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Pet

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Shahrzad Mirbaha
Shahrzad Mirbaha

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PET(Paired End di-tags)
PET cDNA cDNA 3 , 5  18bp  PET 
PET  GIS – PET CHIP – PET MS-PET 
GIS-PET fl-cDNA  GIS-PET 
GIS-PET GIS full-length cDNA (pGIS4) biotinylated CapTrapper GIS-PET  (flc-DNA) 
GIS-PET PET PET mRNA Cap Trapper 
cDNA mRNA diol 5Me-dcTP cDNA GSU1 A     
 cDNA cDNA ,TCCGAC PET cDNA NOT1   Mme1  T4   
3 CDNA Cap – Trapper  diol mRNA cap 5Me-dcTP   STREPTAVIDIN A GSU1 CTGGAG
STEP1 AAAAAAAA CTGGAG STEP2 STEP3 T16 GCGGCCGC :GIS N5 GCGGCCGC:GIS N6
STEP5 NOT1 NOT1 M/B/S STEP6 PGIS4 GIS-full length cDNAlibrary
TCCGAC STEP7 Mme1 Mme1 digestion and end polish using T4 polymerase AA XX NN TT 20bp Self-ligate , propagate in bacteria Extract single- PET plasmid DNA STEP8 18bp GIS single PET library AA M/B/S
STEP9 release and purifry 50-bp PET b M b Concanate PETs into vectore STEP10 GIS PET library
STEP11 Sequencing and mapping GIS PETs to genome Gen1 Gen2 Gen3
PET transcriptome Genome annotation   
 

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