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Tuberkulozes mikrobioloģiskā
        diagnostika
    Mycobacterium tuberculosis
1. Materiāls: krēpas, kuņģa sekrēts, urīns, pleiras
   šķidrums, liquor cerebrospinalis, locītavu
   šķidrums, biopsiju materiāls, asinis.
2. Transportbarotne («Šokolādes», Stjuarta u.c.)
3. Dekontaminācija un koncentrācija- nesterilais
   materiāls (no krēpām u.c.) jāskalo ar N-acetyl-L-
   cysteine un tad ar NaOH, jāneitralizē pēctam ar
   buferi un jācentrifugē.
4. Krāsošana-krēpas, eksudātu pēc Cīla-Nēlsena.
   Urīnu un kuņģa sekrētu nav jēgas, tur
   saprofītiskās       mikobaktērijas      traucēs.
   Fluorescentā mikroskopija ar auramīna-rodamīnu
Auramīna-rodamīna fluorescentais
           krāsojums
kolonijas




Cīla-Nēlsena krāsojums
Acid fast stain demonstrating chording
5. Kultivēšana un identificēšana
• Veeraraghavan's modified medium V (T2) ???
• The rapid radiometric culture system or
  BACTEC (Becton-Dickinson)
• Lēvenšteina-Jensena diferenciāldiagnostiskā.
Latvijas infektoloģijas centra Tuberkulozes un plaušu slimību klīnikas
Mikobakteriologijas laboratorija. Lowenstein–Jensen
Tuberkulozes mikrobioloģiskā diagnostika
Tuberkulozes mikrobioloģiskā diagnostika

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Tuberkulozes mikrobioloģiskā diagnostika

  • 1. Tuberkulozes mikrobioloģiskā diagnostika Mycobacterium tuberculosis
  • 2. 1. Materiāls: krēpas, kuņģa sekrēts, urīns, pleiras šķidrums, liquor cerebrospinalis, locītavu šķidrums, biopsiju materiāls, asinis. 2. Transportbarotne («Šokolādes», Stjuarta u.c.) 3. Dekontaminācija un koncentrācija- nesterilais materiāls (no krēpām u.c.) jāskalo ar N-acetyl-L- cysteine un tad ar NaOH, jāneitralizē pēctam ar buferi un jācentrifugē. 4. Krāsošana-krēpas, eksudātu pēc Cīla-Nēlsena. Urīnu un kuņģa sekrētu nav jēgas, tur saprofītiskās mikobaktērijas traucēs. Fluorescentā mikroskopija ar auramīna-rodamīnu
  • 5. Acid fast stain demonstrating chording
  • 6. 5. Kultivēšana un identificēšana • Veeraraghavan's modified medium V (T2) ??? • The rapid radiometric culture system or BACTEC (Becton-Dickinson) • Lēvenšteina-Jensena diferenciāldiagnostiskā.
  • 7. Latvijas infektoloģijas centra Tuberkulozes un plaušu slimību klīnikas Mikobakteriologijas laboratorija. Lowenstein–Jensen

Editor's Notes

  1. Pozitīvstuberkulīna tests nedod apstiprinājumu aktīvai slimības izpausmei, tikai tuberkulozs ierosinātāja izolēšana piedāvā ticamus rezultātus.
  2. . It is a rich synthetic medium consisting of amino acids, salts and vitamins. It enables the growth of mycobacteria within 48 hrs. The study was carried out to compare the results of Veeraraghavan's liquid medium with LJ medium in routine clinical laboratory. The results of this study indicate that the rapid method gives consistently better results than those obtained by the standard method.