Immune electron microscopy is a variation of electron microscopy. It is applied for diagnosis of many viral infections. A difficult procedure, IEM was developed as a diagnostic aid for detecting and identifying transmissible gastroenteritis virus and rotavirus (reovirus-like agent) in fecal and intestinal contents from cases of gastroenteritis in young pigs. IEM is one of the fastest and most sensitive methods for the detection and diagnosis of viruses. This technique is based on formation of immune complexes of the virus with its corresponding antibody.

1. ImmunoelectronMicroscopy.
Undertheguidance OF-
Jyotirawat
(ASSIST.Professor)
PresentedBy-
Mo.AsadFarooqui
B.Pharm.-4thYear
CHANDRASHEKHARSINGH COLLEGE OF PHARMACY KOILAHA
PURAMUFTI KAUSHAMBI.
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9. GOLDPARTICLESASAPROBE
Goldbecamethemostreliablechoiceforimmunogoldlabelingdueto
LargespecificsurfaceareaGoodbiocompatibilityandHighelectron
density,whichofferseasydetection.
Thesizeofgoldparticlesusedforimmunogoldlabelingvariesfrom1to
40µm,chosenaccordingtothetypeoflabelingtechniquesemployed
Detectingmultipleantigenswithinacellmayrequiretheselectiveuseof
differentsizesofgoldparticles.
Smallergoldparticles(2nmor5nm)produceahigherlabelingintensity
andlowersterichindrance.Largerparticlesizes(10nmormore)reduce
thepotentiallabelingintensityduetotheirsheersizebutaremoreeasily
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12. 1-https://en.wikipedia.org/wiki/Immune_electron_microscopy
Reference
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