Handwritten Text Recognition for manuscripts and early printed texts
Sponsor Day on animal feeding: The role of genetics in Product quality and feed efficiency in swine
1. THE ROLE OF GENETICS IN PRODUCT QUALITY
AND FEED EFFICIENCY IN SWINE
Raquel Quintanilla
IRTA - Animal Breeding and Genetics Program
Torre Marimon, 08140 Caldes de Montbui, Barcelona, Spain.
raquel.quintanilla@irta.cat
IRTA Sponsor Day, Mas de Bover, May 15th-16th 2014
2. • Polyunsaturated FA (PUFA)
• Monounsaturated FA (MUFA)
• Saturated FA (SFA)
• cholesterol content
Sensory quality
Hedonic evaluation
Technological
Quality
Nutritional Quality
Consumers health
Intramuscular fat and meat quality
Intramuscular fat content
and composition
(fatty acids profile)
LIPID
METABOLISM • Water loss
• Rancidity
• Firmless
• Melting
• Tenderness
• Taste
• Flavour
• Juiceness
3. Principal components analysis of dry-cured ham
sensory attributes and fresh meat fat
Pena, Gallardo, Guàrdia, Reixach, Arnau, Amills & Quintanilla. Journal of Animal Science 2013.
350 Duroc barrows
RAW MEAT
Gluteus medius
•Intramuscular fat
•Fatty Acids
•Cholesterol content
ORGANOLEPTIC
CHARACTERISATION
DRY-CURED HAMS:
6 expert panelists
•Appearence
•Taste-flavour
•Texture
2 muscles
•biceps femoris
•semimembranosus
A
B SM
BF
Sección B
Sección A
SM
BF
IMF
CHOL
SFA
PUFA
MUFA
colour
marbling
overall liking
adhesiveness
bitterness
aged
mould
matured
sweetness
hardness
crumbliness
fibrousness
fat melting
metallic
pastiness
piquantness
saltiness
umami
colour
marbling
overall likingadhesiveness
bitterness
aged
mould
matured
sweetness
hardness
crumbliness
fibrousness
fat melting
metallic
pastiness
piquantness
saltiness
umami
-1
-0.5
0
0.5
1
-1 -0.5 0 0.5 1
Component2(18.34%)
Component 1 (19.25%)
gluteus medius biceps femoris semimembranosus
4. GENETICS OF LIPID METABOLISM & MEAT QUALITY
A research line conducted at IRTA since 1996 in different pig
populations (Du and Ib)
GENERAL OBJECTIVE:
Identifying genetic mechanisms underlying lipid
metabolism traits in pigs, with special attention in those
related with muscle fat deposition, and nutritional and
sensorial properties of fresh pork and dry-cured ham,
and contributing new selection strategies.
• Identify genes and polymorphisms (GAS – MAS)
• Disentangle genetic pathways and regulatory
mechanisms
• Implement Genomic Selection schemes
IBMAP projects
1996-2011
IRTA-INIA-UAB
LIPGEN projects
2003-present
IRTA-CRAG
IBERCAL project
2013-present
IRTA - UEX
5. THE ‘OMES AND ‘OMICS PUZZLE
MULTIDISCIPLINARY APPROACHES ARE REQUIRED !!!!
Genome Proteome
mRNA
DNA
Transcriptome
Microbiome
Metabolome
PHENOME
ENVIRONMENT
(NUTRITION)
PIG
GENETICS
THE BASIC MODEL FOR A GENETIST
= Genetics + Environment (Nutrition) + G * E + e
Genetic gain
Generation
N+1 Genetic variability within population
basis for SELECTION
Individual
phenotype
Selection
Epigenetics
Nutrigenomics
6. Recording PHENOME – IRTA EXPERIMENTAL FACILITIES
Controlling
Growing period
IRTA-CAP
- Weight, Fat
- Behavoir
- Individual Feed
intake control
Experimental
Slaughterhouse
Carcass traits
Meat color
Physical-chemical
measures
Meat Analysis
IRTA-CTA
- Intramuscular fat
- Cholesterol in muscle
-Fatty acids profile
- Color
Sensory
characterisation
- Visual, texture and
flavour attributes
(carried out by
expert panelists)
7. INFORMATION used in our researches
350 Duroc individuals: - Genealogy (pedigree)
- more than 100 Phenotypes
Fattening
Control at IRTA-CAP
- Weights
- Backfat
- Serum lipid levels
- Feed control
Slaughter
Carcass and Meat
- Weights
- Conformation
- Fatness
- Physical-chemical
measures of meat
Meat (two muscles)
Analyses at IRTA-CTA
- Color
- Intramuscular fat
- Cholesterol in muscle
- Fatty acids profile
Organoleptic profile
of dry-cured hams
Analyses at IRTA-CTA
- Visual, texture and
flavour attributes
(carried out by expert
panelists)
• DNA - High density genotypes (Illumina Porcine 60K+SNP iSelect Beadchip)
> 60,000 SNP distributed across the porcine genome
• RNA - Expression profiles (GeneChip Porcine Genome Array, Affymetrix)
20,201 GENES (23,937 PROBES)
• Sequence (Next Generation Sequencing; Illumina HiSeq 2000)
• DNA (genome) sequence
• mRNA sequence (RNA-Seq)
PHENOMICSGENOMICS&
TRANSCRIPTOMICS
LIPGEN (350 Du)
IBERCAL (200 Ib)
8. CAN WE IMPROVE IMF BY SELECTION?
HERITABILITIES
Longissimus
dorsi
Gluteus
medius
Intramuscular fat (%) 0.65 **** 0.58 ****
Cholesterol content 0.20 * 0.22 *
Oleic FA (%) 0.15 * 0.21 *
Palmitic FA (%) 0.30 ** 0.30 **
Stearic FA (%) 0.33 *** 0.53 ***
Omega 6 FA (%) 0.14 n.s. 0.14 *
Omega 3 FA (%) 0.14 n.s. 0.16 *
Heritability of
intramuscular fat
content and FA profile
in studied populations
Casellas, Noguera, Reixach, Díaz,
Amills & Quintanilla. Journal of
Animal Science 2010.
Heritability of
intramuscular fat
Knapp et al. 1997
0.38 (LW) 0.67 (LD) 0.32 (Pi)
Larzul et al. 1997
0.44 (LW)
Hermesch et al. 2000
0.35 (LW & LD)
Fernández et al. 2003
0.25 (IB)
Suzuki et al. 2005
0.39 (Du)
YES, we could implement a selection scheme on IMF and expect a
response to selection (moderate to high heritabilities)
BUT Phenotyping (measurements) expensive / difficult
EBV for candidates based on relatives records (lower accuracy)
Correlated response in BFT
Genetic correlation with fatness (BFT): 0.25-0.40
Gene Assisted Selection (GAS)
Genomic Selection (GS)
Genetic gain
Generation
N+1
Selection
9. Varona et al. Genetical Research 2002
Ovilo et al. J. Anim. Sci. 2002
Perez-Enciso et al. Genetics. 2002
Clop et al. Mamm. Genome. 2003
Peréz-Enciso et al. J. Anim. Sci. 2005
Mercadé et al. Mamm. Genome 2005
Gallardo et al. Physiol. Genom. 2008
Quintanilla et al. J. Anim. Sci. 2011
Gallardo et al. Anim. Genet. 2011
Pena et al. J. Anim. Sci. 2013
GENOMIC REGIONS ASSOCIATED TO PHENOTYPES
QTL detected with low density panels
0
2
4
6
8
10
12
SSC6
IMF QTL
Q – Iberian allele
q – Landrace allele
IBERIAN (QQ)
Q allele high IMF
GWAS (Genome-Wide Association Studies)
using high density >60K SNP panels
Possibility of a reciprocal introgression of the Iberian allele in other breeds
Hernández, Amills, Pena, Mercadé, Manunza & Quintanilla. J. Anim. Sci. 2013
Backfat thickness and Intramuscular fat: distribution of
additive genetic variance across genome
GENOMICS
10. GENE EXPRESSION
• Differential gene expression in muscle
Muscle transcriptomic profiles in pigs with
divergent phenotypes for fatness.
Cánovas , Quintanilla, Amills, Pena. BMC Genomics 2010
• eQTL mapping (genetical genomics)
11 trans-regulatory hotspots on chromosomes 1, 2, 3, 5, 6, 7, 12
Cánovas, Pena, Gallardo, Ramírez, Amills & Quintanilla. PLOS ONE 2011
0
1
2
3
4
5
6
Fold-changeratio
Microarray ratio
qPCR ratio
TRANSCRIPTOMICS
11. ACACA Fatty acids profile
Gallardo et al. 2009. Anim. Genet.
LDLR, APOB Serum lipids
Pena et al. 2009. Anim. Biotech.
HDLBP Intramuscular fat
Cánovas et al. 2009. Livest. Sci.
HMGCR Cholesterol & Fatty acids
Cánovas et al. 2010. Animal.
FATP1, FATP2 Fatty acids profile.
Melo et al. Livest. Sci. 2012
LRP12, TRIB1, APOD Serum lipids &
Fatty acids profile.
Melo et al. J Anim Sci. 2012
SCD Fatty acids profile.
Aznárez et al. 2012.
Amplification &
Sequencing
Identification segregating
polymorphisms
Genotyping animal
population
DNA from a sample
of pigs
ASSOCIATION STUDIES
CANDIDATE GENE
POSITIONAL & FUNCTIONAL CANDIDATE GENESGENOMICS &
TRANSCRIPTOMICS
13. Use of genomic information in a selection scheme
Genomic Selection: using massive genotypes (60K SNPs)
information in genetic evaluation (GEBV)
Current studies regarding implementation of GS
models and methods (genomic BLUP, Bayes A, B and C, Lasso)
increase of selection accuracy in several scenarios
integration of crossbreed information in purebreed selection
reduced SNP panels – Reduced cost
Genomic selection of purebreeds for crossbred performance. Genetic Selection Evolution 2009.
Modifying growth curve parameters by multitrait genomic selection. Journal of Animal Science 2011.
Expected advantages
• Increase of accuracy
• Avoid phenotyping of candidates
• Reduce generation interval
Questions
• Models and methods
• Reference population size
• LD must be reevaluated. Frequency?
• Long-term consequences
• Cost - Revenue
14. • Nutrigenomics
Experiment in progress: effect of different FA diet in
gene expression and muscle fat deposition
• Gene networks
• Epistatic & co-expression networks
• Gene networks across tissues
• Key genes modulating topology
• Analysis of DNA & RNA sequence:
• New transcriptomic & genomic variants
• Metabolome and Microbiome information
IN PROGRESS & FUTURE STUDIES
towards more holistic and multidisciplinary approaches
CHALLENGE: Integral analyses of all available information
15. Investigating possibilities and benefits of different methods
to improve feed efficiency in sire lines of pigs and rabbits
Alternative selection criteria (RFI, group measures, regression)
NUTRIGENOMICS experiment:
Interaction genotype*feeding regime
Genetics of feeding behavioral traits
Breeding value estimation models:
• Including social interactions and/or individual
sensitivity (robustness) to environmental factors such
as sub-clinical diseases
• Including genomic information (GS)
IN PROGRESS & FUTURE STUDIES
Feed efficiency
Improving efficiency of sows taking into account the relationships
between the use of food resources, mobilization of body reserves,
robustness, reproductive function, litter homogeneity and survival.
FEED
EFFICIENCY
16. Collaborators
Armand Sánchez
Marcel Amills
Miguel Pérez-Enciso
Josep M. Folch
Quim Casellas
Angela Cánovas
David Gallardo
Luis Silió
M. Carmen Rodríguez
Cristina Óvilo
Ana Fernández
Estefania Alvés
Romi Pena
Joan EstanyLuis Varona
ANIMAL BREEDING &
GENETICS
Joan Tibau
José L. Noguera
Noelia Ibáñez
Juan P. Sánchez
Miriam Piles
Quim Soler
Mateu Tulsà
PRODUCT QUALITY
M.Angels Oliver
Marina Gispert
FOOD TECHNOLOGY
Dolors Guàrdia
Jacint Arnau
FUNCTIONALITY &
NUTRITION
Isabel Díaz
Josep Reixach
Emilio Magallón
NUTRITION &
WELFARE
J. Brufau
Torrallardona
E. Esteve
R. Lizardo
E. Fàbrega
A. Dalmau
A. Velarde
GIRO
F. Prenafeta
M. Vinyes
A. Bonmatí