1. DNA ReplicationDNA Replication

2. DNA ReplicationDNA Replication
Replication = DNA copies itselfReplication = DNA copies itself exactlyexactly
(Occurs within the nucleus)(Occurs within the nucleus)
Any mistake in copying =Any mistake in copying = mutationmutation
DNA mutation = chromosomal mutationDNA mutation = chromosomal mutation

3. A. Basic Facts of DNAA. Basic Facts of DNA
ReplicationReplication
1.1. ComplementaryComplementary
base pairingbase pairing
makesmakes
replication possiblereplication possible
CC -- GG
AA -- TT

4. A. Basic Facts of DNAA. Basic Facts of DNA
ReplicationReplication
2. One side of DNA2. One side of DNA
molecule ismolecule is
aa
templatetemplate
forfor
makingmaking
thethe
other sideother side
(strand)(strand) ?’s 1-3

5. B. Process of DNA ReplicationB. Process of DNA Replication
1.1. Uncoil & unzip DNA moleculeUncoil & unzip DNA molecule
Enzyme (Enzyme (--asease)) breaksbreaks
weakweak
Hydrogen BondHydrogen Bond
between basesbetween bases

6. B. Process of DNA ReplicationB. Process of DNA Replication
2.2. Enzyme brings in complementary NEnzyme brings in complementary N--basesbases
DNA Polymerase

7. B. Process of DNA ReplicationB. Process of DNA Replication
3.3. Insert NInsert N--basesbases
DNA Replication TutorialDNA Replication Tutorial
(Go here for on(Go here for on--youryour--own learning/review)own learning/review)
trc.ucdavis.edu/.../week5/06dnareplication.html

8. ReplicationReplication MoovieMoovie
CC -- GG
AA -- TT

9. C.C. SemiSemi--conservative replicationconservative replication
1.1. Each new DNAEach new DNA
moleculemolecule
containscontains
one old strandone old strand
&&
one new strandone new strand
?’s 4-6

11. DNA vs. RNADNA vs. RNA
DNADNA RNARNA
Sugar =Sugar = deoxyribosedeoxyribose Sugar =Sugar = riboseribose
DoubleDouble--strandedstranded
moleculemolecule
SingleSingle--strandedstranded
moleculemolecule
ThymineThymine bonds withbonds with
adenineadenine
UracilUracil instead of thymineinstead of thymine

12. DNA vs. RNADNA vs. RNA
DNADNA RNARNA
NuclearNuclear DNADNA
MitochondrialMitochondrial DNADNA
ChloroplastChloroplast DNADNA
mRNAmRNA = messenger= messenger
tRNAtRNA = transfer= transfer
rRNArRNA = ribosomal= ribosomal
Nuclear DNANuclear DNA nevernever
leaves the nucleusleaves the nucleus
Assembled in nucleus,Assembled in nucleus,
moves to cytoplasmmoves to cytoplasm
((leaves the nucleusleaves the nucleus))

13. DNADNA vs.vs. RNARNA
?’s 7-12