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Bacterial Growth and Metabolism Dr Kamran Afzal FCPS Microbiology
Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Types of growth ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Doubling time ,[object Object],[object Object],[object Object],[object Object],[object Object]
Growth phases in broth culture ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Lag phase ,[object Object],[object Object]
Exponential (Log) phase ,[object Object],[object Object]
Stationary phase ,[object Object]
Decline phase ,[object Object],[object Object]
Media for bacterial growth ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Types of culture media ,[object Object],[object Object],[object Object],[object Object],[object Object]
a. Simple media ,[object Object],[object Object],[object Object]
b. Enriched media ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
c. Selective media ,[object Object],[object Object],[object Object],[object Object],[object Object]
d. Differential media ,[object Object],[object Object],[object Object]
e. Enrichment media ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Identification of microbes ,[object Object],[object Object],[object Object],[object Object],[object Object]
Bacterial Physiology Nutritional types Physical conditions required for growth Bacterial metabolism Adaptive response Bacterial viability
1. Nutritional types ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Nutritional types
2. Physical conditions ,[object Object],[object Object],[object Object]
a. Growth atmosphere Descriptive term Property Example Growth atmosphere Strict (Obligate) aerobe Requires O 2 Pseudomonas aeruginosa Strict (Obligate) anaerobe Will not tolerate O 2 Bacteroides fragilis Facultative anaerobe Aerobe, can grow anaerobically Staphylococci,  Escherichia coli Aerotolerant anaerobe Anaerobe, can tolerate O 2 Clostridium perfringens Micro-aerophilic Prefers reduced O 2 Campylobacter spp, Helicobacter spp Capnophilic Prefers increased CO 2 Neisseria spp
 
b. Growth temperature Descriptive term Property Example Growth temperature Psychrophilic Low temp <10 0 C Flavobacterium spp Thermophilic High temp >60 0 C B. stearothermophilus Mesophilic 20-40 0 C Most bacterial pathogens
3. Bacterial metabolism ,[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Basic chemical reactions ,[object Object],[object Object],[object Object]
Building   and breaking down molecules ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Anabolism ,[object Object]
Catabolism ,[object Object],[object Object],[object Object]
Oxidation - Reduction reactions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
ATP production and Energy storage ,[object Object],[object Object]
Bacterial respiration ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Aerobic cellular respiration ,[object Object],[object Object]
Using oxygen in metabolism creates toxic waste ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
4. Adaptive responses ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
5. Bacterial viability ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
 

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BACTERIAL GROWTH

  • 1. Bacterial Growth and Metabolism Dr Kamran Afzal FCPS Microbiology
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  • 19. Bacterial Physiology Nutritional types Physical conditions required for growth Bacterial metabolism Adaptive response Bacterial viability
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  • 25. a. Growth atmosphere Descriptive term Property Example Growth atmosphere Strict (Obligate) aerobe Requires O 2 Pseudomonas aeruginosa Strict (Obligate) anaerobe Will not tolerate O 2 Bacteroides fragilis Facultative anaerobe Aerobe, can grow anaerobically Staphylococci, Escherichia coli Aerotolerant anaerobe Anaerobe, can tolerate O 2 Clostridium perfringens Micro-aerophilic Prefers reduced O 2 Campylobacter spp, Helicobacter spp Capnophilic Prefers increased CO 2 Neisseria spp
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  • 27. b. Growth temperature Descriptive term Property Example Growth temperature Psychrophilic Low temp <10 0 C Flavobacterium spp Thermophilic High temp >60 0 C B. stearothermophilus Mesophilic 20-40 0 C Most bacterial pathogens
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Editor's Notes

  1. Enterobacteriacea Metabolic capabilities
  2. Synthesized Break down
  3. Oxidation Reduction
  4. Photosynthesis Cellular Respiration