7. Fish visual census
By using a fish visual census method (Figure 7) along 100 m length transects with 5 times
replicates parallel to the coast to count (quantify) the abundance and community composition
of fish on a transect. Observers will swim at a constant speed and becareful to not count the
same fish or group of fish twice as they can move away from the diver along the transect.
General procedures:
Conducted during daylight hours
Lay out a 100 m transect tape at depths ;
Wait for 5 to 15 minutes after laying the line before
counting to allow fishes to resume normal behaviour;
Swim slowly along the transect recording fish encountered
in a 5 m belt and 5 m tunnel above the transect; (Figure 8)
Count the actual numbers of fish species seen within the
transect strip
Equipment required:
Tape measures
Underwater slate
SCUBA equipment
Underwater Camera
10. At the seven sites, seawater column profile will be described by taking In situ measurements
for pH, Conductivity (and salinity), Temperature, Dissolved Oxygen, Turbidity from each site
from three depths (0.5 m from surface, mid water depth; and 0.5 m from the seabed).
Parameters will be measured at the site using handheld devices Eureka Manta Multiprobes or
YSI Professional plus Water Quality Instrument.
Seawater sampling will be conducted for water column profile for in situ measurements and
discrete sampling for analyzing all ambient sea water quality parameters.
Water Column Profile
Eureka Manta Multiprobes
YSI Professional Plus
Figure 8: Multiprobes or YSI Professional plus Water Quality Instrument
11. Seawater Quality
Sampling
At seven sites, three seawater sample will be collected from each site. Each water
sample will be analyzed according to EEAA limitations for ambient seawater quality. All
the collected water samples will be sent for accredited analytical laboratory for
analysis.
Seawater samples will be collected from three depths (0.5m from surface, mid water
depth; and 0.5m from the seabed). Each sampling location will be marked by GPS with
the
information of date and time of collection. Seawater sample will be
collected by using Niskin water sampler and transferred to cleaned
water sample containers.
The sample bottles are pre-cleaned with chromic acid, detergent wash, rinsed
with tape water, distilled water and deionised water.
The collected samples will be placed immediately in icebox and filled with
dry ice. The sample bottles will be transported to the laboratory for
processing at the earliest without any delay. If the sample analysis is delayed,
the samples will be stored in a deep freezer at -20º C.
Figure: NISKIN bottles are Non-metallic,
free-flushing water samplers recommended
for general-purpose water sampling.
12. www.themegallery.com
Physical parameters
Vertical profiles of Depth, Temperature, Conductivity / Salinity, Dissolved Oxygen, Turbidity,
Suspended matters, Chlorophyll “a”
Visual observations (litter, oil, slicks any visual disturbance)
Chemical parameters
Eutrophication parameters: water transparency, nutrients: nitrate/nitrite, ammonia, total N and P,
phosphate, silicate and chlorophyll
Hydrocarbons: BTEX, PAH, Total Petroleum Hydrocarbons and Volatile Organic Compounds
Metals (Pb, Cr tot, Cr VI, Cu, Hg, Cd, Zn, Ni, Co, Sn, Ba, Fe)
COD (Chemical Oxygen Demand), BOD5 (Biochemical Oxygen Demand), TOC (Total Organic Carbon).
Biological parameters
Microbiology: Bacteriological parameters (total coliform, Faecal coliform and Faecal streptococci)
Seawater Quality
13. Sediment Quality
Sampling
At six sites, two sediment sample will
be collected from each site. Each
sediment sample will be analyzed
according to EEAA and Canadian
limitations for ambient sediment
quality. All the collected samples will
be sent for accredited analytical
laboratory for analysis.
Figure: The Van Veen Grab sampler use in
sediment sampling in monitoring program
14. Sediment Quality
Physical parameters
In situ description: macroscopic characteristics (grain size, Munsell colour charts, odour, presence of
vegetal frustules, presence of mica or other minerals, presence of shell fragments, presence of organic
matter or contamination)
In situ physical parameters: pH and redox potential (Eh)
Granulometry
Chemical parameters
Eutrophication parameters (nutrients: nitrate/nitrite, ammonia, total N and P, phosphate)
Hydrocarbons: BTEX, PAH, Total Petroleum Hydrocarbons and Volatile Organic Compounds
Metals (Pb, Cr tot, Cr VI, Cu, Hg, Cd, Zn, Ni, Co, Sn, Ba, Fe)
COD (Chemical Oxygen Demand), BOD5 (Biochemical Oxygen Demand), TOC (Total Organic Carbon)
Biological parameters
Microbiology: Bacteriological parameters (total coliform, Faecal coliform and Faecal streptococci) .
15. Phytoplankton: at 6 sites, via specific phytoplankton net, having a 20 µm
mesh and an upper diameter of 40 cm. Samples obtained using vertical haul
method in accordance with UNESCO (Sournia, 1978) and to US-EPA (2002), or
acceptable equivalents.
Zooplankton: at 6 sites, via specific zooplankton net with a 200 µm mesh and
an upper diameter of 57 cm (or equivalent). Sampling to be performed according
to US-EPA (2005) following the vertical tow technique as described in UNESCO
(1979) or acceptable equivalents.
Required instruments: Idromar WP2 type specific phytoplankton net and Idromar
APSTEIN type specific zooplankton net, or acceptable equivalents.
After recovery, the samples are to be transferred into PE containers and fixed by a
solution of seawater and formalin (zooplankton) or lugol (phytoplankton) and stored
and transported safely for laboratory analysis.
Plankton Communities
