8. Examine the figure shown in Panels B and C. What normal cellular structure contains branched cytoskeleton formations that resemble these? a. sarcomere d. lamellopodia b. microvilli e. adherens junctions c. filopodia 9. Given the data shown in Panels B and C, what do you think is moving after a baculoviral particle gets into the cell cytoplasm? a. baculoviral particle to the left d. actin filament fragments to the right b. baculoviral particle to the right e. actin filament fragments to the left c. baculoviral particle to the top 10. You are tracking vesicle transport by labeling vesicles in a neuron and watching them live. When you treat cells with taxol or keep them in the cold, the vesicles no longer move, although they are still visible. What kind of cytoskeletal element is required for this vesicle movement? a. actin d. both actin and tubulin b. tubulin e. neurofilaments c. intermediate filaments 11. Which of the following is an accurate general description of how a processive motor protein complex works? a. One tail domain binds to the cytoskeletal filament; the light chain domain catalyzes ATP hydrolysis; the neck domain undergoes a major conformational change to create the power stroke, swinging the other tail close to the filament for binding; the second tail binds, and the first is released b. One head (or stalk) domain binds to the cytoskeletal filament; this head domain catalyzes ATP hydrolysis; the neck domain undergoes a major conformational change to create the power stroke, swinging the tail close to the filament for binding; the tail binds, and the head (or stalk) is released c. One head (or stalk) domain binds to the cytoskeletal filament; this head domain catalyzes ATP hydrolysis; the tail domain undergoes a major conformational change to create the power stroke, swinging the other head (or stalk) close to the filament for binding; both the head (or stalk) come off the filament before the motor is reset and the next head (or stalk) binds d. One head (or stalk) domain binds to the cytoskeletal filament; this head domain catalyzes ATP hydrolysis; the neck domain undergoes a major conformational change to create the power stroke, swinging the other head (or stalk) close to the filament for binding; the second head (or stalk) binds, and the first is releasedImaging of baculovirus actin comet tails. Mueller J, Planzehter J, Winkler C, Nanta A et al. (2014) Fifi PLS novocr Above are several images from a recent paper "Electron Tomography and Simulation of Baculovirus Actin Comet Tails Support a Tethered Filament Model of Pathogen Propulsion". Although Baculovirus is a virus, it hijacks actin and its regulatory proteins in host cells to move within the cytoplasm and out of the cell, like Listeria does. Because the virus is small, it is easier to image in very high resolution. In panel A, localization of the viral particle, actin, and associated proteins along the actin comet tail are quantified. (If it is hard to see the colors,.

1. 8. Examine the figure shown in Panels B and C. What normal cellular structure contains branched
cytoskeleton formations that resemble these? a. sarcomere d. lamellopodia b. microvilli e.
adherens junctions c. filopodia 9. Given the data shown in Panels B and C, what do you think is
moving after a baculoviral particle gets into the cell cytoplasm? a. baculoviral particle to the left d.
actin filament fragments to the right b. baculoviral particle to the right e. actin filament fragments to
the left c. baculoviral particle to the top 10. You are tracking vesicle transport by labeling vesicles
in a neuron and watching them live. When you treat cells with taxol or keep them in the cold, the
vesicles no longer move, although they are still visible. What kind of cytoskeletal element is
required for this vesicle movement? a. actin d. both actin and tubulin b. tubulin e. neurofilaments c.
intermediate filaments 11. Which of the following is an accurate general description of how a
processive motor protein complex works? a. One tail domain binds to the cytoskeletal filament; the
light chain domain catalyzes ATP hydrolysis; the neck domain undergoes a major conformational
change to create the power stroke, swinging the other tail close to the filament for binding; the
second tail binds, and the first is released b. One head (or stalk) domain binds to the cytoskeletal
filament; this head domain catalyzes ATP hydrolysis; the neck domain undergoes a major
conformational change to create the power stroke, swinging the tail close to the filament for
binding; the tail binds, and the head (or stalk) is released c. One head (or stalk) domain binds to
the cytoskeletal filament; this head domain catalyzes ATP hydrolysis; the tail domain undergoes a
major conformational change to create the power stroke, swinging the other head (or stalk) close
to the filament for binding; both the head (or stalk) come off the filament before the motor is reset
and the next head (or stalk) binds d. One head (or stalk) domain binds to the cytoskeletal filament;
this head domain catalyzes ATP hydrolysis; the neck domain undergoes a major conformational
change to create the power stroke, swinging the other head (or stalk) close to the filament for
binding; the second head (or stalk) binds, and the first is releasedImaging of baculovirus actin
comet tails. Mueller J, Planzehter J, Winkler C, Nanta A et al. (2014) Fifi PLS novocr Above are
several images from a recent paper "Electron Tomography and Simulation of Baculovirus Actin
Comet Tails Support a Tethered Filament Model of Pathogen Propulsion". Although Baculovirus is
a virus, it hijacks actin and its regulatory proteins in host cells to move within the cytoplasm and
out of the cell, like Listeria does. Because the virus is small, it is easier to image in very high
resolution. In panel A, localization of the viral particle, actin, and associated proteins along the
actin comet tail are quantified. (If it is hard to see the colors, the curves of the graphs from left to
right correspond to top to bottom descriptions in the key). Panel B shows a negatively stained
image of the actin comet tail viewed by electron microscopy in 3D. The virus particle is on the right
(BV). Panel C depicts actin filaments from Panel B, with large black circles indicating the plus ends
(small circles indicate branch points). Scale bar is 100nm. Use this information and what you
learned in class to answer the following 4 questions. 6. Panel A shows that nucleating proteins
(Arp), branching (VASP), capping (CPb) and filament severing (cofilin) proteins are present along
the comet tail. Given this initial finding, which hypothesis do you think the authors intended to test?
a. Comet tail dynamics depend on multiple host proteins, not just nucleation, and tails may have
more complicated structures than that of a single filament. b. Comet tail dynamics depend on
multiple host proteins, including those needed to regulate microtubule dynamics. c. Comet tail
dynamics depend on multiple host proteins, not just nucleation, which all function to promote rapid

2. extension of a single filament. d. Comet tails are made up of many proteins (Arp, WASP, BP) that
all polymerize together to create a larger structure than just a thin actin filament. 7. Actin
polymerizing proteins are recruited to the surface of the pathogens to create comet tails.
Considering the data shown in the figures, and the authors' additional report that baculoviral comet
tails average 3.8+/1m in length, approximately how far from the virus is the highest level of
cofilin?a. 100nm d. 3.8m b. 1.9m e. 7.6m c. 0.95m8. Examine the figure shown in Panels B and C.
What normal cellular structure contains branched cytoskeleton formations that resemble these? a.
sarcomere d. lamellopodia b. microvilli e. adherens junctions c. filopodia 9. Given the data shown
in Panels B and C, what do you think is moving after a baculoviral particle gets into the cell
cytoplasm? a. baculoviral particle to the left d. actin filament fragments to the right b. baculoviral
particle to the right e. actin filament fragments to the left c. baculoviral particle to the top 10. You
are tracking vesicle transport by labeling vesicles in a neuron and watching them live. When you
treat cells with taxol or keep them in the cold, the vesicles no longer move, although they are still
visible. What kind of cytoskeletal element is required for this vesicle movement? a. actin d. both
actin and tubulin b. tubulin e. neurofilaments c. intermediate filaments 11. Which of the following is
an accurate general description of how a processive motor protein complex works? a. One tail
domain binds to the cytoskeletal filament; the light chain domain catalyzes ATP hydrolysis; the
neck domain undergoes a major conformational change to create the power stroke, swinging the
other tail close to the filament for binding; the second tail binds, and the first is released b. One
head (or stalk) domain binds to the cytoskeletal filament; this head domain catalyzes ATP
hydrolysis; the neck domain undergoes a major conformational change to create the power stroke,
swinging the tail close to the filament for binding; the tail binds, and the head (or stalk) is released
c. One head (or stalk) domain binds to the cytoskeletal filament; this head domain catalyzes ATP
hydrolysis; the tail domain undergoes a major conformational change to create the power stroke,
swinging the other head (or stalk) close to the filament for binding; both the head (or stalk) come
off the filament before the motor is reset and the next head (or stalk) binds d. One head (or stalk)
domain binds to the cytoskeletal filament; this head domain catalyzes ATP hydrolysis; the neck
domain undergoes a major conformational change to create the power stroke, swinging the other
head (or stalk) close to the filament for binding; the second head (or stalk) binds, and the first is
released