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In a plaque assay, 100uL of phage stock solution containing 5.7 * 102 pfu/Ml is mixed with 0.9 mL
of solution containing host bacteria. Further decimal serial dilutions of this mixture are made. 1 mL
of each dilution is added to a nutrient agar plate. What is the average number of plaques you
anticipate to count for phage samples that were incubated on the following dilution plates. 1) 10-5
dilution plate 2) 10-4 dilution plate

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Plaque Assay Phage Dilution Plate Counts

  • 1. In a plaque assay, 100uL of phage stock solution containing 5.7 * 102 pfu/Ml is mixed with 0.9 mL of solution containing host bacteria. Further decimal serial dilutions of this mixture are made. 1 mL of each dilution is added to a nutrient agar plate. What is the average number of plaques you anticipate to count for phage samples that were incubated on the following dilution plates. 1) 10-5 dilution plate 2) 10-4 dilution plate