Week 7 Assignment 3 Submission . Assignment 3: Constitutional Rights Research, review and analyze Korb v. Raytheon, 707 F.Supp. 63 (D.Mass. 1989). Based on your research and the case, write a 5-6 page paper in which you: 1.Analyze and evaluate each case independently by providing the following (about two paragraphs per case): •Facts of the case •Issues •Rule 2.Analyze and explain the challenges with freedom of speech. 3.Analyze and explain any challenges with freedom of information. 4.Analyze and explain any challenges with employment law. 5.Analyze and discuss the public perceptions of Raytheon and its influence with the Department of Defense. 6.Analyze and discuss any fraud or misrepresentation on either side of the case. 7.Provide at least four (4) additional court cases that support your analysis. Your assignment must: •Be typed, double spaced, using Times New Roman font (size 12), with one-inch margins on all sides; references must follow APA or school-specific format. Check with your professor for any additional instructions. •Include a cover page containing the title of the assignment, the student’s name, the professor’s name, the course title, and the date. The cover page and the reference page are not included in the required page length. The specific course learning outcomes associated with this assignment are: •Recommend how the legal system addresses critical issues faced by public officials to include freedom of the press, funding of government contracts, separation of church and state, public ethics law, hiring lawyers, and handling civil litigation. •Evaluate legal subjects relevant to public administration to include property, government contracts, employment, and torts. •Relate the administrative process, constitutional and statutory requirements, to the scope of judicial review of administrative agency decisions. •Assess legal decisions related to the administration of public goods. •Use technology and information resources to research issues in constitution and administrative law. •Write clearly and concisely about issues in constitution and administrative law using proper writing mechanics. Grading for this assignment will be based on answer quality, logic/organization of the paper, and language and writing skills, using the following rubric found here. PLEASE FOLLOW RUBRIC Points: 370 Assignment 3: Constitutional Rights Criteria Unacceptable Below 70% F Fair 70-79% C Proficient 80-89% B Exemplary 90-100% A 1. Analyze and evaluate case 1, including the facts of the case, issues, and rule. Weight: 12% Did not submit or incompletely analyzed and evaluated case 1, including the facts of the case, issues, and rule. Partially analyzed and evaluated case 1, including the facts of the case, issues, and rule. Satisfactorily analyzed and evaluated case 1, including the facts of the case, issues, and rule. Thoroughly analyzed and evaluated case 1, including the facts of the case, issues, and rule. 2. Analyze and evaluate case 2,.

1. Week 7 Assignment 3 Submission .
Assignment 3: Constitutional Rights
Research, review and analyze Korb v. Raytheon, 707 F.Supp. 63
(D.Mass. 1989). Based on your research and the case, write a 5-
6 page paper in which you:
1.Analyze and evaluate each case independently by providing
the following (about two paragraphs per case):
•Facts of the case
•Issues
•Rule
2.Analyze and explain the challenges with freedom of speech.
3.Analyze and explain any challenges with freedom of
information.
4.Analyze and explain any challenges with employment law.
5.Analyze and discuss the public perceptions of Raytheon and
its influence with the Department of Defense.
6.Analyze and discuss any fraud or misrepresentation on either
side of the case.
7.Provide at least four (4) additional court cases that support
your analysis.
Your assignment must:
•Be typed, double spaced, using Times New Roman font (size
12), with one-inch margins on all sides; references must follow
APA or school-specific format. Check with your professor for
any additional instructions.
•Include a cover page containing the title of the assignment, the
student’s name, the professor’s name, the course title, and the
date. The cover page and the reference page are not included in
the required page length.
The specific course learning outcomes associated with this
assignment are:

2. •Recommend how the legal system addresses critical issues
faced by public officials to include freedom of the press,
funding of government contracts, separation of church and state,
public ethics law, hiring lawyers, and handling civil litigation.
•Evaluate legal subjects relevant to public administration to
include property, government contracts, employment, and torts.
•Relate the administrative process, constitutional and statutory
requirements, to the scope of judicial review of administrative
agency decisions.
•Assess legal decisions related to the administration of public
goods.
•Use technology and information resources to research issues in
constitution and administrative law.
•Write clearly and concisely about issues in constitution and
administrative law using proper writing mechanics.
Grading for this assignment will be based on answer quality,
logic/organization of the paper, and language and writing skills,
using the following rubric found here.
PLEASE FOLLOW RUBRIC
Points: 370
Assignment 3: Constitutional Rights
Criteria
Unacceptable
Below 70% F
Fair
70-79% C
Proficient
80-89% B
Exemplary
90-100% A
1. Analyze and evaluate case 1, including the facts of the case,
issues, and rule.
Weight: 12%
Did not submit or incompletely analyzed and evaluated case 1,
including the facts of the case, issues, and rule.

3. Partially analyzed and evaluated case 1, including the facts of
the case, issues, and rule.
Satisfactorily analyzed and evaluated case 1, including the facts
of the case, issues, and rule.
Thoroughly analyzed and evaluated case 1, including the facts
of the case, issues, and rule.
2. Analyze and evaluate case 2, including the facts of the case,
issues, and rule.
Weight: 12%
Did not submit or incompletely analyzed and evaluated case 2,
including the facts of the case, issues, and rule.
Partially analyzed and evaluated case 2, including the facts of
the case, issues, and rule.
Satisfactorily analyzed and evaluated case 2, including the facts
of the case, issues, and rule.
Thoroughly analyzed and evaluated case 2, including the facts
of the case, issues, and rule.
3. Analyze and explain the challenges with freedom of speech.
Weight: 12%
Did not submit or incompletely analyzed and explained the
challenges with freedom of speech.
Partially analyzed and explained the challenges with freedom of
speech.
Satisfactorily analyzed and explained the challenges with
freedom of speech.
Thoroughly analyzed and explained the challenges with freedom
of speech.
4. Analyze and explain any challenges with freedom of
information.
Weight: 12%
Did not submit or incompletely analyzed and explained any
challenges with freedom of information.
Partially analyzed and explained any challenges with freedom of
information.
Satisfactorily analyzed and explained any challenges with
freedom of information.

4. Thoroughly analyzed and explained any challenges with
freedom of information.
5. Analyze and explain any challenges with employment law.
Weight: 12%
Did not submit or incompletely analyzed and explained any
challenges with employment law.
Partially analyzed and explained any challenges with
employment law.
Satisfactorily analyzed and explained any challenges with
employment law.
Thoroughly analyzed and explained any challenges with
employment law.
6. Analyze and discuss the public perceptions of Raytheon and
its influence with the Department of Defense.
Weight: 13%
Did not submit or incompletely analyzed and discussed the
public perceptions of Raytheon and its influence with the
Department of Defense.
Partially analyzed and discussed the public perceptions of
Raytheon and its influence with the Department of Defense.
Satisfactorily analyzed and discussed the public perceptions of
Raytheon and its influence with the Department of Defense.
Thoroughly analyzed and discussed the public perceptions of
Raytheon and its influence with the Department of Defense.
7. Analyze and discuss any fraud or misrepresentation on either
side of the case.
Weight: 12%
Did not submit or incompletely analyzed and discussed any
fraud or misrepresentation on either side of the case.
Partially analyzed and discussed any fraud or misrepresentation
on either side of the case.
Satisfactorily analyzed and discussed any fraud or
misrepresentation on either side of the case.
Thoroughly analyzed and discussed any fraud or
misrepresentation on either side of the case.
8. Clarity and writing mechanics

5. Weight: 10%
More than 6 errors present
5-6 errors present
3-4 errors present
0-2 errors present
9. Research and cite at least four (4) court cases that support the
analysis.
Weight: 5%
Researched and cited no court cases that support the analysis.
Researched and cited one or two (1 or 2) court cases that
support the analysis.
Researched and cited three (3) court cases that support the
analysis.
Researched and cited at least four (4) court cases that support
the
Oral microbiome and diabetes and obesity
Background: Diabetes and obesity are among the leading health
problems in the US. Oral microbiome may play roles in
maintaining systemic health including diabetes and obesity,
through pathogen inhibition, immune regulation, nutrition
absorption and absorption, and as a portal for bacteria to gain
access into the respiratory and digestive tracts. However, direct
evidence linking oral microbiome and diabetes and obesity is
lacking.
Methods: We conducted a case-control study nested from the
Southern Community Cohort Study, and sequenced the mouth
rinsing samples for the 16S rRNA gene using Illumina MiSeq.
Results: A total of 693 OTUs were obtained. For each OTU, the
prevalence was positively correlated to the abundance. A core
microbiome composed of five genera was identified and they
together comprised 83.3% of the community. Taxa in the class
Clostridia increased with aging and family Leptotrichiaceae was
more prevalent in Blacks relative to Whites. Phyla
Bacteroidetes and Tenericutes were more abundant among

6. participants with less education. Smokers were depleted for the
phylum Proteobacteria and enriched for the phylum
Actinobacteria relative to non-smokers; however, these two
phyla were recovered after cessation of smoking. Alcohol
drinkers, especially among Blacks with liquor consumption,
were enriched for class Clostridia (phylum Firmicutes). Tooth
loss was associated with decreased abundance of the phylum
Proteobacteria. Phylum Actinobacteria was less common among
diabetes patients than normal weight controls. Among obese
patients, increased abundance was observed for family
Carnobacteriaceae, and decreased abundance was observed for
family Lachnospiraceae, phylum Cyanobacteria and genus
Catonella.
Conclusion: In the present study, a core oral microbiome was
observed in a cohort comprised largely of Blacks and low-
income adults. Multiple taxa showed strong associations with
demographic and lifestyle factors and diabetes and obesity
etiology. A more comprehensive investigation with a large
sample size is warranted.
From the Abstract Answer the following questions .
1. What unknown question is the scientist/author addressing or
attempting to answer and or understand?
2. From reading the abstract, what do you propose the
hypothesis to be?
3. What model was used?
4. Give at least one outcome or result of the study.
5. Name one experimental tool that was used to achieve the
above outcome.
6. What is the importance of the study?

7. 7. Does this study provide any broader impact for mankind?
Why or why not?
8. What protein(s) or cell type is (are) the target(s) of this
study? What other protein or cell type might be used to address
the problem?
Causes and consequences of genetic conflicts
Conflict is pervasive in biology, and even occurs at the genetic
level. For example, host-virus interactions often play out as
conflict between host immune genes and viral genes that
antagonize them. Such genetic conflicts often escalate into
evolutionary ‘arms races’. We study the causes and functional
consequences of genetic conflicts to gain insights into evolution
as well as disease mechanisms.
Host-virus conflict
The ability of host cells to sense viruses is critical to
successfully fight off viral infections. Host protein MAVS plays
a central role in sensing infection by RNA viruses.
Interestingly, many viruses have evolved ways to antagonize
MAVS as a way to evade detection by the host. Our
phylogenetic analyses show that MAVS has recurrently and
rapidly evolved in primates, indicating that it has been engaged
in an arms race with viral antagonists throughout primate
history.
Hepatitis C virus, which infects almost 200 million people
worldwide and causes liver disease, is one such virus that
antagonizes MAVS protein. Interestingly, based on functional

8. experimentation, we found that MAVS from multiple primate
species have independently acquired adaptive amino acid
changes that protect it from HCV antagonism. Remarkably,
most of these changes occur at the same single residue. Thus,
multiple primate species have independently converged on the
same strategy to escape from HCV antagonism.
Conflict between mtDNA and nuclear genome
While it is easy to recognize the conflict of interest between
host and viruses, conflict can also occur between genomes
within the same cell. Mitochondria possess their own genome
(mtDNA), which is exclusively transmitted through females.
This uniparental inheritance renders natural selection
ineffective at removing mtDNA mutations that are deleterious to
males but neutral or beneficial in females. While the male-
harming effects of mutations in mtDNA are of little
consequence for its own evolutionary success, they are clearly
detrimental for the long-term evolutionary survival of the
nuclear genome, which requires males to be healthy. Thus, the
nuclear genome is predicted to encode suppressors that restore
decreases in male fitness caused by mutations in mtDNA. Thus,
analogous to host-virus interaction, a back and forth arms race
is predicted to ensue between mtDNA and the nuclear genome.
We utilized a novel experimental evolution strategy in
Drosophila melanogaster to study male-harming mtDNA
mutations and their suppression by the nuclear genome. We
recovered a single mutation in mtDNA that specifically causes
an age-dependent decrease in male fertility. Cell biological
analyses of sperm development indicate defects in sperm cell
maturation. Interestingly, the nuclear genomes from a number of
wild isolates are able to suppress the male fertility defect
caused by the this mutation. Taken together, our study reveal
the cellular and molecular dynamics and disease consequences
of the evolutionary arms race between mtDNA and the nuclear
genome.

9. From the Abstract Answer the following questions .
1. What unknown question is the scientist/author addressing or
attempting to answer and or understand?
2. From reading the abstract, what do you propose the
hypothesis to be?
3. What model was used?
4. Give at least one outcome or result of the study.
5. Name one experimental tool that was used to achieve the
above outcome.
6. What is the importance of the study?
7. Does this study provide any broader impact for mankind?
Why or why not?
8. What protein(s) or cell type is (are) the target(s) of this
study? What other protein or cell type might be used to address
the problem?
Optimization of DNase Hypersensitivity Site Detection
Protocols for Genome-wide Mapping of Regulatory DNA
Regions
ABSTRACT
Chromatin accessibility is closely associated with fundamental
biological processes such as gene expression, replication, and

10. genome organization. Chromatin regions that are actively
involved in regulation of transcription assume an open
confirmation, thus become hypersensitive to DNase I digestion.
Using the recently developed massive parallel sequencing
approach, it is possible to map DNase I hypersensitive sites
(DHS) genome wide, allowing genome-wide assessment of
regulatory regions. To study DHS in different tissues, cells or
organisms, it is necessary to optimize the protocols for nucleus
isolation, DNase digestion and DNA sequencing.
We are developing and optimizing protocols to be applied in the
analysis of regulatory DNA elements in the chicken. Nuclei are
isolated from liver and spleen tissue, digested with DNase I at
various concentration and time. After digestion, DNA is
isolated and examined by gel electrophoresis. DNase
Hypersensitivity sites are validated by quantitative PCR assay.
Optimized protocols will be applied in genome wide mapping of
regulatory features in chickens.
From the Abstract Answer the following questions .
1. What unknown question is the scientist/author addressing or
attempting to answer and or understand?
2. From reading the abstract, what do you propose the
hypothesis to be?
3. What model was used?
4. Give at least one outcome or result of the study.
5. Name one experimental tool that was used to achieve the
above outcome.
6. What is the importance of the study?
7. Does this study provide any broader impact for mankind?
Why or why not?

11. 8. What protein(s) or cell type is (are) the target(s) of this
study? What other protein or cell type might be used to address
the problem?
Background: The utility of microbial secondary metabolites for
the treatment of human cancers has emerged as alternative
sources of new drugs for the treatment of this disease. To this
end we have investigated the antitumor properties of violacein,
a purple pigment produced by Chromobacterium violaceum in
lung, breast cancer and glioblastoma, the most common and
aggressive type of malignant primary brain tumor. Violacein
was purified from a C. violaceum strain (14N23) isolated from
environmental samples collected in the Tennessee Copper
Basin. Method: Purification and analysis of violacein, Dose
response experiments, Time course analysis, Cell migration
assay and Western blot were performed. Results: Clinically
applicable concentrations of violacein were demonstrated to
inhibit the proliferative capacity of solid tumor cell lines, as a
consequence of promoting apoptotic cell death as indicated by
PARP cleavage and p44/42 MAPK signaling. Collectively, this
provides mechanistic evidence that violacein elicits
Extracellular-signal Regulated Kinase (ERK) induced apoptosis
via the intrinsic pathway. The antimalignant properties of
violacein in this study were further demonstrated by its
inhibitory effects on brain tumor cell migration, specifically
glioblastomas one of the most invasive and therapeutically
resistant neoplasms to treat clinically. Additionally, solid
tumors studied here displayed differential cellular responses and
sensitivities to violacein as observed with morphologically
induced cellular changes that contributed to its antimigratory
properties. Conclusion: In conclusion violacein is a novel
natural product with the potential to kill tumor cells in several
types of human cancers, as well as prevent disease recurrence

12. by antagonizing cellular process that contribute to metastatic
invasion.
From the Abstract Answer the following questions .
1. What unknown question is the scientist/author addressing or
attempting to answer and or understand?
2. From reading the abstract, what do you propose the
hypothesis to be?
3. What model was used?
4. Give at least one outcome or result of the study.
5. Name one experimental tool that was used to achieve the
above outcome.
6. What is the importance of the study?
7. Does this study provide any broader impact for mankind?
Why or why not?
8. What protein(s) or cell type is (are) the target(s) of this
study? What other protein or cell type might be used to address
the problem?