When animals die, muscles stiffen in rigor mortis because 1. actin and myosin cannot bind without ATP. 2. actin and myosin cannot separate without ATP. 3. ATP forms cross-bridges between filaments. Solution Answer : The correct answer is 2 (actin and myosin cannot separate without ATP).Explanation : After the death, ATP synthesis stops as breathing stops. However, so the crossbridges cannot detach from actin. The resulting condition, in which muscles are in a state of rigidity( cannot contract or stretch) is called rigor mortis..