1. COLISWEEPER
The world’s first bacterial minesweeper game
ETH Zurich 2013

2. From Minesweeper* to Colisweeper
cm
1.5
*Démineur
!2

3. Analogous Game States
F
P
e
1m
2
a er
G v
O
1
!3
2
Flagging
Playing
Solution

4. Implementation: Biological Circuit
Mine Cell / Sender
chromosomal
AHL
Pconst
Pconst
Non Mine Cell / Receiver
luxI
phoA
Pconst
PLuxR (high sensitivity)
gusA
luxR
nagZ
A
H
L
!4
PLuxR (low sensitivity)
aes

5. Results
Gradient
Signal Processing
Output
Aes GusA NagZ
Magenta Butyrate
Salmon Glucoronide
ACCTGTAGGATCGTACAGGT
ACNNNTAGGATCGTANNNGT
X-GluNac
R2 > 0.95
1
PhoA
1 PhoA+GusA
!5
2
2 PhoA+GusA+Aes
PhoA+NagZ

6. Proof of Principle
AHL
PLac
luxR
Pconst
PLuxR (high)
gfp
PLuxR (low)
luxI
rfp
GFP Expression
A
A: Low - 1 mine nearby
GFP
1
ü
D: None - 0 mines nearby
2
ü
Mine producing AHL
3
ü
B: Medium - 2 mines nearby
C
D
#
B
C: High - 3 mines nearby
RFP [au]
Time: 11 h
!6
RFP
ü

7. Colisweeper - Tool for Education
PLac
Synthetic
Biology I
!
Lab Course
luxR
PLuxR (high)
gfp
PLuxR (low)
rfp
!7

8. Acknowledgments
Prof. Sven Panke
Markus Jeschek
Prof. Yaakov Benenson
Gaspar Morgado
Prof. Jörg Stelling
Sabine Österle
!
Laura Prochazka
!
Benjamin Häfliger
!
Bartolomeo Angelici
!
Lukas Widmer
!
!
!
!
!
!
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!8
Dr. Johannes Härle
Andreas Bosshart
Anke Gehringer
Niels Bürckert
Verena Jäggin
Thomas Horn
Urs Senn

9. Sponsors
!9

10. Merci pour votre attention!
Questions?
!
iGAM4ER 2013
!10

11. Proof of principle - GFP
!11