For Eosin Methylene Blue Agar, what results would you be looking for? what are indicators?Theory Eosin methylene blue (EMB) agar is a complex (chemically EMB agar (Levine) is used for the isolation of fecal undefined), selective, and differential medium. It contains coliforms. It can be streaked for isolation or used in the digest of gelatin, lactose, and the dyes eosin Y and Membrane Filter Technique as discussed in Exercise 7-5. methylene blue. The gelatin provides nitrogen and organic carbon. Lactose is fermented ro acid end-products by coliforms such as Escherichia coli and Enterobacter aerogenes, whereas it is not by pathogens such as Proteus, You will spot inoculate one EMB agar plate and one Shigella, and Salmonella species. (See Fig. 4.5 for an _nutrient agar (NA) plate with four test organisms. The introduction to lactose fermentation.) NA plate will serve as a comparison for growth quality The purpose of the dyes is twofold: (1) they inhibit on the EMB agar plate. the growth of most Gram-positive organisms (some Enterococcus and Staphylococcus species are exeeptions), Materials and (2) they react with vigorous lactose fermenters whose acid end-products turn the growth dark purple or black. Per Student This dark growth is typical of Escherichia colt and is Lab coat usually accompanied by a green metallic sheen (Fig, 4.7). Disposable gloves Other, less-aggressive lactose fermenters, such as Chemical eye protection Enterobacter or Klebsiella species, produce colonies that can range from pink to dark purple on the medium. Lactose non-fermenters typically retain their normal color or take on the coloration of the medium. One NA plate Fresh broth cultures of these recommended organisms: - Enterobacter aerogenes - Escherichia coli - Providencia stuartiit - Stapbylococcus epidermidis In Medium Recipes Eosin Methylene Blue fgar (Levine) 4.7 Eosin Methylene Blue Agar (EMB) = This EMB plate was. PancreaticdigestofgelatinLactose DipotassiumphosphateMethyleneblueEosinYAgarDistilledordeionizedwater10.0g10.0g2.0g0.065g 0.4g15.0g1.0L inoculated with a Gram-positive coccus (center) and two members of the Enterobocteriaceoc, a coliform at the top and a noncoliform at the bottom. The Gram-positive coccus was inhibited by eosin and methylene blue dyes but still grew enough to forma thin film on the agar. Vigorous lactose fermentation by the coliform gave its growth. a shiny green appearance. The noncoliform was not inhibited by the dyes but also did not ferment the lactose, and so is a more natural color. EMB would normally be streaked for isolation, but you will spot inoculate pure cultures for the sole purpose of seeing what the different results look like.Lati 0nin 1 Wear a lab coar, gloves, and chemical eye protection 13thad se Louic, Mo: Mosby. 2014. when performing this procedure. Thinn, Washinction C-et al. Komemanj Color Athes amit Terthook of 2 Gently mix cach culture according to your lab's Diecmoneic Microtinlog. esth ed. Baleimore Lippinepa.

1. For Eosin Methylene Blue Agar, what results would you be looking for? what are
indicators?Theory Eosin methylene blue (EMB) agar is a complex (chemically EMB agar (Levine)
is used for the isolation of fecal undefined), selective, and differential medium. It contains
coliforms. It can be streaked for isolation or used in the digest of gelatin, lactose, and the dyes
eosin Y and Membrane Filter Technique as discussed in Exercise 7-5. methylene blue. The gelatin
provides nitrogen and organic carbon. Lactose is fermented ro acid end-products by coliforms
such as Escherichia coli and Enterobacter aerogenes, whereas it is not by pathogens such as
Proteus, You will spot inoculate one EMB agar plate and one Shigella, and Salmonella species.
(See Fig. 4.5 for an _nutrient agar (NA) plate with four test organisms. The introduction to lactose
fermentation.) NA plate will serve as a comparison for growth quality The purpose of the dyes is
twofold: (1) they inhibit on the EMB agar plate. the growth of most Gram-positive organisms (some
Enterococcus and Staphylococcus species are exeeptions), Materials and (2) they react with
vigorous lactose fermenters whose acid end-products turn the growth dark purple or black. Per
Student This dark growth is typical of Escherichia colt and is Lab coat usually accompanied by a
green metallic sheen (Fig, 4.7). Disposable gloves Other, less-aggressive lactose fermenters,
such as Chemical eye protection Enterobacter or Klebsiella species, produce colonies that can
range from pink to dark purple on the medium. Lactose non-fermenters typically retain their normal
color or take on the coloration of the medium. One NA plate Fresh broth cultures of these
recommended organisms: - Enterobacter aerogenes - Escherichia coli - Providencia stuartiit -
Stapbylococcus epidermidis In Medium Recipes Eosin Methylene Blue fgar (Levine) 4.7 Eosin
Methylene Blue Agar (EMB) = This EMB plate was. PancreaticdigestofgelatinLactose
DipotassiumphosphateMethyleneblueEosinYAgarDistilledordeionizedwater10.0g10.0g2.0g0.065g
0.4g15.0g1.0L inoculated with a Gram-positive coccus (center) and two members of the
Enterobocteriaceoc, a coliform at the top and a noncoliform at the bottom. The Gram-positive
coccus was inhibited by eosin and methylene blue dyes but still grew enough to forma thin film on
the agar. Vigorous lactose fermentation by the coliform gave its growth. a shiny green
appearance. The noncoliform was not inhibited by the dyes but also did not ferment the lactose,
and so is a more natural color. EMB would normally be streaked for isolation, but you will spot
inoculate pure cultures for the sole purpose of seeing what the different results look like.Lati 0nin 1
Wear a lab coar, gloves, and chemical eye protection 13thad se Louic, Mo: Mosby. 2014. when
performing this procedure. Thinn, Washinction C-et al. Komemanj Color Athes amit Terthook of 2
Gently mix cach culture according to your lab's Diecmoneic Microtinlog. esth ed. Baleimore
Lippinepat Williams iE standards. Wilkins, 2006 . 3 Using a permanene marker, divide the bottom
of cach plate into four sectors. 4 Label the plates with the organisms' names, your name, and the
date, Use the same positions for each specimen on both plates. 5 Spot inoculate (refer to
Appendix B, "Spot Inoculation of an Agar Plate," p. 601) the sectors on the EMB plate with the rest
organisms. 6. Repeat step 5 with the nutrient agar plate, Be sure to inoculate the specimens in the
secrors that correspond to their position on the EMB plate. 7. Invert and incubare the plate at 352
C for 24 to 48 hours; 8 Save or dispose of the original cultures as directed by your instructor. Lab
Two 1 Examine and compare the plates for color and quality of growth. 2. Record your results on
the dara sheet, page 257. Refer to Table 4-5 as needed. 3 Dispose of all plates in the appropriate
autoclave container when finished.