumbromide (CTABr). Micelles of CTABr very effective catalyst to the reactions of phosphate diesters. Rate constants measured with OH2- ions are approximately twice and thrice than that of OH- ions in presence of CTABr.
Fibrous Scaffold Produced By Rotary Jet Spinning TechniqueIJERA Editor
Poly(L-lactic acid) (PLLA)/ poly(ɛ-caprolactone) (PCL) mesh was produced by Rotary Jet Spinning (RJS)
process. RJS is a simple method which fabricates three-dimensional fibers by exploiting a high-speed rotating
nozzle o form a polymer jet which undergoes stretching before solidification without the need of high voltage.
Blend meshes were characterized by scanning electron microscopy (SEM), thermo gravimetric analysis (TGA),
differential scanning calorimeter (DSC) and infrared spectroscopy Fourier transform (FTIR). SEM imagens
provides information about the morphological structure, which confirmed the production of fibers using RJS.
Data obtained by thermal analyzes indicated the immiscible property of PLLA/PCL blend and also the total
solvent evaporation. As a preliminary in vitro assay it was investigated using Vero cells, was not found any sign
suggesting cell toxicity, indicating biocompatibility. Thus, this report suggests the use of PCL/PLLA mesh as
fiber scaffold substrate for tissue engineering
Biocompatibility of Poly (L-Lactic Acid) Synthesized In Polymerization Unit B...IJERA Editor
The absorbable polyacid is one of the most used and studied materials in tissue engineering. This work
synthesized a poly (L-lactic acid) (PLLA) through ring-opening polymerization and produced nanofibers by the
electrospinning process. The PLLA was analyzed by FTIR and the cytotoxicity was evaluated by the MTT assay
and Live/Dead®. The hemocompatibility was tested by platelet adhesion and hemolytic activity assay. The tests
were performed in contact with human mesenchymal cells at varying times. The high rates of cell viability and
proliferation shown by MTT and Live/Dead® tests demonstrate that this PLLA is a non-toxic material and the
hemocompatibility assay revealed that the biomaterial was also biocompatible. It was achieved as well the
successful production of electrospinning nanofibers, which can be converted for specific biomedical applications
in the future
In the early twentieth century, major representatives of the Jadid movement became active participants in the socio-political processes in the Turkestan region. Usmonkhoja Polatkhoja, a progressive from Bukhara, was one of the beams not only in the Emirate of Bukhara, but also in Turkestan. He first participated in the reforms and progressives, and later in the national liberation movements, and fought for the prosperity and independence of the country.This article provides information about Usmonkhoja's life and work in Jadidism, revolts, national liberation struggles, and emmigiration.
Flood is one of the natural disaster known to be part of the earth biophysical processes, which its occurrence can be devastating; due to mostly anthropogenic activities and climatological factors. The aim of the research is to identify and map the extent at which the impact of flood due to intense rainfall and rise in water in the study area using geospatial techniques and the specific objectives are to carry out terrain analysis of the study area and to generate flood indicator maps of the study area. The study analyzed rain fall data;, the drainage system and Shuttle Radar Topographic Mission (SRTM 30m) of the area. ArcGIS 10.8 was to modelled and to generate the contributing factors map of the study area. The drainage system was generated through on-screen digitization of topographic map of scale 1:50,000 of Ondo South-West. The mean annual rainfall of Lagos State was generated in the ArcGIS environment from the rainfall data through spatial analysis tool. The SRTM was used in terrain analysis of the study area. The results generated showed the lowest mean annual rain fall of the area 1,700mm and the highest mean annual rain fall was 2,440mm. Digital elevation model (DEM), slope, flow direction were generated from the SRTM. Drainage density of the area was generated using the drainage system. The slope map of the entire area which are classified into five slope classes of very high (14%-48.5%) to high (7.6%-13.9%) to moderately high (4.2%-7.6%) to low (1.5%-4.2%) and very low (0. % - 1.2%).
Work study is a catch-all phrase encompassing a variety of methodologies, including method research and work measurement, that are applied in a variety of contexts and lead to a systematic assessment of all elements that affect the efficiency and economy of the situation under evaluation that is meant to be improved. The main aim of this study is to examine and enhance the process token in manufacturing a Perfume of the famous, well-known, aromatic, and beautiful Taif Roses. Some changes in the process has been suggested using method study and time study method which lead to reduction in process time, labor cost and production cost.
Workers are the maximum precious method of an association. Their importance to institutions requires not most effective the want to draw the trendy bents but additionally the need to preserve them for a long term. This paper specializes in reviewing the findings of former research carried out with the aid of colourful experimenters with the quit to identify determinants factors of hand retention. This exploration almost looked at the subsequent broad factors improvement openings, reimbursement, work- lifestyles balance, operation/ management, work terrain, social aid, autonomy, training and improvement.
Watering plants during the correct time is very important due to scientific reasons. Both underwatering, as well as overwatering, can lead to the growth of unhealthy plants or in extreme cases, the death of the plant/tree. These issues which are the case with most self-gardeners and plant lovers can be solved using the smart irrigation technique. The main purpose of this innovation is to assist plant lovers to continue their passion to grow plants at home with ease. Smart irrigation system helps in monitoring the moisture level which majorly affects plant growth besides other factors such as sunlight, fertility of the soil, etc. The digital planting pot has been designed in a way that it effectively incorporates the idea of smart irrigation. Arduino Uno R3 has been used as the main chip in this project along with a few other components like a soil moisture sensor, relay, and water pump. This project requires coding to synchronize all the components, and function properly. A required test has been carried out to review the functioning of the mechanism. The project was tested by once using the soil with enough moisture in the pot and then the soil with the least moisture. Both times, it worked exactly how it was supposed to function. When the soil with the least moisture was tested, there was a clear indication of a low level of moisture and accordingly, the water pump got triggered to water the plant, and when the soil with enough moisture was tested, there was again the clear indication of the correct level of moisture and the water pump was inactive. All the readings which were displayed on the LCD were checked back and forth during the project. The outcomes were the same as expected. Hence, it shows that every component in this project is actively functioning and the whole project is effectively designed.
Because of its accessibility and flexibility, cloud technology is among the most notable innovations in today's world. Having many service platforms, such as GoogleApps by Google, Amazon, Apple, and so on, is well accepted by large enterprises. Distributed cloud computing is a concept for enabling every-time, convenient, on-demand network access to processing resources including servers, storage devices, networks, and services that may be mutually configured. The major security risks for cloud computing as identified by the Cloud security alliance (CSA) have been examined in this study. Also, methods for resolving issues with cloud computing technology's data security and privacy protection were systematically examined.
This study's goal is to present Solutions for Determining the importance level of criteria in creating cultural resources’ attractiveness from tourists’ evaluation. Data were collected from 558 international tourists who chose Vietnam as the destination for tourism.
The study points out that we need to resolve challenges such as: building a safe, friendly destination, etc., destinations need to review and re-evaluate the services of their products and tourist attractions to prepare for the largest number of visitors and stimulate the domestic tourism market is a good solution: To boost the domestic tourism market, it is necessary to increase domestic flights and train connections to major tourist destinations.
A new convenient and efficient route for the synthesis of two very important hydroxo-bridged stepped-cubane copper complexes viz: [Cu4(bpy)4Cl2(OH)4]Cl2.6H2O (1) and [Cu4(phen)4Cl2(OH)4]Cl2.6H2O (2) have been obtained. This synthetic route from the mononuclear CubpyCl2 complex is easier, more reproducible and afforded the complex in a much higher yield than the other two previously reported procedures which were equally serendipitously discovered. The purity and formation of the complexes were confirmed with elemental (C,H,N) analysis and the details of the UV-Vis, Fourier transform infrared, electrospray ionization mass spectra of both complexes and the single crystal X-ray crystallography of 1 are presented and discussed. X-ray crystallography confirms the absolute structure of the complexes. The complexes were formed via the connection of four copper atoms to four hydroxide bridging ligands and four bipyridyl ligands with two chloride ligands. There are two coordinate environments around two pairs of copper atoms (CuN2ClO2 and CuN2O3) and each copper atom is pentacoordinate with square pyramidal geometry.
Artocarpus heterophyllus Lam., which is commonly known as jackfruit is a tropical fruit, belonging to Moraceae family, native to Western Ghats of India and common in Asia, Africa, and some regions in South America. It is known to be the largest edible fruit in the world. The Jackfruit is an extremely versatile and sweet tasting fruit that possess high nutritional value. Jackfruit is rich in nutrients including carbohydrates, proteins, vitamins, minerals, and phytochemicals. The jackfruit has diverse medicinal uses especially antioxidant, anti-inflammatory, antimicrobial and antiviral properties, anticancer and antifungal activity, anthelminthic activity. Traditionally, this plant is used in the treatment of various diseases especially for treatment against inflammation, malarial fever, diarrhoea, diabetes and tapeworm infection. Jackfruit is a good natural source of phytochemicals such as phenolics, flavonoids and tannins, saponins. The health benefits of jackfruit have been attributed to its wide range of physicochemical applications. The use of jackfruit bulbs and its parts has also been reported since ancient times for their therapeutic qualities. The beneficial physiological effects may also have preventive application in a variety of pathologies.
Myogenic differentiation requires to be exactly explored for the effective treatment of fracture. The speed of healing is affected by skeletal muscle, linked to activation of specific myogenic transcription factors during the repair process. In previous study, we discovered that psoralen enhanced differentiation of osteoblast in primary mouse. In the current study, we show that psoralen stimulates myogenic differentiation through the secretion of factors to hone the quality of repair in fractured mice. 3-month old mice were treated with corn oil or psoralen followed by a tibial fracture surgery. Fractures were tested 7, 14, and 21 days respectively later by histology and images observation. Skeletal muscles including soleus muscle and posterior tibial muscle around the damaged bone were collected for quantitative real-time PCR, HE staining, as well as western blot. Daily treatment with psoralen at seven, fourteen days or twenty-one days improves protein or mRNA levels responsible for the whole myogenic differentiation process, makes the muscle fibers more tightly aligned, and promotes callus formation and development. This data shows that high levels of myogenic transcription factors in the process of fracture healing in mice foster the repair of damaged muscles, and indicates a pharmacological approach that targets myogenic differentiation to improve fracture repair. This also reflects the academic thought of "paying equal attention to both muscles and bones" in the prevention and treatment of fracture healing.
The current pandemic has generated the search for new reliable and economic alternatives for the detection of SARS-CoV-2, which produces the COVID-19 disease, one of the recommendations by the World Health Organization, is the detection of the virus by RT-qPCR methods from upper respiratory tract samples. The discomfort of the pharyngeal nasopharyngeal swab described by patients, the requirement of trained personnel, and the generation of aerosols, are factors that increase the risk of infections in this type of intake. It is known that the main means of transmission of SARS-CoV-2 is through aerosols or small droplets, which is why saliva is important as a relevant means of detecting COVID-19. In this study, a modified method based on SARS-CoV-2 RNA release from saliva is described, avoiding the isolation and purification of the genetic material and its quantification of viral copies; the results are compared with paired pharyngeal/nasopharyngeal swab samples (EF/EN). Results showed good agreement in saliva samples compared to EF/EN samples. On average, a sensitivity for virus detection of 80% was demonstrated in saliva samples competing with EF/EN samples. The use of saliva is a reliable alternative for the detection of SARS-CoV-2 by means of RT-PCR in the first days of infection, having important advantages over the conventional method. Saliva still needs to be studied completely to evaluate the detection capacity of the SARS-CoV-2 nucleic acid, however, the described process is viable, due to the decrease in materials and supplies, process times, the increment in the sampling and improvement of laboratory performance.
A recent study establishes that since 1970, there has been an ecological gap between human needs and the planet's resources, with annual resource demand exceeding the bio-productivity of the planet. Specifically, humanity utilises equivalent of 1.75 earths to produce the ecological resources used, with half of this attributable to food consumption. The present work therefore seeks to provide an empirically-based insight into the environmental sustainability of the EF of food consumption in Ijebu Ode. A descriptive cross-sectional approach was used, and primary data were collected from 400 systemically sampled households via structured questionnaires and analysed descriptively using Microsoft Excel and inferentially using mathematical models for calculating ecological footprints. Findings revealed that the household EF of food consumption in Ijebu Ode is 0.05gha per capita, with the footprint of cereal consumption (0.17gha; 37%) taking the major share, followed by meat with a footprint of 0.11gha (23.9%). As a result, it was concluded that Ijebu Ode has sustainable food consumption, which is necessary for its environmental sustainability. However, the sustenance of the former requires creating awareness of the need for sustainable consumption and prioritisation of integrated and population-wide policies and food intervention initiatives to encourage attitudinal change in favour of sustainable food consumption while fostering sustainable food production strategies amidst current environmental realities.
The symmetry occurs in most of the phenomena explained by physics, for example, a particle has positive or negative charges, and the electric dipoles that have the charge (+q) and (-q) which are at a certain distance (d), north or south magnetic poles and for a magnetic bar or magnetic compass with two poles: North (N) and South (S) poles, spins up or down of the electron at the atom and for the nucleons in the nucleus In this form, the particle should also have mass symmetry. For convenience and due to later explanations, I call this mass symmetry or mass duality as follows: mass and mass cloud. The mass cloud is located in the respective orbitals given by the Schrödinger equation. The orbitals represent the possible locations or places of the particle which are determined probabilistically by the respective Schröndiger equation.
Exercise training with varying intensity increases maximal oxygen intake (VO2max), a strong predictor of cardiovascular and all-cause mortality. Purpose: The aim of this study was to find out the influence of low intensity aerobic training on the vo2 max in 11 to 14 years school girls in Hyderabad district. Methodology: The research scholar has randomly selected thirty (N=30) high school girls were selected as subjects and their age ranged between 11 to 14 years. The subjects were divided into two equal groups, each group consist of 15 total 30. Group one acted as experimental group (EG) and group two acted as control group (CG). The dependent variable vo2 max was selected and it is measured by manual test. Statistical Tool: The statistical tool paired sample ‘t’ test was used for analysing of the data and the obtained ‘t’ ratio was tested for significance at 0.05 level of confidence. Results: The analysis of the data revealed that there was a significant improvement on vo2 max by the application of low intensity aerobic.
Hybrid rice has the potential to outperform existing inbred rice and was said to have the potential to produce 14-20 % more yield. In response, Malaysia Government has introduced its very own first Hybrid Rice Variety knew as Kadaria 1 developed by MARDI. This is in line with one of the strategies outlined in Dasar Agromakanan Negara (DAN) 2011-2020 as an approach to increasing rice productivity within Malaysia. The next step would be developing our hybrid seed rice production system. Therefore, an experiment to determine the planting ratio and planting distance between 0025A (A)-a hybrid with MR283 (R)-inbreed variety was carried out. Planting ratios studied in this study were 2:4, 2:6, 2:8, and 2:10 while planting distance was 14 x 30 cm, 16 x 30 cm, and 18 x 30 cm. Statistical analyses suggested that yield R, yield A, and panicle number A were significantly affected by planting ratios while yield A was significantly affected by an interaction between planting distance and planting ratios. Panicle number A performed significantly higher at planting ratios of 2:4 compared to 2:10. Yield R shows higher significant performance under ratio 2:6 compared to 2:4 and 2:8. Relatively, yield A performed the best under planting distance of 18 x 30 cm. Furthermore, under this particular planting distance, the planting ratio of 2:10 shows the highest significant figure while 2:8 exhibits statistical parity. Both yield R and yield A were significantly affected by planting ratios and have a significant positive association with each other. Therefore, the planting ratio of 2:10 should be the best since it contributed to significantly highest value for yield A while yield R under 2:10 shows statistical parity with 2:6 which was the highest significant value. In conclusion, the combination of 2:10 with a planting distance of 18 x 30 cm was the best since it shows best potential for both yields A and yield R
Cassava plays an important role in improving food security and reducing poverty in rural areas. Despite its importance, its production in Senegal remains low compared to other African countries. Nowadays, it is confronted with numerous constraints. It is in this context that a study was conducted on the cassava production system in the Thiès "cassava granary" region, with the objective of examining farmers' cultivation practices. It was conducted in eight communes located in the department of Tivaouane, some of which are located in the Niayes agro-ecological zone and others in the central-northern groundnut basin. Surveys were conducted among the largest cassava producers in these communes. Analysis of the results showed that cassava is only grown in the rainy season with the same cultivation practices that have been used for years. Of the five varieties listed by the President of the Senegalese Cassava Interprofession, only four are grown in the areas surveyed. The Terrasse (43%) and Kombo (36%) varieties are grown more by our respondents in the Niayes area. Soya (75%) and Wallet "Parydiey" (20% of our sample) dominate in the central-northern groundnut basin.
Cassava plays an important role in improving food security and reducing poverty in rural areas. Despite its importance, its production in Senegal remains low compared to other African countries. Nowadays, it is confronted with numerous constraints. It is in this context that a study was conducted on the cassava production system in the Thiès "cassava granary" region, with the objective of examining farmers' cultivation practices. It was conducted in eight communes located in the department of Tivaouane, some of which are located in the Niayes agro-ecological zone and others in the central-northern groundnut basin. Surveys were conducted among the largest cassava producers in these communes. Analysis of the results showed that cassava is only grown in the rainy season with the same cultivation practices that have been used for years. Of the five varieties listed by the President of the Senegalese Cassava Interprofession, only four are grown in the areas surveyed. The Terrasse (43%) and Kombo (36%) varieties are grown more by our respondents in the Niayes area. Soya (75%) and Wallet "Parydiey" (20% of our sample) dominate in the central-northern groundnut basin.
We are witnessing very demanding and stressful times in which we live, and an occupation that is particularly exposed to stress and different working conditions is the job of a nurse. Exposing themselves to everyday challenges and stressful situations, nurses reach a stage of great emotional and physical exhaustion, lethargy, dissatisfaction, and poorer work achievements, which we know as burnout. The aim of this paper was to determine whether there is and to what extent professional burnout is present in nurses and technicians working in nursing homes across Slovenia and Croatia. The paper is answering the questions of the extent of the burnout influenced by individual characteristics (age, education, years of service and work experience at the current workplace). The study involved a validated questionnaire “The Oldenburg Burnout Inventory (OLBI)” to measure professional burnout. Surveying of the nurses was conducted online at their home institutions. The results show that all respondents have a medium or high level of professional burnout, while no one has a low level or shows no signs of burnout. In terms of age, the group from 55-65 years of age had the highest relative level of burnout in the age group category. With regard to education, the highest burnout was measured in registered nurses.
Hepatitis B and C are one of the most commonly transmitted viral infections through needlestick injury apart from HIV. It is highly prevalent in India and many other developing countries. It accounts for high mortality rate globally amongst low socio-economic groups of individuals. Healthcare workers, especially dental professionals are at higher risk of infection due to high exposure to saliva, blood and sharps. Accidental occupational exposure to non-sterile conditions and its development to more critical and fatal conditions can be reduced through vaccination, prophylactic medications and practicing high safety measures.
This review article focuses on transmission of hepatitis through sharps injuries in medicine, especially dentistry, its prevention, management, post-exposure prophylaxis and the corresponding content.
Radicular cysts are the most common cystic lesions affecting the jaws. They are most commonly found at the apices of the involved teeth, however they may also be found on the lateral aspects of the roots in relation to lateral accessory root canals. Quite often a radicular cyst remains behind in the jaws after removal of the offending tooth and this is referred to as a residual cyst. Radicular cysts are the most common of all jaw cysts and comprise about 52% to 68% of all the cysts affecting the human jaws.
More from Associate Professor in VSB Coimbatore (20)
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
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26
In the present work, it is proposed the synthesis of two molybdenum complex based on ammonium heptamolybdate
(AHM) and BDC or BHET as organic linker, where BHET was previously obtained from PET depolymerization.
The hydrothermal process was employed as the synthesis method using water as a solvent to assure the
biocompatibility of the compounds, avoiding traces of organic solvents. The crystal, textural, chemical structure,
thermal stability and biocompatibility of BDC-Mo and BHET-Mo were systematically studied obtaining
molybdenum-based complexes that crystalize in the form of rectangular prism composed of rods arrays, where
fibroblast can live up to 48 h without affecting their metabolism, as it is shown in the fluorescence images with
calcein (Fig.1), and thus these novel materials could be potential candidates for biomedical applications in tissue
engineering and controlled drug delivery.
Fig.1. Synthesis of molybdenum complexes and fibroblast proliferation using Live/Dead assay
2. Methods
2.1. Synthesis of molybdenum complexes
The BDC-Mo and BHET-Mo complexes were synthesized mixing 5 mmol of ammonium heptamolybdate
((NH4)6Mo7O24.4H2O, AHM) and 17.5 mmol of terephthalic acid (HOOC-C6H4-COOH, BDC) or 17.5 mmol of
bis(2-hydroxyethyl) terephthalate (HO-C2H4-OOC-C6H4-COO-C2H4-OH, BHET), the latter was obtained
previously by the depolymerization of PET bottles, the glycolysis procedure was described somewhere else [9].
The metallic precursor and the organic ligand were dissolved and mixed in 50 mL of distillated water, the solution
was put into a Teflon-lined steel autoclave and heated up to 120ºC for 72 h. The hydrothermal reactor was opened
containing a blue-colored suspension that was filtrated under vacuum, obtaining a whitish precipitate which was
washed with distillated water, and it dried at 80ºC during 12 h.
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27
2.2.Physicochemical characterization
The XRD diffractograms of BDC-Mo and BHET-Mo complexes as well as their precursors were acquired in a 2θ
range from 5º to 40º with a Cu Kαx ray source (λ=1.540 Å) using a SAXS-Emc2, Anton Paar diffractometer. The
infrared spectra of BDC-Mo and BHET-Mo materials were acquired with a resolution of 16 cm-1
from 4000 to 600
cm-1
using Perkin Elmer Frontier equipment.
The X-ray photoelectron analysis (XPS) of BDC-Mo and BHET-Mo was done employing a JEOL photoelectron
spectrometer model JPS-9200 equipped with a monochromatic Mg X-ray radiation (300 W, 15 kV and 1253.4 eV).
The powders of materials were deposited on a conducting scotch tape and located in Al tapes on stainless steel
sample holders, which remained in a pre-analysis chamber until a pressure of 10−3
mbar was achieved before
entering the analysis chamber. Survey spectra were recorded from 0 to 1100 eV at constant pass energy of 100 eV,
onto 900 µm2
of analysis area; narrow spectra of C1s, O1s, N1s and Mo3d regions were recorded in the constant
pass energy mode at 20 eV, dwell 100 and 10 scans at least, Specsurf version 1.8.2 was used for acquisition. Origin
v.9.0 was used for data analysis, the charge correction was adjusted with carbon signal C1s 284 eV. A Shirley type
background was subtracted as baseline from the spectra and signal was deconvoluted using Gaussian curves to
determine binding energies of the different element core level. NIST database was used to identify the
corresponding element of the measured binding energies.
The textural properties such as surface area and mean pore diameter of BDC-Mo and BHET-Mo complexes were
analyzed by N2 adsorption using the BET method. The nitrogen physisorption experiments were carried out in a
Quantachrome Autosorb iQ instrument, with a previous outgassing of the samples at 200ºC during 12 h. The
thermal properties of materials were measured in a TGA-4000 Perkin Elmer thermoanalyzer, where 5 mg of sample
were heated since room temperature until 800ºC with a heating rate of 20ºC/min, and a N2 flow of 20 mL/min as
inert atmosphere. The SEM micrographs were acquired in a JEOL JSM-6510LV scanning electron microscope.
2.3. Analysis of in vitro biocompatibility
The viability of porcine fibroblasts growing on water suspensions of molybdate complexes was assessed.
Fibroblasts suspension in DMEM culture medium was added to every well (50,000 cells) that contain every
molybdenum complex in a water suspension and the control (phosphate buffer solution (PBS 1X)), these were
incubated for 24 and 48 h. The cell viability was determined by the active metabolism of these cells to transform
MTT salts into formazan with respect to control. The MTT (1% w/v) was added to wells with materials and
controls, and afterward, the cells were kept up under culture conditions for 3 h at 37 °C. At that point, the medium
was tapped, the blue formazan was diluted in isopropanol, and the absorbance of the solutions was estimated at 560
nm, using a Thermo Scientific Multiskan Sky spectrophotometer. The absorbance of generated formazan by cells
growing in the control (PBS 1X) is equal to 100% of viability, thus the cell viability ratio was calculated dividing
the obtained absorbance by complex water suspension between the control absorbance.
3. Results and Discussion
Fig.2a shows the diffractograms of BHET, (NH4)6Mo7O24.4H2O and BDC. The BDC sample exhibits the peaks
17.3°, 25.1°, and 27.8° at 2θ that correspond to the double hydrogen bonded molecular chains of BDC leading to
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28
the form of plates and needles [11]. While the XRD of BHET shows characteristic signals at 2θ=10.2°, 16.3°, 21.1°,
23.3°, and 27.4° typical of crystals in the form of needles [12]. The XRD pattern indicates intense signals at
2θ=11.9°, 12.2° and 12.60° which are in line with the ICDD PDF card 21-0571 related to molybdenum oxide
ammonia hydrate confirming the crystal structure of ammonium heptamolybdate.
Fig.2b depicts the XRD patterns of BDC-Mo and BHET-Mo, most of the characteristic peaks are associated to the
crystal structure of ammonium tetramolybdate ((NH4)2Mo4O13, ICDD PDF card 80-0756) with intense signals at
2θ= 12.3°, 13.7°, 14.7°, 25.6°, 26.9° y 30.4° related to the planes (0 2 1), (1 1 1), (0 2 2), (2 1 2), (0 4 3), and (1 0 6),
respectively [13]. The characteristic diffraction peaks of BDC with a smooth left shift of 0.1° appear in both
BDC-Mo and BHET-Mo indicating the coordination bonding between the ligand and molybdenum octahedrons.
Also, small peaks at 11.3°, 18.9°, 21.8°, and 23.5° indicate the presence of the biuret crystal phase (NH(CONH2)2)
[14]. In addition, the crystal data of both BDC-Mo and BHET-Mo is provided in Table 1, these data were obtained
with the software FullProof Suite ToolBar using DicVol04 tool by selecting the 20 most intense peaks, it was
obtained that Mo-BDC and Mo-BHET possess a monoclinic crystal system typical of molybdenum complexes [15,
16] with a space group P 2/m.
Fig. 2. XRD patterns of (a) precursors and (b) molybdenum complexes
Table 1. Crystal data and textural properties of molybdenum complexes
PROPERTY BDC-MO BHET-MO
Crystal system Monoclinic Monoclinic
Space group P 2/m P 2/m
a (Å) 19.2875 13.7498
b (Å) 15.3559 14.3278
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c (Å) 7.8349 10.6793
α (°) 90 90
β (°) 91.17 107.01
γ (°) 90 90
Volume (Å3
) 2320.02 2011.80
BET surface area (m2
/g) 1.592 2.486
BJH pore diameter (nm) 1.716 1.388
Pore volumen (cm3
/g) 0.003 0.008
The SEM images of BDC-Mo and BHET-Mo are shown in Fig.3 evidencing that molybdenum complexes present
rectangular prism particles which are typical of molybdenum oxides (MoO3) generating rods arrays [17], and other
complexes based on molybdenum and imidazole as ligand [18, 19]. The only difference between BDC-Mo and
BHET-Mo is that the rectangular prism particles are more flattened in BHET-Mo than in BDC-Mo, thus obtaining
more rods arrays in BHET-Mo, this correlates with the cell angles calculated (Table 1).These results also imply the
major surface area and volume pore for BHET-Mo in comparison with BDC-Mo, however BDC-Mo shows a
slightly greater pore diameter than BHET-Mo, this being typical for microporous materials (Table 1).
Fig.3. SEM micrographs of (a) BDC-Mo and (b) BHET-Mo
The ATR-FTIR spectra of the BDC and BHET precursors are shown in Fig.4a. Both precursors exhibit similar
bands at 3200-3000 cm-1
, 1570 cm-1
, and 1400 cm-1
that are related to the C-H bonds and the C-C stretching
vibrations of the benzene ring [14, 20]. Also, there are two bands at 1280 cm-1
and 1720 cm-1
associated to the
vibration of C=O of the acyl group [14, 20]. The BDC shows an additional broad band at 940 cm-1
that belongs to
the bending vibration of -OH bond from the carboxyl group [14]; while BHET depicts additional bands at 1450
cm-1
and 3430 cm-1
that correspond to methylene bonds (-CH2) and hydroxyl bonds (-OH), respectively [20].
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Fig.4b plots the FTIR spectra of BDC-Mo and BHET-Mo complexes which are very similar. Both spectra indicate
broad bands (2900-3300 cm-1
) related to the stretching region of N-H and O-H bonds [14]. The band at 1720 cm-1
from BDC and BHET suffers a small shift to lower wavenumber at 1680 cm-1
obtaining a broad band, indicating the
carbonyl stretching in molecules containing the NH-CO-NH group, this shift is attributed to the coordination
through the oxygen atom with a metal to form complexes [14], this implies that carboxylic groups of BDC and
BHET ligands are losing hydroxyl groups forming coordination bonds, which explains the loss of the characteristic
band of hydroxyl groups of BHET in BHET-Mo complex. Also, the bending vibrations of the C=O bonds are found
as small bands at 630 cm-1
and 675 cm-1
evidencing that the coordination bonding in BDC-Mo and BHET-Mo is
mainly formed between molybdenum ions and carbonyl groups of BDC and BHET ligands [14]. In addition, the
bands at 1570 cm-1
, 1400 cm-1
and 1280 cm-1
are attributed to the C-H bonds, C-C stretching vibrations of the
benzene ring, and the vibration of the C=O of the acyl group. The bands at 884 cm-1
and 739 cm-1
represent the
stretching vibration of the Mo=O terminal group, and to the flexural vibration of the Mo-O-Mo group, respectively
[13].
Fig.4. ATR-FTIR spectra of (a) precursors and (b) molybdenum complexes
Fig.5 depicts the high resolution XPS spectrum of O1s of molybdenum complexes with their corresponding
deconvolution peaks. Both materials show deconvolution bands with similar binding energies, but BHET-Mo
shows and additional deconvolution peak to the three peaks obtained for BDC-Mo.
The first band for both materials centered in a binding energy of 530.2-530.3 eV that according to the XPS NIST
data base correspond to oxygen bonded to molybdenum as molybdate groups (MoO4
-2
). The second peak centered
in a binding energy of 531.3-531.7 eV is attributed to oxygen in compounds based on urea bonding (-NHCONH)
like the one found in biuret molecule (HN(CONH2)2). The third peak centered in a binding energy of 532.3-532.9
7. Asian Journal of Applied Science and Technology (AJAST)
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ISSN: 2456-883X www.ajast.net
31
eV is related to the oxygen of carbonyl groups attached to the benzene ring (-O=C-C6H5). While the additional peak
presented in the BHET-Mo complex at ~533.4 eV indicates the oxygen nearing the carboxyl groups related to the
ester bonds of the BHET linker which is in line with the results found in FTIR spectra.
Fig.5. XPS narrow spectra of O1s (a) BDC-Mo and (b) BHET-Mo
The thermogravimetric analysis of BDC-Mo and BHET-Mo are plotted in Fig.6a. The TGA plot for both materials
depicts a sharp weight loss between 32% to 35% for BHET-Mo and BDC-Mo, respectively, in a range temperature
of 300°C-375°C that is associated to the loss of ammonium ions bonded to molybdate groups [21] and the
decomposition of BDC and BHET ligands. However, the trace thermal degradation of ligands continues up to
543°C and 610°C for BDC-Mo and BHET-Mo complexes [22] leading to molybdenum oxides. BHET-Mo shows
higher resistance to endothermic decomposition. indicating that the nucleophilic hydroxyl groups in BHET
improve the structural stability of this molybdenum-based complex.
The biocompatibility of molybdenum complexes was analyzed with a fibroblast viability assay at 24 h and 48 h
(Fig.6b). The results of fibroblast viability probe indicate that both BDC-Mo and BHET-Mo materials exhibit an
enhanced cell viability higher than the control (PBS 1X). It is expected that the end carboxyl groups of BDC
guarantee the biocompatibility of BDC-Mo [5]. However, BHET-Mo maintains the cell viability up to 48 h of
incubation, this possibly obeys to the biocompatibility of ester bonding in this material as the XPS indicates [23].
As the fibroblast metabolism has a crucial role in skin tissue regeneration to obtain the wound closure in a minor
lapse of time. This indicated that BDC-Mo and BHET-Mo metal-organic complexes possess suitable
characteristics to be applied in biomedical applications such as tissue engineering, controlled delivery of drugs and
antiproliferative agent against cancer cells [16].
8. Asian Journal of Applied Science and Technology (AJAST)
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32
Fig.6. (a) Thermogravimetric analysis and (b) fibroblast viability of molybdenum complexes
4. Conclusion
The physicochemical characterization results indicated that molybdenum-based complexes are molybdate units
bonded to BDC and BHET ligand by urea bonding obtaining rectangular prism particles made up of thin rods arrays
typical of MoO3 assemblies and molybdenum complexes, belonging to the monoclinic system with a P 2/m group
space. The ATR-FTIR indicated that molybdenum is covalently coordinated to BDC and BHET ligands by their
carbonyl groups due the shift of their typical vibration band to lower wavenumbers. Also, the absence of bands at
around 3400 cm-1
related to terminal hydroxyl groups in BHET-Mo material indicated the loss of hydroxyl groups
which was confirmed by XPS indicating an additional peak of O1s related to ester bonding.
The high fibroblast viability of BDC-Mo and BHET-Mo compared with the control was attributed to the end
carboxyl groups of BDC, end ethoxy groups of BHET and urea bonding, however the best biocompatibility of
BHET-Mo was associated to the additional ester bonding present in this material. The biocompatibility shown by
molybdenum complexes indicated that these materials could be applied in tissue engineering devoted to healing
skin, and controlled drug delivery.
Declarations
Source of Funding
This research was funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) (grant FORDECYTPRO
NACES/6660/2020).
Competing Interests Statement
The authors declare no competing financial, professional, and personal interests.
9. Asian Journal of Applied Science and Technology (AJAST)
Volume 6, Issue 3, Pages 25-34, July-September 2022
ISSN: 2456-883X www.ajast.net
33
Ethical Approval
Based on institutional guidelines.
Consent for publication
Authors declare that they consented for the publication of this research work.
Availability of data and material
Authors are willing to share the data and material according to relevant needs.
Authors’ Contributions
All authors equally contributed to data collection, research, and paper drafting.
References
[1] Hille, R. (2002). Molybdenum and tungsten in biology. Trends Biochem. Sci., 27(7): 360–367.
[2] Mendel, R.R. (2005). Molybdenum: biological activity and metabolism. Dalton Trans., 21: 3404–3409.
[3] Zu, Y., Yao, H., Wang, Y., Yan, L., Gu, Z., Chen, C., Gao, L & Yin, W. (2021). The age of bioinspired
molybdenum-involved nanozymes: synthesis, catalytic mechanisms, and biomedical applications. View, 2(3):
20200188.
[4] Mendel, R.R. (2009). Cell biology of molybdenum. Biofactors, 35(5): 429–434.
[5] Pandey, A., Dhas, N., Deshmukh, P., Caro, C., Patil, P., García-Martín, M.L., Padya, B., Nikam, A., Mehta, T.
& Mutalik, S. (2020). Heterogeneous surface architectured metal-organic frameworks for cancer therapy, imaging,
and biosensing: a state of the art review. Coord. Chem. Rev., 409: 213212.
[6] Bhardwaj, N., Pandey, S.K., Mehta, J., Bhardwaj, S.K., Kim, K.H. & Deep. A. (2018). Bioactive
nano-metal-organic frameworks as antimicrobial against Gram-positive and Gram-negative bacteria. Toxical. Res,
7(5): 931–941.
[7] Feng, M., Zhang, P., Zhou, H.C. & Sharma, V.K. (2018). Water-stable metal-organic frameworks for aqueous
remvoal of heavy metals and radionuclides: A review. Chemosphere, 209: 783–800.
[8] Stoski, A., Viante, M.F., Santos Nunes, C., Curti Muñiz, E., Felsner, M.A. & Policiano Almeida, C.A. (2016).
Water-stable metal-organic frameworks for aqueous remvoal of heavy metals and radionuclides: A review. Polym.
Int., 65(9): 1024–1030.
[9] Cabrera-Munguia, D.A., León-Campos, M.I., Claudio-Rizo, J.A., Solis-Casados, D.A., Flores-Guia, T.E. &
Cano-Salazar, L.F. (2021). Potential biomedical applications of a new MOF based on a derived PET: Synthesis and
characterization. Bull. Mat. Sci., 44(4): 245.
[10] Cabrera-Munguia, D.A., Claudio-Rizo, J.A., Aguayo-Morales, H. & Martínez-Mora, E.I. (2022). Gel-based
on collagen-polyacrylate-MOF as an adjuvant in skin wound healing: a case study. Asian Journal of Basic Science
& Research, 4(1): 60–69.
10. Asian Journal of Applied Science and Technology (AJAST)
Volume 6, Issue 3, Pages 25-34, July-September 2022
ISSN: 2456-883X www.ajast.net
34
[11] Saska, M. & Myerson, A.S. (1985). Polymorphism and aging in terephthalic acid. Crystal Res. & Technol.,
20(2): 201–208.
[12] Castaño, V.M., Alvarez-Castillo, A., Vazquez-Polo, G., Acosta, D. & González, V. (1998). High resolution
electron microscopy of bis-(2-hydroxyethyl) terephthalate crystalline polymers. Microsc. Res. Tech., 40: 41–48.
[13] Yang, L., Li, X., Qi, T., Liu, G., Peng, Z. & Zhou, Q (2020). Direct synthesis of pure ammonium molibdates
from ammonium tetramolybdate and ammonium bicarbonate. ACS Sustainable Chem. Eng., 8(49): 18237–18244.
[14] Wang, M-L., Zhong, G-Q. & Chen, L. (2016). Synthesis, optical characterization, and thermal decomposition
of complexes based on biuret ligand. Int. J. Opt., 16: 5471818.
[15] Hou, G-F., Wang, X-D., Yu, Y-H., Gao, J-S., Wen, B. & Yan, P-F. (2013). A new topology constructed from
an octamolybdate and metallomacrocycle coordination complex. CrystEngComm., 15: 249–251.
[16] Joshi, A., Gupta, R., Sharma, D. & Singh M. (2021). Mo(VI) based coordination polymer as antiproliferative
agent against cancer cells. Dalton Trans., 50: 1253–1260.
[17] Maheswari, N. & Muralidharan, G. (2017). Controlled synthesis of nanostructured molybdenum oxide
electrodes for high performance supercapacitor devices. Appl. Surf. Sci., 416: 461–469.
[18] Cao, X., Zheng, B., Shi, W., Yang, J.., Fan, Z., Luo, Z., Rui, X., Chen, B., Yan, Q. & Zhang, H. (2015).
Reduced graphene oxide-wrapped MoO3 composites prepared by using metal-organic frameworks as precursor for
all solid state flexible supercapacitors. Adv. Mater., 27(32): 4695–4701.
[19] Zhang, X., Li, D., Dong, C., Shi, J. & Xu, Y. (2019). The synergistic supercapacitive performance of
Mo-MOF/PANI and its electrochemical impedance spectroscopy investigation. Mater. Today Commun., 21:
100711.
[20] Zhou, X., Lu, X., Wang, Q., Zhu, M. & Li, Z. (2012). Effective catalysis of poly(ethylene terephthalate) (PET)
degradation by metallic acetate ionic liquids. Pure Appl. Chem., 84(3): 789–801.
[21] Chithambararaj, A, Bhagya Mathi, D., Rajeswari Yogamalar, N. & Chandra Bose, A. (2015). Structural
evolution and phase transition of [NH4]6Mo7O2.4H2O to 2D layered MoO3-x. Mater. Res. Express, 2: 055004.
[22] Begüm Elmas Kimyonok, A. & Mehmet Ulutürk (2016). Determination of the thermal decomposition
products of terephthalic acid by using curie-point pyrolyzer. J. Energ. Mater., 34(2): 113–122.
[23] Undin, J., Finne-Wistrand & Albertsson, AN. (2016). Adjustable degradation properties and biocompatibility
of amorphous and functional poly(ester-acrylate)-based materials. Biomacromolecules, 15: 2800–2807.