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Bacterial Transformation: The pGLO system
The bacterial cells are plated on LB media alone and LB media-containing ampicillin and/or
arabinose to determine success of transformation (i.e. transformation efficiency) and to
manipulate expression of the green fluorescent protein.
complete the table below for the genotype of the E. Coli HB101 cells (use +/-)
Will E. coli (minus plasmid) be able to grow on Ampicillin?
Which plate(s) are expected to show transformation?
a) LB only plate: - DNA
b) LB/AMP plate: - DNA
c LB/AMP plate: + DNA
d) LB/AMP/ARA plate: +DNA
before transformation after successful transformation
Bla gene
GFP gene

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