This document describes a study that used miRNA expression profiling to identify potential biomarkers for lung cancer. Researchers used the miRNeasy Serum/Plasma Kit to isolate total RNA from normal and lung cancer serum samples. They then used the miScript PCR System and the Human Serum & Plasma 384HC miScript miRNA PCR Array to analyze miRNA expression levels. Several miRNAs were found to be significantly upregulated in lung cancer serum samples, including miRNAs that have previously been identified as blood-based markers for lung cancer.