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Nano filtration and Demineralization of
Whey Liquid to Prepare Whey Liquid
Concentrate
Name: Ali Ammad Ahmad
Roll no: 100022
MS Food Science and Technology
Department of Food Science
Government College University Faisalabad
1
Introduction:
• Whey is a liquid, formed as a byproduct during the process of cheese
production
• It is composed of 93% of water and 50% of total solids from milk
• Whey protein concentrate are largely used as attractive food
ingredients in a wide range of food applications
• Once the whey has reached 80% protein, it can be dried to form whey
protein concentrate (WPC) powder
• Whey concentrate consists of 80% protein and 20% fat and sugar
(carbohydrates, lactose)
(Croissant, Kang, Campbell, Bastian, & Drake, 2009)
2
Introduction:
• The demineralization influences the shelf life of the whey concentrates
• Nano filtration (NF) offers the opportunity to concentrate and partially
demineralize whey at the same time
• Demineralized whey powder delivers improved functionality such as
mouthfeel and water binding properties
• And further use in Infant formulas
(Marx, Sixt, Hofsommer, Wörthmann, & Kulozik, 2019)
3
Objective
• To characterize the whey concentrate formed by Nano filtration
• Demineralization of whey concentrate to enhance its applications
4
Experimental design
Whey liquid
Nano Filtration
Demineralization Heat treatment
Storage
Analysis pH, TDS
Density, Brix
Mineral Analysis
5
Procurement of Materials
• The liquid whey will be purchased
from Adam Cheese.
• All the chemicals and glassware
required for the research will be
utilized from the respective lab.
6
Research Methodologies
pH
TDS
Density
Brix
Sedimentation
Fat Analysis
Mineral analysis
Statistical Analysis
All the tests will be carried out according to the recommended standards.
7
Research Methodologies
pH
 pH meter will be used
TDS
 TDS meter will be used to find the total dissolved solids
Brix
 Brix meter or refractometer will be used to find the brix of the concentrated
whey
(AOAC, 2016)
8
Research Methodologies
Sedimentation
 Sedimentation analysis will be done by using centrifuge machine
Fat Analysis
 It will be performed by using Gerber machine
Mineral analysis
 Spectrophotometer will be used to analyze minerals in concentrated whey
(Bekele, Nosworthy, Tyler, & Henry, 2021; Olorode & Sobowale, 2021)
9
Research Methodologies
Statistical Analysis
After analysis data will be collected and analyzed by using experimental design
(Montgomery, 2017)
10
References
• Bekele, E. K., Nosworthy, M. G., Tyler, R. T., & Henry, C. J. (2021). Antioxidant
capacity and total phenolics content of direct‐expanded chickpea–sorghum snacks.
Journal of Food Processing and Preservation, 45(5), e15439.
• Croissant, A., Kang, E., Campbell, R., Bastian, E., & Drake, M. (2009). The effect of
bleaching agent on the flavor of liquid whey and whey protein concentrate. Journal
of dairy science, 92(12), 5917-5927.
• Marx, M., Sixt, A., Hofsommer, J., Wörthmann, M., & Kulozik, U. (2019).
Manufacturing of demineralized whey concentrates with extended shelf life: Impact
of the degree of demineralization on functional and microbial quality criteria. Food
and bioproducts processing, 114, 1-11.
11
References
• Montgomery, D. C. (2017). Design and analysis of experiments: John wiley
& sons.
• Olorode, O. O., & Sobowale, S. S. (2021). Evaluation of qualities of extruded
snacks from yellow cassava flour substituted with processed sesame seed’s
flour. World Journal of Advanced Research and Reviews, 10(1), 074-086.
• Paez, V., Barrett, W. B., Deng, X., Diaz-Amigo, C., Fiedler, K., Fuerer, C.,
Konings, E. J. (2016). AOAC SMPR® 2016.002. Journal of AOAC
International, 99(4), 1122-1124.
12

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Ahmad (2)

  • 1. Nano filtration and Demineralization of Whey Liquid to Prepare Whey Liquid Concentrate Name: Ali Ammad Ahmad Roll no: 100022 MS Food Science and Technology Department of Food Science Government College University Faisalabad 1
  • 2. Introduction: • Whey is a liquid, formed as a byproduct during the process of cheese production • It is composed of 93% of water and 50% of total solids from milk • Whey protein concentrate are largely used as attractive food ingredients in a wide range of food applications • Once the whey has reached 80% protein, it can be dried to form whey protein concentrate (WPC) powder • Whey concentrate consists of 80% protein and 20% fat and sugar (carbohydrates, lactose) (Croissant, Kang, Campbell, Bastian, & Drake, 2009) 2
  • 3. Introduction: • The demineralization influences the shelf life of the whey concentrates • Nano filtration (NF) offers the opportunity to concentrate and partially demineralize whey at the same time • Demineralized whey powder delivers improved functionality such as mouthfeel and water binding properties • And further use in Infant formulas (Marx, Sixt, Hofsommer, Wörthmann, & Kulozik, 2019) 3
  • 4. Objective • To characterize the whey concentrate formed by Nano filtration • Demineralization of whey concentrate to enhance its applications 4
  • 5. Experimental design Whey liquid Nano Filtration Demineralization Heat treatment Storage Analysis pH, TDS Density, Brix Mineral Analysis 5
  • 6. Procurement of Materials • The liquid whey will be purchased from Adam Cheese. • All the chemicals and glassware required for the research will be utilized from the respective lab. 6
  • 7. Research Methodologies pH TDS Density Brix Sedimentation Fat Analysis Mineral analysis Statistical Analysis All the tests will be carried out according to the recommended standards. 7
  • 8. Research Methodologies pH  pH meter will be used TDS  TDS meter will be used to find the total dissolved solids Brix  Brix meter or refractometer will be used to find the brix of the concentrated whey (AOAC, 2016) 8
  • 9. Research Methodologies Sedimentation  Sedimentation analysis will be done by using centrifuge machine Fat Analysis  It will be performed by using Gerber machine Mineral analysis  Spectrophotometer will be used to analyze minerals in concentrated whey (Bekele, Nosworthy, Tyler, & Henry, 2021; Olorode & Sobowale, 2021) 9
  • 10. Research Methodologies Statistical Analysis After analysis data will be collected and analyzed by using experimental design (Montgomery, 2017) 10
  • 11. References • Bekele, E. K., Nosworthy, M. G., Tyler, R. T., & Henry, C. J. (2021). Antioxidant capacity and total phenolics content of direct‐expanded chickpea–sorghum snacks. Journal of Food Processing and Preservation, 45(5), e15439. • Croissant, A., Kang, E., Campbell, R., Bastian, E., & Drake, M. (2009). The effect of bleaching agent on the flavor of liquid whey and whey protein concentrate. Journal of dairy science, 92(12), 5917-5927. • Marx, M., Sixt, A., Hofsommer, J., Wörthmann, M., & Kulozik, U. (2019). Manufacturing of demineralized whey concentrates with extended shelf life: Impact of the degree of demineralization on functional and microbial quality criteria. Food and bioproducts processing, 114, 1-11. 11
  • 12. References • Montgomery, D. C. (2017). Design and analysis of experiments: John wiley & sons. • Olorode, O. O., & Sobowale, S. S. (2021). Evaluation of qualities of extruded snacks from yellow cassava flour substituted with processed sesame seed’s flour. World Journal of Advanced Research and Reviews, 10(1), 074-086. • Paez, V., Barrett, W. B., Deng, X., Diaz-Amigo, C., Fiedler, K., Fuerer, C., Konings, E. J. (2016). AOAC SMPR® 2016.002. Journal of AOAC International, 99(4), 1122-1124. 12