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BIOTECHNOLOGY
MCQs
FREQUENTLY ASKED MCQs
VIDEO
1
BIOTECHNOLOGY
MCQs
FREQUENTLY ASKED MCQs
VIDEO
1
1. Restriction enzymes were discovered by:
a) Smith and Nathans
b) Alexander Fleming
c) Berg
d) None
a) Smith and Nathans
2. Bacteria protect themselves from viruses by
fragmenting viral DNA with
a) Ligase
b) Endonuclease
c) Exonuclease
d) Gyrase
b) Endonuclease
3. Klenow fragment is derived from
a) DNA Ligase
b) DNA Pol-I
c) DNA Pol-II
d) Reverse Transcriptase
b) DNA Pol-I
4. Southern blotting is
a) Attachment of probes to DNA fragments
b) Transfer of DNA fragments from
electrophoretic gel to a nitrocellulose sheet
c) Comparison of DNA fragments to two
sources
d) Transfer of DNA fragments to
electrophoretic gel from cellulose membrane
b) Transfer of DNA fragments from
electrophoretic gel to a nitrocellulose sheet
5. A plasmid is;
a) Used as a DNA vector
b) A type of bacteriophage
c) A type of cDNA
d) A retrovirus
a) Used as a DNA vector
6. The PCR technique uses;
a) Heat resistant DNA polymerase
b) Reverse transcriptase
c) DNA ligase
d) Restriction enzyme
1. Heat resistant DNA
polymerase
7. Which technique rapidly replicated specific
DNA fragments without cloning;
a) Gel elctropherisis
b) cDNA libraries
c) Genetic probe
d) PCR
d) PCR
8. Meristem is;
a) Pathogen free
b) Bacteria free
c) Fungi free
d) Virus free
Virus free
9. Which bacterium is used in the production
of insulin by genetic engineering?
a) Saccharomyces
b) Rhizobium
c) Escherichia
d) Mycobacterium
c) Escherichia
10. The expression of a transgene in the target
tissue is identified by a
a) Transgene
b) Promoter
c) Enhancer
d) Reporter
d) Reporter
11. ———– is used as a vector for cloning into
higher organisms
a) Retrovirus
b) Baculovirus
c) Salmonella typhimurium
d) Rhizopus nigricans
a) Retrovirus
12. Excision and insertion of a gene is called
a) Biotechnology
b) Genetic engineering
c) Cytogenetics
d) Gene therapy
b) Genetic engineering
13. RNA interference helps in
a) Cell proliferation
b) Micropropagation
c) Cell defence
d) Cell differentiation
c) Cell defence
14. The first transgenic plant to be produced is
a) Brinjal
b) Tobacco
c) Rice
d) Cotton
b) Tobacco
15. Which is a genetically modified crop?
a) Bt-cotton
b) Bt-brinjal
c) Golden rice
d) All
d) all

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  • 3. 1. Restriction enzymes were discovered by: a) Smith and Nathans b) Alexander Fleming c) Berg d) None a) Smith and Nathans
  • 4. 2. Bacteria protect themselves from viruses by fragmenting viral DNA with a) Ligase b) Endonuclease c) Exonuclease d) Gyrase b) Endonuclease
  • 5. 3. Klenow fragment is derived from a) DNA Ligase b) DNA Pol-I c) DNA Pol-II d) Reverse Transcriptase b) DNA Pol-I
  • 6. 4. Southern blotting is a) Attachment of probes to DNA fragments b) Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet c) Comparison of DNA fragments to two sources d) Transfer of DNA fragments to electrophoretic gel from cellulose membrane b) Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
  • 7. 5. A plasmid is; a) Used as a DNA vector b) A type of bacteriophage c) A type of cDNA d) A retrovirus a) Used as a DNA vector
  • 8. 6. The PCR technique uses; a) Heat resistant DNA polymerase b) Reverse transcriptase c) DNA ligase d) Restriction enzyme 1. Heat resistant DNA polymerase
  • 9. 7. Which technique rapidly replicated specific DNA fragments without cloning; a) Gel elctropherisis b) cDNA libraries c) Genetic probe d) PCR d) PCR
  • 10. 8. Meristem is; a) Pathogen free b) Bacteria free c) Fungi free d) Virus free Virus free
  • 11. 9. Which bacterium is used in the production of insulin by genetic engineering? a) Saccharomyces b) Rhizobium c) Escherichia d) Mycobacterium c) Escherichia
  • 12. 10. The expression of a transgene in the target tissue is identified by a a) Transgene b) Promoter c) Enhancer d) Reporter d) Reporter
  • 13. 11. ———– is used as a vector for cloning into higher organisms a) Retrovirus b) Baculovirus c) Salmonella typhimurium d) Rhizopus nigricans a) Retrovirus
  • 14. 12. Excision and insertion of a gene is called a) Biotechnology b) Genetic engineering c) Cytogenetics d) Gene therapy b) Genetic engineering
  • 15. 13. RNA interference helps in a) Cell proliferation b) Micropropagation c) Cell defence d) Cell differentiation c) Cell defence
  • 16. 14. The first transgenic plant to be produced is a) Brinjal b) Tobacco c) Rice d) Cotton b) Tobacco
  • 17. 15. Which is a genetically modified crop? a) Bt-cotton b) Bt-brinjal c) Golden rice d) All d) all