1. Biobrick An adjective, not a noun http://en.wikipedia.org/wiki/Biobrick

3. Biobrick part in a plasmid http://partsregistry.org/Help:An_Introduction_to_BioBricks

4. The plasmid (backbone) http://partsregistry.org/Help:Plasmids http://partsregistry.org/Help:Plasmid_backbones/Glossary E EcoRI X XbaI S SpeI P PstI

5. Construction plasmids http://partsregistry.org/Part:BBa_P1010

6. Prefix & Suffix Prefix: GAATTC GCGGCCGC T TCTAGA G CTTCCG CGCCGGCG A AGATCT C Suffix: T ACTAGT A GCGGCCG CTGCAG A TGATCA T CGCCGGC GACGTC EcoRI XbaI SpeI PstI NotI Prefix cut with XbaI: ...GCT T^CTAG A G ...CGA A GATC^T C Suffix cut with SpeI: T A^CTAG T AGC... A T GATC^A TCG... SpeI + XbaI ...T A^CTAG A G... ...A T GATC^T C... http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix http://partsregistry.org/Help:Plasmid_backbones/Glossary

7. Standard assembly http://partsregistry.org/Help:Standard_Assembly_%28zoom%29

8. RBS-CDS issues http://partsregistry.org/Assembly:RBS-CDS_issues XbaI + SpeI (RBS)T A^CTAG A G(CDS) (RBS)A T GATC^T C(CDS) Scars: Normal:(RBS) T ACTAG A G (CDS) Scar of 8 bases With T:(RBS) T A CTAG ATG (CDS-ATG) Scar of 6 bases

9. Parallel/Rolling assembly http://partsregistry.org/Assembly:Rolling_assembly