ENOVEO presentation for the brokerage session of ACQUEAU Making water smarter workshop - Paris 09/16

1. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
7 place Antonin Poncet
Lyon, France
www.enoveo.com
o.sibourg@enoveo.com
Olivier Sibourg & Jean-Michel Monier
Real-time and in situ monitoring of aquatic environments using
indigenous microbial community-based biosensors

2. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
Objective
Develop a cost-effective event detection system to monitor
natural or industrial aquatic environments in real time
TOXIC COMPOUNDSORGANIC LOAD
to act as a decision support tool
to inform targeted sampling
by conventional means (ie spot sampling)

3. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
electrical signal reflects
microbial activity
Microbial biosensor (NODE) principle
biofilm development

4. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
0
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Time (days)
Signalintensity(mV)
Time required for
electrode colonisation (3-10 days)
Stable signal
* Real-time detection
(eg, toxic compounds, organic load…)
Functionalization of the biosensors
Starter / Inoculum
(sample from the site)

5. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
time
Signal
Increase or decrease in organic load toxicity
electrical signal directly reflects microbial activity
Examples of NODE responses to environmental changes

6. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
Natural and industrial aquatic environments
• Groundwater
• Rivers, lakes, oceans
• Bioprocesses
• Wastewater treatment plants and urban wastewater systems
Applications
• Detection of changes in organic load
• BOD5 assessment
• Detection of toxic compounds
• Assessment of pollutant concentration
• Characterization of process performance
• Monitoring efficiency, duration and extend of treatment
charge
toxicity
process
NODE applications

7. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
Objective : Detect the presence of potentially toxic compounds in industrial effluents
(different processes) before they’re sent to the biological treatment tank.
Toxicity assays (laboratory conditions)
Time (hours)

8. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
Biosensor response as a function of BOD5
Wastewater treatment plants
BOD5 100 to 1000 mg/l
Natural Environments
BOD5 < 100 mg/l
BOD5 (mg O2/l)
NODESignal(mV)
root-mean-square error (RMSE) : 31 mg O2/l
root-mean-square error (RMSE) : 2.9 mg O2/l

9. ACQUEAU Brokerage Session | 16 September, 2015 | Paris , France Olivier SIBOURG | ENOVEO | www.enoveo.com
Conclusions
• Indigenous microbial populations can be used as bioindicators for real-
time monitoring of aquatic environments.
• Microbial biosensors offer new perspectives for on-line monitoring and
can act as decision support tools.
• Advantages : on-line applications, autonomy, sensitivity and robustness
• Limitations : does not allow to identify the origin and nature of the
perturbations.
Perspectives :
• Development of compound-specific biosensors
• Metrological station (set of several on-line sensors)