Notch signaling is a highly conserved and essential cellular communication pathway that plays a crucial role in various developmental processes and tissue homeostasis. Here's a brief description suitable for a SlideShare presentation: **Slide 1: Title** *Notch Signaling: Unlocking Cellular Communication* **Slide 2: Introduction** - Notch signaling is a fundamental pathway regulating cell-to-cell communication. - It influences diverse processes from embryogenesis to adult tissue maintenance. **Slide 3: Key Components** - Notch receptors: Proteins on cell membranes. - Ligands: Proteins binding to receptors. - Intracellular domain: The active signaling component. **Slide 4: How It Works** - Notch receptors interact with ligands on neighboring cells. - This interaction triggers cleavage and release of the Notch intracellular domain. - Intracellular domain translocates to the nucleus. **Slide 5: Role in Development** - Controls cell fate decisions during embryonic development. - Ensures proper tissue differentiation and patterning. **Slide 6: Adult Tissue Homeostasis** - Maintains tissues by regulating cell proliferation and differentiation. - Essential for processes like stem cell maintenance. **Slide 7: Notch in Diseases** - Dysregulation linked to cancer, cardiovascular diseases, and developmental disorders. - Potential therapeutic target. **Slide 8: Notch Signaling in Research** - Continuously studied for its diverse roles. - Target for drug development and regenerative medicine. **Slide 9: Conclusion** - Notch signaling is a pivotal pathway in cellular communication. - Understanding its intricacies can lead to novel treatments and insights into development. **Slide 10: Questions** - Open the floor for questions and discussion. This concise presentation provides a basic overview of Notch signaling, its components, functions, and relevance in both development and disease. You can expand on each slide with more detailed information as needed.

1. NOTCH SIGNALLING

2. INTRODUCTION
• Notch is a single transmembrane protein located on the
cell surface with transmembrane ligands.
• The signalling event is triggered by cell-cell contact
which is essential for this event.
• Notch plays a dual role as a transmembrane receptor
and a transcription factor.
• The notch ligands are also single-pass transmembrane
proteins and are members of the DSL
(Delta/Serrate/LAG-2) family of proteins.

3. Domains of notch protein
• Notch protein has 3 domains
1. Extracellular domain
2. Transmembrane domain
3. Intracellular domain
• The notch extracellular domain is composed
primarily of small cystine-rich motifs
called EGF-like repeats.

9. Signal sending
cell
Signal receiving
cell
MAML=Master like mind

10. NOTCH signaling pathway
1. In the golgi apparatus the Notch receptor
undergoes proteolytic processing, which is S1
cleavage mediated by Furin proteases.
2. The receptor is transported to the cell surface
membrane.
3. The extracellular domain of the Notch
receptor (Notch-ECD) in the signalling cell
binds with the Notch ligands (Delta, Jagged,
Serrate) expressed by the adjacent cell.

11. NOTCH signaling pathway
4. This induces the second proteolytic step, S2 cleavage
by ADAM family metalloproteases ADAM10. This
releases the extracellular portion of notch (NECD), which
continues to interact with the ligand. The ligand plus the
notch extracellular domain is then endocytosed by the
ligand-expressing cell.
5. This is followed by the release of the Notch
intracellular domain (Notch-ICD) with a tethered
membrane. This is now a substrate for the gamma
secretase enzyme complex, which cleaves the Notch-ICD
from the membrane by S3.

12. NOTCH signaling pathway
6. The resulting active Notch-ICD translocates to
the nucleus and interacts with the CSL protein.
7. In the absence of Notch-ICD, the CSL is
bound to co-repressor. Binding of Notch-ICD
with CSL results in the formation of an active
complex with MAML and other co-activators
and leads to the transcription of Notch targets.

13. Lateral inhibition
• The receptor is normally triggered via direct cell-to-cell
contact, in which the transmembrane proteins of the cells in
direct contact form the ligands that bind the notch receptor.
• The Notch binding allows groups of cells to organize
themselves such that, if one cell expresses a given trait, this
may be switched off in neighbouring cells by the
intercellular notch signal. In this way, groups of cells
influence one another to make large structures.
• Thus, lateral inhibition mechanisms are key to Notch
signaling.