- Thyroid hormones (T3 and T4) are synthesized from the amino acid tyrosine through a process involving iodination and coupling of tyrosine residues on the thyroglobulin protein within thyroid follicles. - T4 is converted to the more active T3 hormone in tissues by deiodinase enzymes. Thyroid hormones are secreted into the bloodstream and transported bound to carrier proteins while a small fraction of free T3 and T4 is biologically active. - Thyroid stimulating hormone (TSH) from the pituitary gland controls thyroid hormone production and secretion through a negative feedback loop with the hypothalamus and thyroid. Laboratory tests of thyroid function include TSH, free T