Antimicrobial secondary metabolites isolated from a symbiotic bacterial strain of the Philippine sponge Hyrtios erecta (revised)
1. ANTIMICROBIAL SECONDARY METABOLITES ISOLATED FROM A SYMBIOTIC
BACTERIAL STRAIN OF THE PHILIPPINE SPONGE HYRTIOS ERECTA
Viviene S. Santiago[a]*†, Carissa Shipman[b] †, Jake Ivan P. Baquiran[b], Mark Jeremiah B.
Cleofas[a][b] , Gisela P. Concepcion, Ph.D[b], Lilibeth Salvador-Reyes, Ph.D[b].
[a] Institute of Chemistry, University of the Philippines, Diliman, Quezon City, Philippines
[b] Marine Science Institute, University of the Philippines, Diliman, Quezon City, Philippines
†These authors contributed equally
Marine invertebrates and their associated microorganisms have been a source of
compounds with diverse chemistry and significant bioactivity. In this study, the bioactive
principle of an associated microorganism from the Philippine sponge Hyrtios erecta was purified
using a bioassay-guided approach. Large-scale cultures were subjected to HP-20 Diaion®
fractionation to yield the crude methanolic extract. The crude extract possessed antimicrobial
activity against Staphylococcus aureus. Further purification of the crude extract was done using a
series of liquid-liquid partitioning, C18 reversed-phase and Sephadex LH-20® size exclusion
column chromatography. The resulting antimicrobial fraction was further subjected to C18
reversed-phase high performance liquid chromatography (HPLC) to yield two pure compounds.
Antimicrobial activity assessment on the pure compounds showed MIC90 of 32 and 8 μg/ml
against S. aureus. Total structure elucidation using various spectroscopic techniques is currently
being done.
Figure 1. Graphical abstract.