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Enzyme action
WALT: To be able to describe how enzymes work.
WILF:
~ Recall what the word denatured means. (D)
~ Describe how factors (temperature, pH and
concentration) can affect the activity of enzymes. (C)
~ Explain the model of the ‘Lock and Key’ hypothesis of
showing how enzymes work. (B)
~ Explain how enzymes work referring to the active site
and how it can be affected . (A/A*)
Keywords
enzyme active site Lock and Key hypothesis
substrate
The ‘lock and key’ hypothesis
Substrate
molecules

Active site

Reaction happens (bonds
either made or broken)

Product

Enzymes have a specific 3D
structure

Enzyme

Enzyme-substrate
complex
Breaker Enzymes
Builder Enzymes
The ‘lock and key’ hypothesis
Task: Copy this
image, label and add
notes to explain how
the ‘lock and key’
hypothesis works.
Keywords
enzyme substrate active site
3D shape specific reaction
product
The ‘lock and key’ hypothesis
A change in temperature or pH can affect the bonds that
hold the protein together. This causes the enzyme to
denature, loose its shape.
Factors which affect enzyme
reactions

Use information on
pg 54 to make
notes on how each
factor affects the
rate of reaction.
Enzyme triominoes
1. Cut out all your
triangle pieces.
2. Match up a
question with an
ANSWER.
3. Create 3 of your
own question
and answers.
4. Glue into your
books.

You should end up with a
triangle shape like the one
above.
The ‘lock and key’ hypothesis

The ‘lock and key’ hypothesis

Copy and label the diagram from the board and then use the
keywords to fill in the gaps.

Copy and label the diagram from the board and then use the
keywords to fill in the gaps.

Enzymes have a ________________ 3d shape.

Enzymes have a ________________ 3d shape.

The molecules that enzymes work on are called
____________. This molecule fits into the enzymes
____________ _________, this is a different shape in each
type of enzyme.

The molecules that enzymes work on are called
____________. This molecule fits into the enzymes
____________ _________, this is a different shape in each
type of enzyme.

The reaction takes place, this could involve ________ being
made or broken. After the reaction the ____________ is
released.

The reaction takes place, this could involve ________ being
made or broken. After the reaction the ____________ is
released.

The ‘lock and key’ hypothesis

The ‘lock and key’ hypothesis

Copy and label the diagram from the board and then use the
keywords to fill in the gaps.

Copy and label the diagram from the board and then use the
keywords to fill in the gaps.

Enzymes have a ________________ 3d shape.

Enzymes have a ________________ 3d shape.

The molecules that enzymes work on are called
____________. This molecule fits into the enzymes
____________ _________, this is a different shape in each
type of enzyme.

The molecules that enzymes work on are called
____________. This molecule fits into the enzymes
____________ _________, this is a different shape in each
type of enzyme.

The reaction takes place, this could involve ________ being
made or broken. After the reaction the ____________ is
released.

The reaction takes place, this could involve ________ being
made or broken. After the reaction the ____________ is
released.

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B2.14 enzyme action

  • 1. Enzyme action WALT: To be able to describe how enzymes work. WILF: ~ Recall what the word denatured means. (D) ~ Describe how factors (temperature, pH and concentration) can affect the activity of enzymes. (C) ~ Explain the model of the ‘Lock and Key’ hypothesis of showing how enzymes work. (B) ~ Explain how enzymes work referring to the active site and how it can be affected . (A/A*) Keywords enzyme active site Lock and Key hypothesis substrate
  • 2. The ‘lock and key’ hypothesis Substrate molecules Active site Reaction happens (bonds either made or broken) Product Enzymes have a specific 3D structure Enzyme Enzyme-substrate complex
  • 5. The ‘lock and key’ hypothesis Task: Copy this image, label and add notes to explain how the ‘lock and key’ hypothesis works. Keywords enzyme substrate active site 3D shape specific reaction product
  • 6. The ‘lock and key’ hypothesis A change in temperature or pH can affect the bonds that hold the protein together. This causes the enzyme to denature, loose its shape.
  • 7. Factors which affect enzyme reactions Use information on pg 54 to make notes on how each factor affects the rate of reaction.
  • 8. Enzyme triominoes 1. Cut out all your triangle pieces. 2. Match up a question with an ANSWER. 3. Create 3 of your own question and answers. 4. Glue into your books. You should end up with a triangle shape like the one above.
  • 9. The ‘lock and key’ hypothesis The ‘lock and key’ hypothesis Copy and label the diagram from the board and then use the keywords to fill in the gaps. Copy and label the diagram from the board and then use the keywords to fill in the gaps. Enzymes have a ________________ 3d shape. Enzymes have a ________________ 3d shape. The molecules that enzymes work on are called ____________. This molecule fits into the enzymes ____________ _________, this is a different shape in each type of enzyme. The molecules that enzymes work on are called ____________. This molecule fits into the enzymes ____________ _________, this is a different shape in each type of enzyme. The reaction takes place, this could involve ________ being made or broken. After the reaction the ____________ is released. The reaction takes place, this could involve ________ being made or broken. After the reaction the ____________ is released. The ‘lock and key’ hypothesis The ‘lock and key’ hypothesis Copy and label the diagram from the board and then use the keywords to fill in the gaps. Copy and label the diagram from the board and then use the keywords to fill in the gaps. Enzymes have a ________________ 3d shape. Enzymes have a ________________ 3d shape. The molecules that enzymes work on are called ____________. This molecule fits into the enzymes ____________ _________, this is a different shape in each type of enzyme. The molecules that enzymes work on are called ____________. This molecule fits into the enzymes ____________ _________, this is a different shape in each type of enzyme. The reaction takes place, this could involve ________ being made or broken. After the reaction the ____________ is released. The reaction takes place, this could involve ________ being made or broken. After the reaction the ____________ is released.

Editor's Notes

  1. Recall specific 3d globular shape. Collision theory.
  2. Collision theory.
  3. Recall what is the rate of reaction, ‘how quickly reactants are converted into products’