Stock solution calculation

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Stock solution Preparation

STOCK SOLUTION
CALCULATION
Dr. S. SIVASANKARA NARAYANI

FORMULA
• C1V1 = C2V2
C1 = Concentration of the stock solution
C2 = Concentration of Working solution
V = Volume

PROB
• You are provided with 50X TAE Buffer. From that have to prepare 1x TAE for 300ml.
How?!!

CALCULATION
C1*V1 = C2*V2
50x * V1 = 1X * 300ml
V1 = 1X * 300/50x
V1 = 6 ml

RESULT
• Have to take 6 ml of 50xTAE and make up the volume till 300 ml with
Deionized water

13-07-2021
Dr.SS

13-07-2021
Dr.SS
sivasan91@gmail.com

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Stock solution calculation

1. STOCK SOLUTION CALCULATION Dr. S. SIVASANKARA NARAYANI

2. FORMULA • C1V1 = C2V2 C1 = Concentration of the stock solution C2 = Concentration of Working solution V = Volume

3. PROB • You are provided with 50X TAE Buffer. From that have to prepare 1x TAE for 300ml. How?!!

4. CALCULATION C1*V1 = C2*V2 50x * V1 = 1X * 300ml V1 = 1X * 300/50x V1 = 6 ml

5. RESULT • Have to take 6 ml of 50xTAE and make up the volume till 300 ml with Deionized water

6. 13-07-2021 Dr.SS

7. 13-07-2021 Dr.SS sivasan91@gmail.com