ELISA troubleshooting guide serves as a checklist for the possible causes and solutions with respect to some of the most commonly encountered problems from the ELISA assays.
2. Troubleshooting Guide
The following guide serves as a checklist for the possible causes and solutions with respect to some of the
most commonly encountered problems from the ELISA assays.
7. Saturated Signal
- Ensure wells are pre-processed to prevent non-specific binding
- Use affinity-purified antibody and preferably one that is pre- adsorbed.
- Use serum (5-10%) from same species as secondary antibody (bovine serum is also
recommended)