The document summarizes methods used to collect ancient and modern fish bones from archaeological sites and middens in Penobscot Bay, Maine. Samples of fish bones were collected from three sites to analyze changes in the local diet over time. Specific fish species targeted included nearshore flounder and offshore cod and sculpin. Carved fish hooks found in the middens provided evidence that fish was an important part of the diet. Modern samples of the same fish species were also collected for comparison.