This document provides an overview of Dactylorhiza hatagirea (Panchaunle), including its nomenclature, biological source, habitat, cultivation, microscopic and macroscopic characteristics, chemical constituents, pharmacological actions, and commercial value. The root of D. hatagirea is used as medicine and contains compounds such as dactylosis A and B. It grows in the Himalayas between 2800-4000m and flowers from June to July. Traditionally, it is used as an aphrodisiac, expectorant, and nervine tonic.
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OVERVIEW
Nomenclature
Biological source
Habitat
Cultivation & Collection
Microscopic and Macroscopic characters
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NOMENCLATURE
Kingdom : Plantae
Division: Angiosperm
Class: Liliopsida
Order : Asparagales
Family : Orchidaceae
Genus : Dactylorhiza
Species : D. hatagirea
English name : Marsh orchid
Vernacular name : Panchaunle
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BIOLOGICAL SOURCE
Root is used as the medicine.
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HABITAT
It is the native of Himalaya. It is found throughout
from west to east at temperate to subalpine
biocliates with in 2800-4000m altitude. It grows well
in moist places, open areas, shrub land and open
meadows. Flowering: June-July
Fruiting: August- September
Harvesting: September-November
Stem: Erect, about 1 to 3 feet, apex part of it bears
leaves
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CULTIVATION AND COLLECTION
The materials for propagation are seeds and tuber
cuttings. Plants can be grown by splitting the sprouting
tubers.
Collected seeds did not show germination at controlled
temperature and moisture (temp.10-15c; humidity
90+5%) conditions.
It probably requires thawing under the snow for about 4-5
months and mycorrhizal association for germination.
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The tolerable thermal range for the species varies
from 15 to 35ºC. However, the plant growth has
been noted to be optimal under the range of 15-
25ºC in the high altitude regions of Himalayas.
Collection: Though the collection of roots of
D. hatagirea is banned, generally tubers are
collected from wild selectively during October
leaving young buds. They are collected when plants
are of 2 years.
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MICROSCOPIC CHARACTER
Chromosome number: 2n = 42
T.S. of root: Transverse section of D. hatagirea root
shows more or less smooth and elliptical out line
with outermost epiblema and massive ground
tissue.
Epiblema is the outermost layer, which is made up
of single layered compactly, arranged barrel shaped
cells without intracellular space. Epiblema is
followed by multilayered paraenchymatous ground
tissue and is covered by thick brown cuticle .
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There is no distinction of hypodermis, cortex,
endodermis, pericycle and pith.
The paranchymatous cells contain many starches,
tannins, albumin, mucilage and large mucilage
canal cells are found scattered in the ground tissue,
many exarch and closed vascular bundles are
scattered in the ground tissue.
Xylem parenchyma is distinct but phloem and
cambium are not distinct.
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MACROSCOPIC CHARACTERS
Roots: Roots are tuberous, yellowish, white or
grayish in color, rounded , ovate or digitate and
divided in to three or five finger like lobes, having
somewhat wrinkled appearance and hard corny.
Leaves: Leaves are 6-12 cm long, 2-4 cm wide,
oblanceolate, base sheathing
Flowers: Dense cylindrical spike, narrowly
lanceolate, curved, cylindrical spur, sepals and
petals nearly equal, shallowly 3 lobed and with
purple spots.
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CHEMICAL CONSTITIEUNTS
From the root of Dactylorhiza hatagirea, which are
termed Panch Aunle in Nepal, five new compounds
called dactylorhin A, B, C, D, E and two new natural
compounds named dactylosis A and B have been
isolated with twelve known compound.
Dactylosis A and B have been reported from plant
growing in Nepal. Their action is expectorant,
Aphrodisiac, and nervine tonic.
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Leaves contain loroglossin, albumin, hydroquinone,
lesoglossin, militarrin, pyranoside, pyrocatechol and
volatile oil. Tuberous roots contain mucilage, starch,
dactylose A and B, dactylorhin A - E, 4-(β-D-
glucopyranosyl) benzyl alcohol, (2R)-2-hydroxy-2(2-
methylpropyl) butanedic acid, 4-hydroxybenzyl
methyl ether, 4-hydroxybenzaldehyde,
pyrocatechol, hydroquinone, 4hydroxybenzyl
alcohol and β-sitosterol-3-O-β-D-glucopyranoside.
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PHARMACOLOGICAL ACTION
Ethnobotanical uses:
D. hatagirea is famous tonic and aphrodisiac crude
drug in Tibetan, Ayurveda, Unani traditional
medicines.
The decoction of tuber mixed with milk, sugar and
spice is given colic pain, stomach trouble. Root
tubers are boiled with milk and taken as a
rejuvenating tonic and aphrodisiac.
A paste obtained from the tuber is applied
externally to cure wounds and cuts.
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It is also effective in diarrhea, dysentry, fever, debility,
paralytic, and afflictions.
Tubers are eaten as a source of vitamins. It is used as
astringent, expectorant, nervine tonic, demulcent, and
urinary trouble.
Root paste is often used in promoting growth and
blackness of hair.
West Nepal: Root power is spread on wounds to
control bleeding and decoction of roots is utilized in
treating stomach troubles at Jumla district.
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Central Nepal: Pulverized and mixed with water,
roots are made into a slave and applied as a
disinfectant on wounds, particularly in Manag
district. Paste of rhizome is applied on cuts and
wounds by the Sherpa‟s of Helambu for speedy
healing.
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Pharmacological action:
Aphrodisiac activity: In traditional medicine, D.
hatagirea is consider as an important aphrodisiac
plant and employed to enhance performance as
well as to increase vigor and vitality. It promotes
the testerone production and its availability to
gonads which is associated with a moderate but
significant increase in sexual desire as well. It
provides purification to G.i.tract. ( Thakur and Dixit
2010)
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Antibacterial activity:
It has antibacterial activity against the bacteria
namely Staphylococcus aereus, E.coli, Shigella
dysrnteriae, Pseudomonas aureginosa and Bacillus
subtills. ( Ranpal 2009)
On GIT
It provides purification to gastrointestinal tract. ( Kala
et al 2004)
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On CNS
It provides strength to the nervous system due to
sweet taste.
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PHARMACOPOEIAL STANDARD
Standards Amount
Foreign organic matter Not more than 2%
Total ash Not more than 8%
Acid insoluble ash Not more than 2%
Loss on drying Not more than 12 %
Rf value 0.45
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COMMERCIAL VALUE
Part used: root
Current market trend: Rs700|kg
D. hatagirea has been identified as the endangered
species listed by CITIES and vulnerable species
listed by CAMP and is banned from harvesting.
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FORMULATION
Passion capsule cause Female Sexual Arousal,
sustained stamina and vitality.
It increases confidence and sexual desire.
It is likely that the extracts help in improving the
testosterone availability to gonads.
Dosage: 1 to 2 capsule daily in a day before going
bed with milk or as directed by the physician.
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Source:
https://searchprivacy.co/?q=e4l3ZV2dIm&src=g&a=3001
07&chnm=sv9-107
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REFERENCES
http://www.academicjournals.org/article/article1380
700617_Pant%20%20and%20Rinchen.pdf (
Accessed on June 5 2018)
http://vikaspedia.in/agriculture/crop-
production/package-of-practices/medicinal-and-
aromatic-plants/dactylorhiza-hatagirea (Accessed
on June 7 2018)
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Pant S., Rinchen T. (2012) Dactylorhiza hatagirea:
A high value medicinal orchid Journal of Medicinal
Plant Research vol 6 (19) page 3522- 3524
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