Locate an article where the technique of Polymerase chain reaction (PCR) is used.Describe the
technique briefly based on your study material, and explain how this technique provided the
authors with the key information for their results and conclusions.
Solution
This article fairly explain the PCR technique.
\"Activation of Wnt/beta-catenin/Tcf signaling pathway in human astrocytomas\"
In this article Semi -Quantitative PCR technique is used for the analysis of mRNA levels of -
Catenin/Tcf Signaling components.
Initially total RNA isolated from Freezed brain Astrocyte tissue. From thios RNA, cDNA
synthecized using Takara cDNA reverse transcriptase kit. Then the produced cDNA is amplified
with different primers of the -Catenin/Tcf Signaling components that include Dvl-1, Dvl-2, Dvl-
3, beta-catenin, c-myc and cyclin D1.
The mRNA levels were positively correlated with the histological malignancy. Our studies tend
to suggest that Wnt/beta-catenin/Tcf signaling pathway is implicated in malignancy of
astrocytomas.
Python Notes for mca i year students osmania university.docx
Locate an article where the technique of Polymerase chain reaction (.pdf
1. Locate an article where the technique of Polymerase chain reaction (PCR) is used.Describe the
technique briefly based on your study material, and explain how this technique provided the
authors with the key information for their results and conclusions.
Solution
This article fairly explain the PCR technique.
"Activation of Wnt/beta-catenin/Tcf signaling pathway in human astrocytomas"
In this article Semi -Quantitative PCR technique is used for the analysis of mRNA levels of -
Catenin/Tcf Signaling components.
Initially total RNA isolated from Freezed brain Astrocyte tissue. From thios RNA, cDNA
synthecized using Takara cDNA reverse transcriptase kit. Then the produced cDNA is amplified
with different primers of the -Catenin/Tcf Signaling components that include Dvl-1, Dvl-2, Dvl-
3, beta-catenin, c-myc and cyclin D1.
The mRNA levels were positively correlated with the histological malignancy. Our studies tend
to suggest that Wnt/beta-catenin/Tcf signaling pathway is implicated in malignancy of
astrocytomas
