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Līga Vasariņa, MF II, 12. grupa
   Asinis (drudža laikā )
   Uztriepe no eritrēmas
   Var arī audzēt materiālu, kas iegūts no ērcēm

   CSF vai locītavu šķidrums
   Magnēziju saturoša transportbarotne
   Hemoline
   Port-a-cul
   Šķidras barotnes, kas satur asinis, serumu vai
    audus.
   Ātri aug cāļa embrijā
   Visātrāk aug Barbour-Stoenner-Kelly barotnē
    (BSK II) (šķidrā) + rifampīns, fosfomicīns,
    amfotericīns B (lai reducētu kontamināciju ar
    citām baktērijām vai sēnēm)
    Speciālas barotnes (35 C (5-10 d))
   Kustīgas
    ◦ Tumšā redzes lauka mikroskopija
    ◦ Pusšķidrais agars
   Mikroskopēšana
   Seroloģiska IgM/ IgG noteikšana (IgM – 2 ned
        pēc inficēšanās, max – 3-6 ned. Pēc 30 d IgG
        testi ir ticamāki)
       ELISA, EIA
       PCR




Wampole® Borrelia
burgdorferi IgM ELISA II.
Wampole ELISA II LYME
IgM
   A3-
    imunokompetents
   B3, B4 -
    imunosupresēts
   Jutīgas pret doksicilīnu, amoksicilīnu,
    cefrtiaksonu
   Nav nozīmīgas rezistences pret a/b
Boreliozes (2)

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Boreliozes (2)

  • 1. Līga Vasariņa, MF II, 12. grupa
  • 2. Asinis (drudža laikā )  Uztriepe no eritrēmas  Var arī audzēt materiālu, kas iegūts no ērcēm  CSF vai locītavu šķidrums
  • 3. Magnēziju saturoša transportbarotne  Hemoline  Port-a-cul
  • 4. Šķidras barotnes, kas satur asinis, serumu vai audus.  Ātri aug cāļa embrijā  Visātrāk aug Barbour-Stoenner-Kelly barotnē (BSK II) (šķidrā) + rifampīns, fosfomicīns, amfotericīns B (lai reducētu kontamināciju ar citām baktērijām vai sēnēm)  Speciālas barotnes (35 C (5-10 d))
  • 5. Kustīgas ◦ Tumšā redzes lauka mikroskopija ◦ Pusšķidrais agars  Mikroskopēšana
  • 6. Seroloģiska IgM/ IgG noteikšana (IgM – 2 ned pēc inficēšanās, max – 3-6 ned. Pēc 30 d IgG testi ir ticamāki)  ELISA, EIA  PCR Wampole® Borrelia burgdorferi IgM ELISA II. Wampole ELISA II LYME IgM
  • 7. A3- imunokompetents  B3, B4 - imunosupresēts
  • 8. Jutīgas pret doksicilīnu, amoksicilīnu, cefrtiaksonu  Nav nozīmīgas rezistences pret a/b