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Advanced Cell Biology good and best ppt on the mrna transport (1).pptx
1. Transport Across Biological
Membranes
By: Barnabas Tefera, Gashaw Yimer & Rafatuel Sija
Addis Ababa University, Institute of
Biotechnology –
Department of Molecular Biology
Speaker Notes This slide focuses on the proteins involved in mRNA nuclear export. As the mRNA matures, it interacts with various proteins, forming a complex called the mRNP. These proteins have different functions, with the EJC playing a crucial role in multiple processes. Other proteins might be involved in translation or directing the mRNA to specific locations in the cytoplasm.
Speaker Notes This slide dives deeper into the export process itself. The Mex67:Mtr2 complex plays a key role, with Mex67 needing adaptor proteins to effectively bind to mRNPs. These adaptor proteins can be various, including components of the EJC. The exact recognition mechanism for a mature mRNP remains under investigation. The need for adaptor proteins and the unclear recognition mechanism highlight the complexity of the process.
This first slide introduces the concept of mRNA export, a crucial step in protein production. We showcase messenger ribonucleoprotein (mRNP) complexes, which carry the genetic message encoded in mRNA, along with various proteins. The nuclear pore complex (NPC) is highlighted as the gateway for mRNPs to move from the nucleus (genetic control center) to the cytoplasm (protein production factory). The slide introduces the Brownian ratchet mechanism as the driving force behind this export process.
Speaker Notes This slide delves deeper into the Brownian ratchet mechanism. We emphasize its role in ensuring the directionality of the mRNP movement, preventing them from going back into the nucleus. The analogy of a one-way street is used to illustrate this point. The Mex67:Mtr2 heterodimer is introduced as a key player that facilitates the mRNP's passage through the NPC by interacting with FG-nucleoporins lining the pore. We explain how passive diffusion, the natural tendency for particles to move from areas of high concentration (nucleus) to low concentration (cytoplasm), drives the movement of mRNPs. Finally, we reiterate the importance of complex disassembly in the cytoplasm, where the mRNA needs to be free to be used for protein production.