Heat resistant probiotic food addotive that could be used in different food products.

1. presents
Functional properties of Thermostable lactic acid- forming bacteria (B. coagulans)
Dr. Maryam Tajabadi Ebrahimi
Iranian Society of Probiotic and
Functional Food

2. 2
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3. 1014 micro-organisms,
>400 differentes species
stomach
Jejunum
Ileum
Colon with appendix
Duodenum
102 to 103
<105
104 to 107
109 to 1012
Lactobacilli
Streptococci Lactobacilli
Enterobacteria Enterococcus Faesalis Bacteroides Bifidobacteria Peptococcus Raminococcus Clostridia Lactobacilli
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6. 2. Moreover, we also consume antibacterial substances ranging from vinegar to antibiotics
1. The changing food habits and lifestyle force us to take processed and sterile food, which affects our access to, and colonization, by certain type of bacteria.
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7. 400+
Species
of Prpbiotics
In Your Body
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“Death sits in the bowels; a bad digestion is the root of all evil” - Hippocrates, ca. 400 BC
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First described by Metchnikoff in 1908“Live microbial food ingredients that alter the microflora and confer health benefit”
WHO definition(2001): “Live micro-organisms which, when administered in adequate amounts, confer a health benefit on the host”

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Prebiotic
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Number of publications/year
Awareness of the terms are moving into "mainstream"… with 3 of 5 consumers now aware of the term probiotics
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11. Probiotic Supplements
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Probiotic Functional foods

12. Dairy
Non-Dairy
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13. Dairy 38%
Bakery&cereal 23%
Beverage 12%
Meat, Fish&egg 7%
Soya Products 6%
Fat& Oil 8%
Other 6%
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14. L. sporogenes (Bacillus coagulance)
Contain a large number of viable lactobacilli that retain viability during preparation in pharmaceutical dosage forms and during storage before consumption.
The spores are thermostable as against viable L. acidophilus cells which may not withstand spray drying.
Survive in gastric secretions and bile of the upper digestive tract and reach the intestine safely.
Settle in the digestive tract and produce enough lactic acid and other antagonistic substances to inhibit the growth of pathogenic bacteria.
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17. Bacillus coagulans has been added by the EFSA to their Qualified Presumption of Safety list approved for as GRAS by the U.S. Food and Drug Administration's Center
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20. •Maturation of mononuclear phagocytes toward both macrophage and dendritic cell
•T helper cell differentiation
•Generating T regulatory cells
•Cell wall & metabolites modulate inflammatory processes
•Induction of IL-10,
•Possible treatment of inflammatory bowel diseases
•Adjunct therapy for relieving symptoms of rheumatoid arthrite Bebsons et al. World J Gastroenterol 2012 April 28; 18(16): 1875-1883 Mandel et al. BMC Complementary and Alternative Medicine 2010, 10:1 Jensen et al. BMC Immunology 2010, 11:15
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21. •Improve bowel movement
•Defecation frequency
•Increase number of intestinal bifdobacteria
•Decrease intestinal clostridium perfringens
•Improvements in GSRS abdominal pain
•Concentrations of intestinal ammonia, indole and p-cresol decreased
•Improved abdominal pain and bloating in patients with IBS Phalguni Dutta et al. Tropical Medicine and International Health Kal man et al., BMC Gastroenterology 2009 Larysa Hun et al., Postgraduate Medicine, 2009 Katsutoshi Ara, et al., 2002, Microbial Ecology in Health and Disease
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22. •Decrease in serum insulin levels
•hs-crp,
•Uric acid
•Reduces levels of lipid profile
•Increase of glutathione
•Increase of TAC Z. Asemi et al., Clinical Nutrition, 2013 Z. Asemi et al., Lipid, 2013 Shakeri et al., Lipid, 2014 Tajabadi Ebrahimi et al., 2014, Ann Nutr Metab
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23. •Screening for Aflatoxin binding
•Alanin transferase (ALT)
•Aspartat aminotransferase (AST)
•Alkalin transferase (ALP)
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Control
Aflatoxin B1
Aflatoxin B1+ B. Coagulance
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•Take care of microbiota
•Probiotic can improve healthy microbiota
•B. coagulance is heat resistant probiotic - Immune modulation - Metabolic status in diabetic patients - Gut health
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26. (Dr. Michael L. McCann)
“Probiotics will be medicine
in the 21st century what antibiotics and microbiology were in the 20th”
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