Cytoskeleton PPT BY Dr.Pinky Diwedi

1. Cytoskeleton
M.Sc.IIsem.
Unit-I
Dr. Pinky Dwivedi

2. Providing structural support to the cell,
the cytoskeleton also functions in cell
motility and regulation

3. Structural Support
• Mechanical support
– Maintains shape
• Fibers act like a geodesic dome to
stabilize and balance opposing forces
• Provides anchorage for organelles
• Dynamic
– Dismantles in one spot and reassembles in
another to change cell shape

4. • Introduction
• The cytoskeleton is a network of fibers
extending throughout the cytoplasm.
• The cytoskeleton
organizes the
structures and
activities of
the cell.

5. • The cytoskeleton also plays a major role in
cell motility.
– This involves both changes in cell location and
limited movements of parts of the cell.
• The cytoskeleton interacts with motor
proteins.
– In cilia and flagella motor proteins pull
components
of the cytoskeleton past each other.
– This is also true
in muscle cells.
Fig. 7.21a

6. • Motor molecules also carry vesicles or
organelles to various destinations along
“monorails’ provided by the cytoskeleton.
• Interactions of motor proteins and the
cytoskeleton circulates materials within a
cell via streaming.
• Recently, evidence is accumulating th
• at the cytoskeleton may
transmit mechanical
signals that rearrange
the nucleoli and
Fig. 7.21b

7. • There are three main types of fibers in the
cytoskeleton: microtubules,
microfilaments, and intermediate
filaments.

9. • Microtubules, the thickest fibers, are hollow rods
about 25 microns in diameter.
– Microtubule fibers are constructed of the
globular protein, tubulin, and they grow or
shrink as more tubulin molecules are added or
removed.
• They move chromosomes during cell division.
• Another function is
as tracks that guide
motor proteins
carrying organelles
to their destination.
Fig. 7.21b

10. • In many cells, microtubules grow out from a
centrosome near the nucleus.
– These microtubules resist compression to the
cell.

11. Fig. 7.22
• In animal cells, the centrosome has a pair of
centrioles, each with nine triplets of microtubules
arranged in a ring.
• During cell division the
centrioles replicate.

12. • Microtubules are the central structural
supports in cilia and flagella.
– Both can move unicellular and small
multicellular organisms by propelling water
past the organism.
– If these structures are anchored in a large
structure, they move fluid over a surface.
• For example, cilia sweep mucus carrying trapped
debris from the lungs.
Fig. 7.2

13. • Cilia usually occur in large numbers on the
cell surface.
– They are about 0.25 microns in diameter and
2-20 microns long.
• There are usually just one or a few flagella
per cell.
– Flagella are the same width as cilia, but 10-200
microns long.

14. • A flagellum has an undulatory movement.
– Force is generated parallel to the flagellum’s
axis.
Fig. 7.23a

15. Fig. 7.23b
• Cilia move more like oars with alternating
power and recovery strokes.
– They generate force perpendicular to the cilia’s
axis.

16. • In spite of their differences, both cilia and
flagella have the same ultrastructure.
– Both have a core of microtubules sheathed by
the plasma membrane.
– Nine doublets of microtubules arranged around
a pair at the center, the “9 + 2” pattern.
– Flexible “wheels” of proteins connect outer
doublets to each other and to the core.
– The outer doublets are also connected by
motor proteins.
– The cilium or flagellum is anchored in the cell
by a basal body, whose structure is identical
to a centriole.

17. Fig. 7.24

18. • The bending of cilia and flagella is driven by
the arms of a motor protein, dynein.
– Addition to dynein of a phosphate group from
ATP and its removal causes conformation
changes in the protein.
– Dynein arms alternately
grab, move, and release
the outer microtubules.
– Protein cross-links limit
sliding and the force is
expressed as bending.
Fig. 7.25

19. • Microfilaments, the thinnest class of the
cytoskeletal fibers, are solid rods of the
globular protein actin.
– An actin microfilament consists of a twisted
double chain of actin subunits.
• Microfilaments are designed to resist
tension.
• With other proteins, they form a three-
dimensional network just inside the plasma
membrane.

20. Fig. 7.26 The shape of the
microvilli in this intestinal cell
are supported by microfilaments,
anchored to a network of
intermediate filaments.

21. • In muscle cells, thousands of actin filaments are
arranged parallel to one another.
• Thicker filaments, composed of a motor protein,
myosin, interdigitate with the thinner actin fibers.
– Myosin molecules walk along the actin filament,
pulling stacks of actin fibers together and
shortening
the cell.
Fig. 7.21a

22. • In other cells, these actin-myosin aggregates are less
organized but still cause localized contraction.
– A contracting belt of microfilaments divides the
cytoplasm of animals cells during cell division.
– Localized contraction also drives amoeboid movement.
• Pseudopodia, cellular extensions, extend and
contract through the reversible assembly and
contraction of actin subunits into microfilaments.
Fig. 7.21b

23. • In plant cells (and others), actin-myosin
interactions and sol-gel transformations
drive cytoplasmic streaming.
– This creates a circular flow of cytoplasm in the
cell.
– This speeds the distribution of materials within
the cell.
Fig. 7.21c

24. • Intermediate filaments,
intermediate in size at 8 -
12 nanometers, are
specialized for bearing
tension.
– Intermediate filaments
are built from a diverse
class of subunits from a
family of proteins called
keratins.
• Intermediate filaments are
more permanent fixtures of
the cytoskeleton than are
the other two classes.
• They reinforce cell shape
and fix organelle location. Fig. 7.26