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DACTYLORHIZA HATAGIREA
(PANCHAUNLE)
Shailendra shah
Department of pharmaceutical science,
Nobel college
Pokhara University
Pokhara University
School of Health and Allied Sciences
OVERVIEW
 Nomenclature
 Biological source
 Habitat
 Cultivation & Collection
 Microscopic and Macroscopic characters
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PharmacognosySeminar1
Pokhara University
School of Health and Allied Sciences
NOMENCLATURE
Kingdom : Plantae
Division: Angiosperm
Class: Liliopsida
Order : Asparagales
Family : Orchidaceae
Genus : Dactylorhiza
Species : D. hatagirea
English name : Marsh orchid
Vernacular name : Panchaunle
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School of Health and Allied Sciences
BIOLOGICAL SOURCE
Root is used as the medicine.
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HABITAT
 It is the native of Himalaya. It is found throughout
from west to east at temperate to subalpine
biocliates with in 2800-4000m altitude. It grows well
in moist places, open areas, shrub land and open
meadows. Flowering: June-July
 Fruiting: August- September
 Harvesting: September-November
 Stem: Erect, about 1 to 3 feet, apex part of it bears
leaves
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CULTIVATION AND COLLECTION
 The materials for propagation are seeds and tuber
cuttings. Plants can be grown by splitting the sprouting
tubers.
 Collected seeds did not show germination at controlled
temperature and moisture (temp.10-15c; humidity
90+5%) conditions.
 It probably requires thawing under the snow for about 4-5
months and mycorrhizal association for germination.
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School of Health and Allied Sciences
 The tolerable thermal range for the species varies
from 15 to 35ºC. However, the plant growth has
been noted to be optimal under the range of 15-
25ºC in the high altitude regions of Himalayas.
Collection: Though the collection of roots of
D. hatagirea is banned, generally tubers are
collected from wild selectively during October
leaving young buds. They are collected when plants
are of 2 years.
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School of Health and Allied Sciences
MICROSCOPIC CHARACTER
 Chromosome number: 2n = 42
 T.S. of root: Transverse section of D. hatagirea root
shows more or less smooth and elliptical out line
with outermost epiblema and massive ground
tissue.
 Epiblema is the outermost layer, which is made up
of single layered compactly, arranged barrel shaped
cells without intracellular space. Epiblema is
followed by multilayered paraenchymatous ground
tissue and is covered by thick brown cuticle .
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School of Health and Allied Sciences
 There is no distinction of hypodermis, cortex,
endodermis, pericycle and pith.
 The paranchymatous cells contain many starches,
tannins, albumin, mucilage and large mucilage
canal cells are found scattered in the ground tissue,
many exarch and closed vascular bundles are
scattered in the ground tissue.
 Xylem parenchyma is distinct but phloem and
cambium are not distinct.
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MACROSCOPIC CHARACTERS
 Roots: Roots are tuberous, yellowish, white or
grayish in color, rounded , ovate or digitate and
divided in to three or five finger like lobes, having
somewhat wrinkled appearance and hard corny.
 Leaves: Leaves are 6-12 cm long, 2-4 cm wide,
oblanceolate, base sheathing
 Flowers: Dense cylindrical spike, narrowly
lanceolate, curved, cylindrical spur, sepals and
petals nearly equal, shallowly 3 lobed and with
purple spots.
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School of Health and Allied Sciences
CHEMICAL CONSTITIEUNTS
 From the root of Dactylorhiza hatagirea, which are
termed Panch Aunle in Nepal, five new compounds
called dactylorhin A, B, C, D, E and two new natural
compounds named dactylosis A and B have been
isolated with twelve known compound.
 Dactylosis A and B have been reported from plant
growing in Nepal. Their action is expectorant,
Aphrodisiac, and nervine tonic.
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School of Health and Allied Sciences
 Leaves contain loroglossin, albumin, hydroquinone,
lesoglossin, militarrin, pyranoside, pyrocatechol and
volatile oil. Tuberous roots contain mucilage, starch,
dactylose A and B, dactylorhin A - E, 4-(β-D-
glucopyranosyl) benzyl alcohol, (2R)-2-hydroxy-2(2-
methylpropyl) butanedic acid, 4-hydroxybenzyl
methyl ether, 4-hydroxybenzaldehyde,
pyrocatechol, hydroquinone, 4hydroxybenzyl
alcohol and β-sitosterol-3-O-β-D-glucopyranoside.
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School of Health and Allied Sciences
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School of Health and Allied Sciences
fig: Dactylorhin A
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PHARMACOLOGICAL ACTION
 Ethnobotanical uses:
 D. hatagirea is famous tonic and aphrodisiac crude
drug in Tibetan, Ayurveda, Unani traditional
medicines.
 The decoction of tuber mixed with milk, sugar and
spice is given colic pain, stomach trouble. Root
tubers are boiled with milk and taken as a
rejuvenating tonic and aphrodisiac.
 A paste obtained from the tuber is applied
externally to cure wounds and cuts.
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School of Health and Allied Sciences
 It is also effective in diarrhea, dysentry, fever, debility,
paralytic, and afflictions.
 Tubers are eaten as a source of vitamins. It is used as
astringent, expectorant, nervine tonic, demulcent, and
urinary trouble.
 Root paste is often used in promoting growth and
blackness of hair.
 West Nepal: Root power is spread on wounds to
control bleeding and decoction of roots is utilized in
treating stomach troubles at Jumla district.
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School of Health and Allied Sciences
 Central Nepal: Pulverized and mixed with water,
roots are made into a slave and applied as a
disinfectant on wounds, particularly in Manag
district. Paste of rhizome is applied on cuts and
wounds by the Sherpa‟s of Helambu for speedy
healing.
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 Pharmacological action:
 Aphrodisiac activity: In traditional medicine, D.
hatagirea is consider as an important aphrodisiac
plant and employed to enhance performance as
well as to increase vigor and vitality. It promotes
the testerone production and its availability to
gonads which is associated with a moderate but
significant increase in sexual desire as well. It
provides purification to G.i.tract. ( Thakur and Dixit
2010)
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School of Health and Allied Sciences
 Antibacterial activity:
It has antibacterial activity against the bacteria
namely Staphylococcus aereus, E.coli, Shigella
dysrnteriae, Pseudomonas aureginosa and Bacillus
subtills. ( Ranpal 2009)
 On GIT
It provides purification to gastrointestinal tract. ( Kala
et al 2004)
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 On CNS
It provides strength to the nervous system due to
sweet taste.
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PHARMACOPOEIAL STANDARD
Standards Amount
Foreign organic matter Not more than 2%
Total ash Not more than 8%
Acid insoluble ash Not more than 2%
Loss on drying Not more than 12 %
Rf value 0.45
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COMMERCIAL VALUE
 Part used: root
 Current market trend: Rs700|kg
 D. hatagirea has been identified as the endangered
species listed by CITIES and vulnerable species
listed by CAMP and is banned from harvesting.
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FORMULATION
 Passion capsule cause Female Sexual Arousal,
sustained stamina and vitality.
 It increases confidence and sexual desire.
 It is likely that the extracts help in improving the
testosterone availability to gonads.
 Dosage: 1 to 2 capsule daily in a day before going
bed with milk or as directed by the physician.
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Source:
https://searchprivacy.co/?q=e4l3ZV2dIm&src=g&a=3001
07&chnm=sv9-107
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REFERENCES
 http://www.academicjournals.org/article/article1380
700617_Pant%20%20and%20Rinchen.pdf (
Accessed on June 5 2018)
 http://vikaspedia.in/agriculture/crop-
production/package-of-practices/medicinal-and-
aromatic-plants/dactylorhiza-hatagirea (Accessed
on June 7 2018)
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 Pant S., Rinchen T. (2012) Dactylorhiza hatagirea:
A high value medicinal orchid Journal of Medicinal
Plant Research vol 6 (19) page 3522- 3524
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3/8/2020PharmacognosySeminar1
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Dactylorhiza hatagirea (Panchaunle) Overview

  • 1. DACTYLORHIZA HATAGIREA (PANCHAUNLE) Shailendra shah Department of pharmaceutical science, Nobel college Pokhara University
  • 2. Pokhara University School of Health and Allied Sciences OVERVIEW  Nomenclature  Biological source  Habitat  Cultivation & Collection  Microscopic and Macroscopic characters 3/8/2020 2 PharmacognosySeminar1
  • 3. Pokhara University School of Health and Allied Sciences NOMENCLATURE Kingdom : Plantae Division: Angiosperm Class: Liliopsida Order : Asparagales Family : Orchidaceae Genus : Dactylorhiza Species : D. hatagirea English name : Marsh orchid Vernacular name : Panchaunle 3/8/2020 3 PharmacognosySeminar1
  • 4. Pokhara University School of Health and Allied Sciences BIOLOGICAL SOURCE Root is used as the medicine. 3/8/2020 4 PharmacognosySeminar1
  • 5. Pokhara University School of Health and Allied Sciences HABITAT  It is the native of Himalaya. It is found throughout from west to east at temperate to subalpine biocliates with in 2800-4000m altitude. It grows well in moist places, open areas, shrub land and open meadows. Flowering: June-July  Fruiting: August- September  Harvesting: September-November  Stem: Erect, about 1 to 3 feet, apex part of it bears leaves 3/8/2020 5 PharmacognosySeminar1
  • 6. Pokhara University School of Health and Allied Sciences CULTIVATION AND COLLECTION  The materials for propagation are seeds and tuber cuttings. Plants can be grown by splitting the sprouting tubers.  Collected seeds did not show germination at controlled temperature and moisture (temp.10-15c; humidity 90+5%) conditions.  It probably requires thawing under the snow for about 4-5 months and mycorrhizal association for germination. 3/8/2020 6 PharmacognosySeminar1
  • 7. Pokhara University School of Health and Allied Sciences  The tolerable thermal range for the species varies from 15 to 35ºC. However, the plant growth has been noted to be optimal under the range of 15- 25ºC in the high altitude regions of Himalayas. Collection: Though the collection of roots of D. hatagirea is banned, generally tubers are collected from wild selectively during October leaving young buds. They are collected when plants are of 2 years. 3/8/2020 7 PharmacognosySeminar1
  • 8. Pokhara University School of Health and Allied Sciences MICROSCOPIC CHARACTER  Chromosome number: 2n = 42  T.S. of root: Transverse section of D. hatagirea root shows more or less smooth and elliptical out line with outermost epiblema and massive ground tissue.  Epiblema is the outermost layer, which is made up of single layered compactly, arranged barrel shaped cells without intracellular space. Epiblema is followed by multilayered paraenchymatous ground tissue and is covered by thick brown cuticle . 3/8/2020 8 PharmacognosySeminar1
  • 9. Pokhara University School of Health and Allied Sciences  There is no distinction of hypodermis, cortex, endodermis, pericycle and pith.  The paranchymatous cells contain many starches, tannins, albumin, mucilage and large mucilage canal cells are found scattered in the ground tissue, many exarch and closed vascular bundles are scattered in the ground tissue.  Xylem parenchyma is distinct but phloem and cambium are not distinct. 3/8/2020 9 PharmacognosySeminar1
  • 10. Pokhara University School of Health and Allied Sciences MACROSCOPIC CHARACTERS  Roots: Roots are tuberous, yellowish, white or grayish in color, rounded , ovate or digitate and divided in to three or five finger like lobes, having somewhat wrinkled appearance and hard corny.  Leaves: Leaves are 6-12 cm long, 2-4 cm wide, oblanceolate, base sheathing  Flowers: Dense cylindrical spike, narrowly lanceolate, curved, cylindrical spur, sepals and petals nearly equal, shallowly 3 lobed and with purple spots. 3/8/2020 10 PharmacognosySeminar1
  • 11. Pokhara University School of Health and Allied Sciences CHEMICAL CONSTITIEUNTS  From the root of Dactylorhiza hatagirea, which are termed Panch Aunle in Nepal, five new compounds called dactylorhin A, B, C, D, E and two new natural compounds named dactylosis A and B have been isolated with twelve known compound.  Dactylosis A and B have been reported from plant growing in Nepal. Their action is expectorant, Aphrodisiac, and nervine tonic. 3/8/2020 11 PharmacognosySeminar1
  • 12. Pokhara University School of Health and Allied Sciences  Leaves contain loroglossin, albumin, hydroquinone, lesoglossin, militarrin, pyranoside, pyrocatechol and volatile oil. Tuberous roots contain mucilage, starch, dactylose A and B, dactylorhin A - E, 4-(β-D- glucopyranosyl) benzyl alcohol, (2R)-2-hydroxy-2(2- methylpropyl) butanedic acid, 4-hydroxybenzyl methyl ether, 4-hydroxybenzaldehyde, pyrocatechol, hydroquinone, 4hydroxybenzyl alcohol and β-sitosterol-3-O-β-D-glucopyranoside. 3/8/2020 12 PharmacognosySeminar1
  • 13. Pokhara University School of Health and Allied Sciences 3/8/2020 13 PharmacognosySeminar1
  • 14. Pokhara University School of Health and Allied Sciences fig: Dactylorhin A 3/8/2020 14 PharmacognosySeminar1
  • 15. Pokhara University School of Health and Allied Sciences PHARMACOLOGICAL ACTION  Ethnobotanical uses:  D. hatagirea is famous tonic and aphrodisiac crude drug in Tibetan, Ayurveda, Unani traditional medicines.  The decoction of tuber mixed with milk, sugar and spice is given colic pain, stomach trouble. Root tubers are boiled with milk and taken as a rejuvenating tonic and aphrodisiac.  A paste obtained from the tuber is applied externally to cure wounds and cuts. 3/8/2020 15 PharmacognosySeminar1
  • 16. Pokhara University School of Health and Allied Sciences  It is also effective in diarrhea, dysentry, fever, debility, paralytic, and afflictions.  Tubers are eaten as a source of vitamins. It is used as astringent, expectorant, nervine tonic, demulcent, and urinary trouble.  Root paste is often used in promoting growth and blackness of hair.  West Nepal: Root power is spread on wounds to control bleeding and decoction of roots is utilized in treating stomach troubles at Jumla district. 3/8/2020 16 PharmacognosySeminar1
  • 17. Pokhara University School of Health and Allied Sciences  Central Nepal: Pulverized and mixed with water, roots are made into a slave and applied as a disinfectant on wounds, particularly in Manag district. Paste of rhizome is applied on cuts and wounds by the Sherpa‟s of Helambu for speedy healing. 3/8/2020 17 PharmacognosySeminar1
  • 18. Pokhara University School of Health and Allied Sciences  Pharmacological action:  Aphrodisiac activity: In traditional medicine, D. hatagirea is consider as an important aphrodisiac plant and employed to enhance performance as well as to increase vigor and vitality. It promotes the testerone production and its availability to gonads which is associated with a moderate but significant increase in sexual desire as well. It provides purification to G.i.tract. ( Thakur and Dixit 2010) 3/8/2020 18 PharmacognosySeminar1
  • 19. Pokhara University School of Health and Allied Sciences  Antibacterial activity: It has antibacterial activity against the bacteria namely Staphylococcus aereus, E.coli, Shigella dysrnteriae, Pseudomonas aureginosa and Bacillus subtills. ( Ranpal 2009)  On GIT It provides purification to gastrointestinal tract. ( Kala et al 2004) 3/8/2020 19 PharmacognosySeminar1
  • 20. Pokhara University School of Health and Allied Sciences  On CNS It provides strength to the nervous system due to sweet taste. 3/8/2020 20 PharmacognosySeminar1
  • 21. Pokhara University School of Health and Allied Sciences PHARMACOPOEIAL STANDARD Standards Amount Foreign organic matter Not more than 2% Total ash Not more than 8% Acid insoluble ash Not more than 2% Loss on drying Not more than 12 % Rf value 0.45 3/8/2020 21 PharmacognosySeminar1
  • 22. Pokhara University School of Health and Allied Sciences COMMERCIAL VALUE  Part used: root  Current market trend: Rs700|kg  D. hatagirea has been identified as the endangered species listed by CITIES and vulnerable species listed by CAMP and is banned from harvesting. 3/8/2020 22 PharmacognosySeminar1
  • 23. Pokhara University School of Health and Allied Sciences FORMULATION  Passion capsule cause Female Sexual Arousal, sustained stamina and vitality.  It increases confidence and sexual desire.  It is likely that the extracts help in improving the testosterone availability to gonads.  Dosage: 1 to 2 capsule daily in a day before going bed with milk or as directed by the physician. 3/8/2020 23 PharmacognosySeminar1
  • 24. Pokhara University School of Health and Allied Sciences Source: https://searchprivacy.co/?q=e4l3ZV2dIm&src=g&a=3001 07&chnm=sv9-107 3/8/2020 24 PharmacognosySeminar1
  • 25. Pokhara University School of Health and Allied Sciences REFERENCES  http://www.academicjournals.org/article/article1380 700617_Pant%20%20and%20Rinchen.pdf ( Accessed on June 5 2018)  http://vikaspedia.in/agriculture/crop- production/package-of-practices/medicinal-and- aromatic-plants/dactylorhiza-hatagirea (Accessed on June 7 2018) 3/8/2020 25 PharmacognosySeminar1
  • 26. Pokhara University School of Health and Allied Sciences  Pant S., Rinchen T. (2012) Dactylorhiza hatagirea: A high value medicinal orchid Journal of Medicinal Plant Research vol 6 (19) page 3522- 3524 3/8/2020 26 PharmacognosySeminar1
  • 27. Pokhara University School of Health and Allied Sciences 3/8/2020PharmacognosySeminar1 27