Experiment
Region
Tissue expressed
Conclusion
1
A
B
C
LIVER
KIDNEY
-
X, Y and Z protiens are interacting with regions A, B and C, respectively. Expression is observed
only in liver and kidney, indicating that interaction of any one of these proteins is blocking the
expression of this gene in heart.
2
-
-
-
-
-
HEART
None of the promoter region is present for binding site and still expression is observed in heart.
This confirms that any one of these regions are blocking the expression of this gene in heart.
3
A
-
-
-
KIDNEY
HEART
Region B and C are mutated. Expression is observed in kidney and heart, indicating that A is not
blocking gene expression in heart, but gives specific expression in kidney. Interaction of A-X is
essential for kidney-specific expression.
4
-
B
-
LIVER
-
HEART
Region A and C are mutated. Expression is observed in liver and heart, indicating that B is not
blocking gene expression in heart, but gives specific expression in liver. Interaction of B-Y is
essential for liver-specific expression.
5
-
-
C
-
-
-
Region A and B are mutated. Expression is not observed in any tissue, indicating that C is
blocking gene expression in heart. Interaction of C-Z is essential for blocking the gene
expression in heart.
6
A
B
-
LIVER
KIDNEY
HEART
Region C is mutated. Expression is observed in all tissues, confirming that A and B are not
blocking gene expression in heart.
7
A
-
C
-
KIDNEY
-
Region B is mutated. Expression is observed in only kidney, confirming that C is blocking gene
expression in heart.
8
-
B
C
LIVER
-
-
Region A is mutated. Expression is observed in only liver, confirming that C is blocking gene
expression in heart.
From the data given, it is clear that the region C is important for heart-specific expression of this
gene. Therefore protein Z is present in heart.
Experiment
Region
Tissue expressed
Conclusion
1
A
B
C
LIVER
KIDNEY
-
X, Y and Z protiens are interacting with regions A, B and C, respectively. Expression is
observed only in liver and kidney, indicating that interaction of any one of these proteins is
blocking the expression of this gene in heart.
2
-
-
-
-
-
HEART
None of the promoter region is present for binding site and still expression is observed in heart.
This confirms that any one of these regions are blocking the expression of this gene in heart.
3
A
-
-
-
KIDNEY
HEART
Region B and C are mutated. Expression is observed in kidney and heart, indicating that A is not
blocking gene expression in heart, but gives specific expression in kidney. Interaction of A-X is
essential for kidney-specific expression.
4
-
B
-
LIVER
-
HEART
Region A and C are mutated. Expression is observed in liver and heart, indicating that B is not
blocking gene expression in heart, but gives specific expression in liver. Interaction of B-Y is
essential for liver-specific expression.
5
-
-
C
-
-
-
Region A and B are mutated. Expression is not observed in any tissue, indicating that C is
blocking gene expression in heart. Interaction of.
1. Experiment
Region
Tissue expressed
Conclusion
1
A
B
C
LIVER
KIDNEY
-
X, Y and Z protiens are interacting with regions A, B and C, respectively. Expression is observed
only in liver and kidney, indicating that interaction of any one of these proteins is blocking the
expression of this gene in heart.
2
-
-
-
-
-
HEART
None of the promoter region is present for binding site and still expression is observed in heart.
This confirms that any one of these regions are blocking the expression of this gene in heart.
3
A
-
-
-
KIDNEY
HEART
Region B and C are mutated. Expression is observed in kidney and heart, indicating that A is not
blocking gene expression in heart, but gives specific expression in kidney. Interaction of A-X is
essential for kidney-specific expression.
4
-
2. B
-
LIVER
-
HEART
Region A and C are mutated. Expression is observed in liver and heart, indicating that B is not
blocking gene expression in heart, but gives specific expression in liver. Interaction of B-Y is
essential for liver-specific expression.
5
-
-
C
-
-
-
Region A and B are mutated. Expression is not observed in any tissue, indicating that C is
blocking gene expression in heart. Interaction of C-Z is essential for blocking the gene
expression in heart.
6
A
B
-
LIVER
KIDNEY
HEART
Region C is mutated. Expression is observed in all tissues, confirming that A and B are not
blocking gene expression in heart.
7
A
-
C
-
KIDNEY
-
Region B is mutated. Expression is observed in only kidney, confirming that C is blocking gene
expression in heart.
3. 8
-
B
C
LIVER
-
-
Region A is mutated. Expression is observed in only liver, confirming that C is blocking gene
expression in heart.
From the data given, it is clear that the region C is important for heart-specific expression of this
gene. Therefore protein Z is present in heart.
Experiment
Region
Tissue expressed
Conclusion
1
A
B
C
LIVER
KIDNEY
-
X, Y and Z protiens are interacting with regions A, B and C, respectively. Expression is
observed only in liver and kidney, indicating that interaction of any one of these proteins is
blocking the expression of this gene in heart.
2
-
-
-
-
-
HEART
None of the promoter region is present for binding site and still expression is observed in heart.
This confirms that any one of these regions are blocking the expression of this gene in heart.
3
A
4. -
-
-
KIDNEY
HEART
Region B and C are mutated. Expression is observed in kidney and heart, indicating that A is not
blocking gene expression in heart, but gives specific expression in kidney. Interaction of A-X is
essential for kidney-specific expression.
4
-
B
-
LIVER
-
HEART
Region A and C are mutated. Expression is observed in liver and heart, indicating that B is not
blocking gene expression in heart, but gives specific expression in liver. Interaction of B-Y is
essential for liver-specific expression.
5
-
-
C
-
-
-
Region A and B are mutated. Expression is not observed in any tissue, indicating that C is
blocking gene expression in heart. Interaction of C-Z is essential for blocking the gene
expression in heart.
6
A
B
-
LIVER
KIDNEY
HEART
Region C is mutated. Expression is observed in all tissues, confirming that A and B are not
5. blocking gene expression in heart.
7
A
-
C
-
KIDNEY
-
Region B is mutated. Expression is observed in only kidney, confirming that C is blocking gene
expression in heart.
8
-
B
C
LIVER
-
-
Region A is mutated. Expression is observed in only liver, confirming that C is blocking gene
expression in heart.
Solution
Experiment
Region
Tissue expressed
Conclusion
1
A
B
C
LIVER
KIDNEY
-
X, Y and Z protiens are interacting with regions A, B and C, respectively. Expression is observed
only in liver and kidney, indicating that interaction of any one of these proteins is blocking the
expression of this gene in heart.
6. 2
-
-
-
-
-
HEART
None of the promoter region is present for binding site and still expression is observed in heart.
This confirms that any one of these regions are blocking the expression of this gene in heart.
3
A
-
-
-
KIDNEY
HEART
Region B and C are mutated. Expression is observed in kidney and heart, indicating that A is not
blocking gene expression in heart, but gives specific expression in kidney. Interaction of A-X is
essential for kidney-specific expression.
4
-
B
-
LIVER
-
HEART
Region A and C are mutated. Expression is observed in liver and heart, indicating that B is not
blocking gene expression in heart, but gives specific expression in liver. Interaction of B-Y is
essential for liver-specific expression.
5
-
-
C
-
-
-
7. Region A and B are mutated. Expression is not observed in any tissue, indicating that C is
blocking gene expression in heart. Interaction of C-Z is essential for blocking the gene
expression in heart.
6
A
B
-
LIVER
KIDNEY
HEART
Region C is mutated. Expression is observed in all tissues, confirming that A and B are not
blocking gene expression in heart.
7
A
-
C
-
KIDNEY
-
Region B is mutated. Expression is observed in only kidney, confirming that C is blocking gene
expression in heart.
8
-
B
C
LIVER
-
-
Region A is mutated. Expression is observed in only liver, confirming that C is blocking gene
expression in heart.
From the data given, it is clear that the region C is important for heart-specific expression of this
gene. Therefore protein Z is present in heart.
Experiment
Region
Tissue expressed
Conclusion
8. 1
A
B
C
LIVER
KIDNEY
-
X, Y and Z protiens are interacting with regions A, B and C, respectively. Expression is
observed only in liver and kidney, indicating that interaction of any one of these proteins is
blocking the expression of this gene in heart.
2
-
-
-
-
-
HEART
None of the promoter region is present for binding site and still expression is observed in heart.
This confirms that any one of these regions are blocking the expression of this gene in heart.
3
A
-
-
-
KIDNEY
HEART
Region B and C are mutated. Expression is observed in kidney and heart, indicating that A is not
blocking gene expression in heart, but gives specific expression in kidney. Interaction of A-X is
essential for kidney-specific expression.
4
-
B
-
LIVER
-
HEART
9. Region A and C are mutated. Expression is observed in liver and heart, indicating that B is not
blocking gene expression in heart, but gives specific expression in liver. Interaction of B-Y is
essential for liver-specific expression.
5
-
-
C
-
-
-
Region A and B are mutated. Expression is not observed in any tissue, indicating that C is
blocking gene expression in heart. Interaction of C-Z is essential for blocking the gene
expression in heart.
6
A
B
-
LIVER
KIDNEY
HEART
Region C is mutated. Expression is observed in all tissues, confirming that A and B are not
blocking gene expression in heart.
7
A
-
C
-
KIDNEY
-
Region B is mutated. Expression is observed in only kidney, confirming that C is blocking gene
expression in heart.
8
-
B
C
LIVER
10. -
-
Region A is mutated. Expression is observed in only liver, confirming that C is blocking gene
expression in heart.