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GENERAL EDUCATION COMMON ASSIGNMENT
I. TITLE & NUMBER OF THE COURSE: GOVT 2305 or 2306
II. TITLE OF THE ASSIGNMENT: Course Level Assessment -
Content Learning & General Education Outcomes - Government
2305 & 2306
III. GENERAL EDUCATION CORE OBJECTIVES TO BE
ASSESSED WITH THIS:
A. Communication Skills
B. Critical Thinking
C. Social Responsibility
D. Personal Responsibility
IV. DESCRIPTION OF ASSIGNMENT
Objectives of the Writing Assignment
· Learn about a current political issue or problem of political
consequence.
· Develop your Internet and library research skills.
· Evaluate your topic from the perspective of different cultures
and/or political viewpoints as well as your own.
· Improve personal and social responsibility.
· Improve communication and critical thinking skills.
Paper Criteria
· Topic selection— A current governmental or political (not
historical) issue or problem of importance
· Content— address the various sides of the issue with the
student drawing personal conclusions based on their own
analysis of the issue, and demonstrating an understanding of its
complexities. Compare and contrast the different viewpoints of
sources used on your topic taking into account the limits of a
position. Present an informed evaluation of the evidence and
different viewpoint surrounding the topic.
· Paper length - your paper should be at minimum (no
maximum) of 750 words long (not counting headings). The
paper should be typewritten and double-spaced, and based on
information from a minimum of three sources (no maximum).
· Writing style—with few or no typographical errors, misspelled
words, and grammatical errors. Use proper paragraphing and
improve your writing style by revising the paper as many times
as necessary.
· Citations—anytime you borrow someone’s ideas, paraphrase
or quote them, cite all sources using an appropriate method
approved by the instructor. Analyze and question your sources
assumptions.
· A bibliography or “work cited” page also using an appropriate
method approved by the instructor.
Environmental Science Table of Contents
37
Lab 3
Biodiversity
Biodiversity
Concepts to Explore
• Biodiversity
• Species diversity
• Ecosystem diversity
• Genetic diversity
• Natural selection
• Extinction
Introduction
Biodiversity, short for biological diversity, includes the genetic
variation between all organisms, species, and
populations, and all of their complex communities and
ecosystems. It also reflects to the interrelatedness of
genes, species, and ecosystems and their interactions with the
environment. Biodiversity is not evenly distrib-
uted across the globe; rather, it varies greatly and even varies
within regions. It is partially ruled by climate,
whereas tropical regions can support more species than a polar
climate. In whole, biodiversity represents
variation within three levels:
• Species diversity
• Ecosystem diversity
• Genetic diversity
It should be noted that diversity at one of these levels may
not correspond with diversity within other levels. The degree
of biodiversity, and thus the health of an ecosystem, is im-
pacted when any part of that ecosystem becomes endan-
gered or extinct.
The term species refers to a group of similar organisms that
reproduce among themselves. Species diversity refers to
the variation within and between populations of species, as
well as between different species. Sexual reproduction criti-
cally contributes to the variation within species. For exam-
ple, a pea plant that is cross-fertilized with another pea plant
can produce offspring with four different looks! This genetic
mixing creates the diversity seen today.
Figure 1: There are more than 32,000 species of
fish – more than any other vertebrate!
39
Biodiversity
Ecosystem diversity examines the different habitats, biological
communities, and ecological processes in
the biosphere, as well as variation within an individual
ecosystem. The differences in rainforests and deserts
represent the variation between ecosystems. The physical
characteristics that determine ecosystem diversity
are complex, and include biotic and abiotic factors.
? Did You Know...
A present day example of natural
selection can be seen in the cray-
fish population. The British crayfish
are crustaceans that live in rivers in
England. The American crayfish
was introduced to the same bodies
of water that were already populat-
ed by the British crayfish. The
American crayfish are larger, more
aggressive and carry an infection
that kills British crayfish but to
which they are immune. As a re-
sult, the British crayfish are de-
creasing in number and are ex-
pected to become extinct in Britain
within the next 50 years. Thus, the
American crayfish have a genetic
variation that gives them an ad-
vantage over the British crayfish to
survive and reproduce.
The variation of genes within individual organisms is genetic
diversity.
This can be measured within a species as well as between
species. It
plays an important role in survival and adaptability of
organisms to
changing environments.
Diversity is also influenced by natural selection, the key
mechanism of
evolution. The process of natural selection describes
competition be-
tween individual species for resources such as food and space
(habitat). Genetic variations among species provide an
advantage over
other species if those variations result in an ability to survive
and repro-
duce more effectively.
Evidence that supports the theory of natural selection include
the fossil
record of change in earlier species, the chemical and anatomical
simi-
larities of related life forms, the geographical distribution of
related spe-
cies, and the recorded genetic changes in living organisms over
many
generations. Take for example, homologous structures among
different
species, such as the wing of a bird and the forearm of a human.
These
structures provide evidence that embryologically similar
structures can
give rise to different functions based on the needs of the
organism.
Note that natural selection does not try to explain the origin of
life but
rather the later evolution of organisms over time.
Biodiversity is important to the process of evolution because it
provides
the framework on top of which natural selection can occur. As
dis-
cussed above, natural selection determines the genetic fitness,
an or-
ganism's genetic contribution to the next generation, of an
organism.
Natural selection occurs by selecting one trait as "more
advantageous" in a certain environment than anoth-
er. The root of this selection is biodiversity.
Species extinction is not new; species have been evolving and
dying out since life began. Now, however,
species extinction is occurring at an alarming rate, almost
entirely as a direct result of human activities. Sci-
40
Biodiversity
entists recognize five major mass extinctions in the Earth’s
history. The extinctions are measured in terms of
large groups of related species, called families. The five mass
extinction episodes occurred because of major
changes in the prevailing ecological conditions brought about
by climate change, cataclysmic volcanic erup-
tions, or collisions with giant meteors. The sixth mass
extinction appears to be in progress now, and the pri-
mary cause is environmental change brought about by human
activities. Some examples of species on the
“endangered” list are the ivory billed woodpecker, amur
leopard, javan rhinoceros, northern great whale,
mountain gorilla, and the leatherback sea turtle.
Figure 2: The amur leopard is at risk of extinction.
Loss of an individual species can have various effects on the
remaining species in an ecosystem. These ef-
fects depend upon the how important the species is in the
ecosystem. Individual species and ecosystems
have evolved over millions of years into a complex
interdependence. If you remove enough of the key spe-
cies on which the framework is based, then the whole ecosystem
may be in danger of collapsing. Regardless
of a species’ place in the ecosystem, it is important for humans
to take care of the world around us. As peo-
ple become more aware of how their actions impact all living
things they can make adjustments in an effort to
preserve life on all levels.
41
Biodiversity
There are many activities that humans take part in that impact
the environment and biodiversity. The exhaust
from automobile and aircraft travel as well as smoke stacks
from industrial plants are the leading causes of air
pollution, which can have harmful effects on natural resources
and organisms. Two other important factors
which can have an effect on biodiversity are overpopulation and
affluence. Overpopulation means that there
are more people than resources to meet their needs. As people
become more affluent there is an increase in
per capita resource utilization. All of these factors contribute to
overharvesting, habitat degradation, and in-
creased pollution which threaten biodiversity.
42
Biodiversity
Demonstration 1: Interdependence of Species
In this lab, you will use the information provided above to
demonstrate how the presence or absence of one
species can affect the others in an ecosystem. Follow the
procedure below to complete Demonstration 1 on
the interdependence of species.
Materials
5 different colored beads:
White bead represents lichen
Orange bead represents trees
Red bead represents flowers
Yellow bead represents bees
Blue bead represents humans
Lichens
Lichens play a part in the creation of soils from which plants
can obtain nutrients. Like all living
organisms, lichens need nutrients and energy to grow. Nutrients
may be obtained from the air
(including dust), water, and from the substrate organisms grows
on. They obtain energy through
photosynthesis, which is the role of the algal partner. They may
also be incidentally fertilized by
bird and insect dung.
Trees
Most trees, flowers and plants depend on soil for food
(nutrients). Fruiting trees depend on bees
as one means of pollination.
Flowers
Forest flowers and plants depend on trees for shade and wind
protection as well as soils for nutri-
ents.
Bees
Bees depend on flowering plants and trees for food.
Humans
Humans depend upon bees for honey and more importantly for
fruit from trees they pollinate.
43
Biodiversity
Procedure
1. Download the Week 3 Lab Reporting Form from the course
instructions. As you conduct the demonstra-
tion and the experiment, record any hypotheses, observations,
and data on that form.
2. Place all of the beads in a bag.
3. Randomly choose 4 beads from the bag.
4. Identify each bead by the color code in the materials box.
5. Record which species is missing in Table 1 on the Week 3
Lab Reporting Form.
6. Repeat this process 3 more times (or until 3 different beads
are taken out of the diagram). Be sure to rec-
ord which species is missing from each round in Table 1 and
answer the Post-Lab questions on the Week
3 Lab Reporting Form.
Experiment 1: Diversity of Plants
Variations in growing conditions, climate, and numerous other
factors can alter the biodiversity of an ecosys-
tem. As such, biodiversity is often utilized as an indicator of
ecosystem health. In this experiment, you will
grow a sample of seeds under two different conditions. Follow
the procedure below to complete Experiment 1
on the diversity of plants.
Materials
Seed mixture (zinnia, marigold, morning glory,
cosmos, and ryegrass)
Potting soil
(2) 5.5 x 3.5 in Peat pots
10 mL Graduated cylinder
*Water
*You must provide
Procedure
1. Read through the Experiment 1 procedure and then record
your hypothesis on the biodiversity of seeds
grown under two separate conditions on the Week 3 Lab
Reporting Form.
2. Fill your pots loosely with soil until the soil is approximately
1 inch from the top.
3. Pour approximately 40 mL of tap water into your pots (less if
the soil becomes very wet).
44
Biodiversity
4. Lightly scatter your seeds on top of the soil in each
container. This should be a random assignment of
seeds to each pot.
5. Press each seed down about ½ inch into the soil.
6. Place one pot in a sunny, indoor location (on a window sill
that receives sunlight) and the second pot in a
shaded, indoor location (away from all windows, this pot should
not be placed in the dark, just away from
direct sunlight).
7. Observe and water your seeds every day until you see them
grow. These seeds will germinate quickly (3
- 7 days).
8. Complete Table 2 on the Week 3 Lab Reporting Form after
approximately 1 - 2 weeks (or when you see
a reasonable amount of plant growth in the peat pot) and answer
all Post-Lab Questions on the Week 3
Lab Reporting Form. Table 3 (provided on the following page)
provides pictures of the germinated seeds
to help you determine when you should begin entering data, and
what each plant looks like.
45
Weather and Climate Change
Table 3: Picture and Description of Seedlings Grown from Seed
Mixture
Species Observed Picture Description
Zinnia
Short stems with dark green,
rounded leaves
Marigold
Stems are shorter than cosmos
with long skinny leaves (but wid-
er than the cosmos leaves) with
rounded tips
Morning Glory
Tall stems with elephant ear
shaped leaves
Cosmos
Tall stems with long, pointed
leaflets; a lighter green leaf com-
pared to the marigold
Ryegrass
Long, skinny strands of green
grass
46
Appendix
Good Lab Techniques
Good Lab Techniques
Good Laboratory Techniques
Science labs, whether at universities or in your home, are places
of adventure and discovery. One of the first
things scientists learn is how exciting experiments can be.
However, they must also realize science can be
dangerous without some instruction on good laboratory
practices.
• Read the protocol thoroughly before starting any new
experiment.
You should be familiar with the action required every step of
the
way.
• Keep all work spaces free from clutter and dirty dishes.
• Read the labels on all chemicals, and note the chemical safety
rating
on each container. Read all Material Safety Data Sheets
(provided
on www.eScienceLabs.com).
• Thoroughly rinse lab ware (test tubes, beakers, etc.) between
experi-
ments. To do so, wash with a soap and hot water solution using
a
bottle brush to scrub. Rinse completely at least four times. Let
air
dry
• Use a new pipet for each chemical dispensed.
• Wipe up any chemical spills immediately. Check MSDSs for
special
handling instructions (provided on www.eScienceLabs.com).
• Use test tube caps or stoppers to cover test tubes when shaking
or
mixing – not your finger!
A B C
Figure 1: A underpad will
prevent any spilled liquids
from contaminating the sur-
face you work on.
Figure 2: Special measuring tools in make experimentation
easier and more accu- rate in
the lab. A shows a beaker, B graduated cylinders, and C test
tubes in a test tube rack.
67
Good Lab Techniques
• When preparing a solution, refer to a protocol for any specific
instructions on preparation. Weigh out the desired amount of
chemicals, and transfer to a beaker or graduated cylinder.
Add LESS than the required amount of water. Swirl or stir to
dissolve the chemical (you can also pour the solution back
and forth between two test tubes), and once dissolved, trans-
fer to a graduated cylinder and add the required amount of
liquid to achieve the final volume.
• A molar solution is one in which one liter (1L) of solution
con-
tains the number of grams equal to its molecular weight.
For example:
1M = 110 g CaCl x 110 g CaCl/mol CaCl
(The formula weight of CaCl is 110 g/mol)
Figure 3: Disposable pipettes aid in ac-
curate measuring of small volumes of
liquids. It is important to use a new pi-
pette for each chemical to avoid con-
tamination.
• A percent solution can be prepared by percentage of weight of
chemical to 100ml of solvent (w/v) , or
volume of chemical in 100ml of solvent (v/v).
For example:
20 g NaCl + 80 mL H2O = 20% w/v NaCl solution
• Concentrated solutions, such as 10X, or ten times the normal
strength, are diluted such that the final
concentration of the solution is 1X.
For example:
To make a 100 mL solution of 1X TBE from a 10X solution:
10 mL 10X TBE + 90 mL water = 100ml 1X TBE
• Always read the MSDS before disposing of a chemical to
insure it does not require extra measures.
(provided on www.eScienceLabs.com)
• Avoid prolonged exposure of chemicals to direct sunlight and
extreme temperatures. Immediately se-
cure the lid of a chemical after use.
• Prepare a dilution using the following equation:
c1v1 = c2v2
Where c1 is the concentration of the original solution, v1 is the
volume of the original solution, and
c2 and v2 are the corresponding concentration and volume of
the final solution. Since you know c1,
68
Good Lab Techniques
c2, and v2, you solve for v1 to figure out how much of the
original solution is needed to make a cer-
tain volume of a diluted concentration.
• If you are ever required to smell a chemical, always waft a gas
toward you, as shown in the figure
below.. This means to wave your hand over the chemical
towards you. Never directly smell a
chemical. Never smell a gas that is toxic or otherwise
dangerous.
• Use only the chemicals needed for the activity.
• Keep lids closed when a chemical is not being used.
• When diluting an acid, always slowly pour the acid into the
water. Never pour water into an acid,
as this could cause both splashing and/or an explosion.
• Never return excess chemical back to the original bottle. This
can contaminate the chemical sup-
ply.
• Be careful not to interchange lids between different chemical
bottles.
• When pouring a chemical, always hold the lid of the chemical
bottle between your fingers. Never
lay the lid down on a surface. This can contaminate the
chemical supply.
• When using knives or blades, always cut away from yourself.
69
© 2012 eScience Labs, LLC - All rights reserved
68
Lab 3Concepts to ExploreIntroductionDemonstration 1:
Interdependence of
SpeciesLichensMaterialsProcedureAppendixA B C© 2012
eScience Labs, LLC - All rights reserved
Lab 3 – Biodiversity
Demonstration 1: Interdependence of Species
Table 1: Interdependence of Species Results
Round
Species Missing (Bead Color and Name)
1
2
3
4
POST LAB QUESTIONS
1. Explain how the ecosystem was affected by the missing
species for each round of the demonstration.
a. Round 1 =
b. Round 2 =
c. Round 3 =
d. Round 4 =
2. Provide one action we as humans engage in that leads to the
extinction of each of these components.
Action affecting Lichens =
Action affecting Trees =
Action affecting Flowers =
Action affecting Bees =
3. Provide three specific actions that humans can take to
minimize our impact on the ecosystem and ensure the survival
of lichens, trees, flowers, and bees.
Action 1 =
Action 2 =
Action 3 =
Experiment 1: Diversity of Plants
Table 2: Number of Each Plant Species Present in Pot 1 and Pot
2
Species Observed
Number in Pot 1(sunlight)
Number in Pot 2(shade)
Zinnia
Marigold
Morning Glory
Cosmos
Ryegrass
Total Number of Species in Pot:
POST LAB QUESTIONS
1. Develop a hypothesis on which pot you believe will contain
the highest biodiversity.
Hypothesis =
2. Based on the results of your experiment, would you reject or
accept the hypothesis that you produced in question 1? Explain
how you determined this.
Accept/Reject =
3. Assume each pot represents a sample that you found in a
group of wildflowers. Based upon the diversity of flowers
present in each pot; would you say that the ecosystem is
healthy? Why or why not?
Answer =
4. How does biodiversity contribute to the overall health of an
ecosystem? Provide specific examples and utilize at least one
scholarly resource to back your answer.
Answer =
References
Any sources utilized should be listed here.
© eScience Labs, 2015

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  • 1. GENERAL EDUCATION COMMON ASSIGNMENT I. TITLE & NUMBER OF THE COURSE: GOVT 2305 or 2306 II. TITLE OF THE ASSIGNMENT: Course Level Assessment - Content Learning & General Education Outcomes - Government 2305 & 2306 III. GENERAL EDUCATION CORE OBJECTIVES TO BE ASSESSED WITH THIS: A. Communication Skills B. Critical Thinking C. Social Responsibility D. Personal Responsibility IV. DESCRIPTION OF ASSIGNMENT Objectives of the Writing Assignment · Learn about a current political issue or problem of political consequence. · Develop your Internet and library research skills. · Evaluate your topic from the perspective of different cultures and/or political viewpoints as well as your own. · Improve personal and social responsibility. · Improve communication and critical thinking skills. Paper Criteria · Topic selection— A current governmental or political (not historical) issue or problem of importance · Content— address the various sides of the issue with the student drawing personal conclusions based on their own analysis of the issue, and demonstrating an understanding of its complexities. Compare and contrast the different viewpoints of sources used on your topic taking into account the limits of a position. Present an informed evaluation of the evidence and different viewpoint surrounding the topic. · Paper length - your paper should be at minimum (no
  • 2. maximum) of 750 words long (not counting headings). The paper should be typewritten and double-spaced, and based on information from a minimum of three sources (no maximum). · Writing style—with few or no typographical errors, misspelled words, and grammatical errors. Use proper paragraphing and improve your writing style by revising the paper as many times as necessary. · Citations—anytime you borrow someone’s ideas, paraphrase or quote them, cite all sources using an appropriate method approved by the instructor. Analyze and question your sources assumptions. · A bibliography or “work cited” page also using an appropriate method approved by the instructor. Environmental Science Table of Contents 37 Lab 3 Biodiversity
  • 3. Biodiversity Concepts to Explore • Biodiversity • Species diversity • Ecosystem diversity • Genetic diversity • Natural selection • Extinction Introduction Biodiversity, short for biological diversity, includes the genetic variation between all organisms, species, and populations, and all of their complex communities and ecosystems. It also reflects to the interrelatedness of genes, species, and ecosystems and their interactions with the
  • 4. environment. Biodiversity is not evenly distrib- uted across the globe; rather, it varies greatly and even varies within regions. It is partially ruled by climate, whereas tropical regions can support more species than a polar climate. In whole, biodiversity represents variation within three levels: • Species diversity • Ecosystem diversity • Genetic diversity It should be noted that diversity at one of these levels may not correspond with diversity within other levels. The degree of biodiversity, and thus the health of an ecosystem, is im- pacted when any part of that ecosystem becomes endan- gered or extinct. The term species refers to a group of similar organisms that reproduce among themselves. Species diversity refers to the variation within and between populations of species, as well as between different species. Sexual reproduction criti- cally contributes to the variation within species. For exam- ple, a pea plant that is cross-fertilized with another pea plant can produce offspring with four different looks! This genetic mixing creates the diversity seen today.
  • 5. Figure 1: There are more than 32,000 species of fish – more than any other vertebrate! 39 Biodiversity Ecosystem diversity examines the different habitats, biological communities, and ecological processes in the biosphere, as well as variation within an individual ecosystem. The differences in rainforests and deserts represent the variation between ecosystems. The physical characteristics that determine ecosystem diversity are complex, and include biotic and abiotic factors. ? Did You Know...
  • 6. A present day example of natural selection can be seen in the cray- fish population. The British crayfish are crustaceans that live in rivers in England. The American crayfish was introduced to the same bodies of water that were already populat- ed by the British crayfish. The American crayfish are larger, more aggressive and carry an infection that kills British crayfish but to which they are immune. As a re- sult, the British crayfish are de- creasing in number and are ex- pected to become extinct in Britain within the next 50 years. Thus, the American crayfish have a genetic variation that gives them an ad- vantage over the British crayfish to survive and reproduce. The variation of genes within individual organisms is genetic diversity. This can be measured within a species as well as between species. It plays an important role in survival and adaptability of organisms to changing environments.
  • 7. Diversity is also influenced by natural selection, the key mechanism of evolution. The process of natural selection describes competition be- tween individual species for resources such as food and space (habitat). Genetic variations among species provide an advantage over other species if those variations result in an ability to survive and repro- duce more effectively. Evidence that supports the theory of natural selection include the fossil record of change in earlier species, the chemical and anatomical simi- larities of related life forms, the geographical distribution of related spe- cies, and the recorded genetic changes in living organisms over many generations. Take for example, homologous structures among different species, such as the wing of a bird and the forearm of a human. These structures provide evidence that embryologically similar structures can give rise to different functions based on the needs of the organism. Note that natural selection does not try to explain the origin of life but rather the later evolution of organisms over time. Biodiversity is important to the process of evolution because it
  • 8. provides the framework on top of which natural selection can occur. As dis- cussed above, natural selection determines the genetic fitness, an or- ganism's genetic contribution to the next generation, of an organism. Natural selection occurs by selecting one trait as "more advantageous" in a certain environment than anoth- er. The root of this selection is biodiversity. Species extinction is not new; species have been evolving and dying out since life began. Now, however, species extinction is occurring at an alarming rate, almost entirely as a direct result of human activities. Sci- 40 Biodiversity entists recognize five major mass extinctions in the Earth’s history. The extinctions are measured in terms of large groups of related species, called families. The five mass extinction episodes occurred because of major changes in the prevailing ecological conditions brought about by climate change, cataclysmic volcanic erup- tions, or collisions with giant meteors. The sixth mass extinction appears to be in progress now, and the pri- mary cause is environmental change brought about by human activities. Some examples of species on the
  • 9. “endangered” list are the ivory billed woodpecker, amur leopard, javan rhinoceros, northern great whale, mountain gorilla, and the leatherback sea turtle. Figure 2: The amur leopard is at risk of extinction. Loss of an individual species can have various effects on the remaining species in an ecosystem. These ef- fects depend upon the how important the species is in the ecosystem. Individual species and ecosystems have evolved over millions of years into a complex interdependence. If you remove enough of the key spe- cies on which the framework is based, then the whole ecosystem may be in danger of collapsing. Regardless of a species’ place in the ecosystem, it is important for humans to take care of the world around us. As peo- ple become more aware of how their actions impact all living things they can make adjustments in an effort to preserve life on all levels. 41 Biodiversity There are many activities that humans take part in that impact the environment and biodiversity. The exhaust from automobile and aircraft travel as well as smoke stacks from industrial plants are the leading causes of air
  • 10. pollution, which can have harmful effects on natural resources and organisms. Two other important factors which can have an effect on biodiversity are overpopulation and affluence. Overpopulation means that there are more people than resources to meet their needs. As people become more affluent there is an increase in per capita resource utilization. All of these factors contribute to overharvesting, habitat degradation, and in- creased pollution which threaten biodiversity. 42 Biodiversity Demonstration 1: Interdependence of Species In this lab, you will use the information provided above to demonstrate how the presence or absence of one species can affect the others in an ecosystem. Follow the procedure below to complete Demonstration 1 on the interdependence of species. Materials 5 different colored beads: White bead represents lichen
  • 11. Orange bead represents trees Red bead represents flowers Yellow bead represents bees Blue bead represents humans Lichens Lichens play a part in the creation of soils from which plants can obtain nutrients. Like all living organisms, lichens need nutrients and energy to grow. Nutrients may be obtained from the air (including dust), water, and from the substrate organisms grows on. They obtain energy through photosynthesis, which is the role of the algal partner. They may also be incidentally fertilized by bird and insect dung. Trees Most trees, flowers and plants depend on soil for food (nutrients). Fruiting trees depend on bees as one means of pollination. Flowers Forest flowers and plants depend on trees for shade and wind
  • 12. protection as well as soils for nutri- ents. Bees Bees depend on flowering plants and trees for food. Humans Humans depend upon bees for honey and more importantly for fruit from trees they pollinate. 43 Biodiversity Procedure 1. Download the Week 3 Lab Reporting Form from the course instructions. As you conduct the demonstra- tion and the experiment, record any hypotheses, observations, and data on that form. 2. Place all of the beads in a bag. 3. Randomly choose 4 beads from the bag.
  • 13. 4. Identify each bead by the color code in the materials box. 5. Record which species is missing in Table 1 on the Week 3 Lab Reporting Form. 6. Repeat this process 3 more times (or until 3 different beads are taken out of the diagram). Be sure to rec- ord which species is missing from each round in Table 1 and answer the Post-Lab questions on the Week 3 Lab Reporting Form. Experiment 1: Diversity of Plants Variations in growing conditions, climate, and numerous other factors can alter the biodiversity of an ecosys- tem. As such, biodiversity is often utilized as an indicator of ecosystem health. In this experiment, you will grow a sample of seeds under two different conditions. Follow the procedure below to complete Experiment 1 on the diversity of plants. Materials
  • 14. Seed mixture (zinnia, marigold, morning glory, cosmos, and ryegrass) Potting soil (2) 5.5 x 3.5 in Peat pots 10 mL Graduated cylinder *Water *You must provide Procedure 1. Read through the Experiment 1 procedure and then record your hypothesis on the biodiversity of seeds grown under two separate conditions on the Week 3 Lab Reporting Form. 2. Fill your pots loosely with soil until the soil is approximately
  • 15. 1 inch from the top. 3. Pour approximately 40 mL of tap water into your pots (less if the soil becomes very wet). 44 Biodiversity 4. Lightly scatter your seeds on top of the soil in each container. This should be a random assignment of seeds to each pot. 5. Press each seed down about ½ inch into the soil. 6. Place one pot in a sunny, indoor location (on a window sill that receives sunlight) and the second pot in a shaded, indoor location (away from all windows, this pot should not be placed in the dark, just away from direct sunlight). 7. Observe and water your seeds every day until you see them grow. These seeds will germinate quickly (3
  • 16. - 7 days). 8. Complete Table 2 on the Week 3 Lab Reporting Form after approximately 1 - 2 weeks (or when you see a reasonable amount of plant growth in the peat pot) and answer all Post-Lab Questions on the Week 3 Lab Reporting Form. Table 3 (provided on the following page) provides pictures of the germinated seeds to help you determine when you should begin entering data, and what each plant looks like. 45 Weather and Climate Change Table 3: Picture and Description of Seedlings Grown from Seed Mixture Species Observed Picture Description Zinnia Short stems with dark green, rounded leaves Marigold
  • 17. Stems are shorter than cosmos with long skinny leaves (but wid- er than the cosmos leaves) with rounded tips Morning Glory Tall stems with elephant ear shaped leaves Cosmos Tall stems with long, pointed leaflets; a lighter green leaf com- pared to the marigold Ryegrass Long, skinny strands of green grass 46 Appendix Good Lab Techniques
  • 18. Good Lab Techniques Good Laboratory Techniques Science labs, whether at universities or in your home, are places of adventure and discovery. One of the first things scientists learn is how exciting experiments can be. However, they must also realize science can be dangerous without some instruction on good laboratory practices. • Read the protocol thoroughly before starting any new experiment. You should be familiar with the action required every step of the way. • Keep all work spaces free from clutter and dirty dishes. • Read the labels on all chemicals, and note the chemical safety rating on each container. Read all Material Safety Data Sheets (provided on www.eScienceLabs.com).
  • 19. • Thoroughly rinse lab ware (test tubes, beakers, etc.) between experi- ments. To do so, wash with a soap and hot water solution using a bottle brush to scrub. Rinse completely at least four times. Let air dry • Use a new pipet for each chemical dispensed. • Wipe up any chemical spills immediately. Check MSDSs for special handling instructions (provided on www.eScienceLabs.com). • Use test tube caps or stoppers to cover test tubes when shaking or mixing – not your finger! A B C Figure 1: A underpad will prevent any spilled liquids from contaminating the sur- face you work on. Figure 2: Special measuring tools in make experimentation easier and more accu- rate in the lab. A shows a beaker, B graduated cylinders, and C test
  • 20. tubes in a test tube rack. 67 Good Lab Techniques • When preparing a solution, refer to a protocol for any specific instructions on preparation. Weigh out the desired amount of chemicals, and transfer to a beaker or graduated cylinder. Add LESS than the required amount of water. Swirl or stir to dissolve the chemical (you can also pour the solution back and forth between two test tubes), and once dissolved, trans- fer to a graduated cylinder and add the required amount of liquid to achieve the final volume. • A molar solution is one in which one liter (1L) of solution con- tains the number of grams equal to its molecular weight. For example: 1M = 110 g CaCl x 110 g CaCl/mol CaCl (The formula weight of CaCl is 110 g/mol)
  • 21. Figure 3: Disposable pipettes aid in ac- curate measuring of small volumes of liquids. It is important to use a new pi- pette for each chemical to avoid con- tamination. • A percent solution can be prepared by percentage of weight of chemical to 100ml of solvent (w/v) , or volume of chemical in 100ml of solvent (v/v). For example: 20 g NaCl + 80 mL H2O = 20% w/v NaCl solution • Concentrated solutions, such as 10X, or ten times the normal strength, are diluted such that the final concentration of the solution is 1X. For example: To make a 100 mL solution of 1X TBE from a 10X solution: 10 mL 10X TBE + 90 mL water = 100ml 1X TBE • Always read the MSDS before disposing of a chemical to insure it does not require extra measures. (provided on www.eScienceLabs.com)
  • 22. • Avoid prolonged exposure of chemicals to direct sunlight and extreme temperatures. Immediately se- cure the lid of a chemical after use. • Prepare a dilution using the following equation: c1v1 = c2v2 Where c1 is the concentration of the original solution, v1 is the volume of the original solution, and c2 and v2 are the corresponding concentration and volume of the final solution. Since you know c1, 68 Good Lab Techniques c2, and v2, you solve for v1 to figure out how much of the original solution is needed to make a cer- tain volume of a diluted concentration. • If you are ever required to smell a chemical, always waft a gas toward you, as shown in the figure below.. This means to wave your hand over the chemical towards you. Never directly smell a chemical. Never smell a gas that is toxic or otherwise
  • 23. dangerous. • Use only the chemicals needed for the activity. • Keep lids closed when a chemical is not being used. • When diluting an acid, always slowly pour the acid into the water. Never pour water into an acid, as this could cause both splashing and/or an explosion. • Never return excess chemical back to the original bottle. This can contaminate the chemical sup- ply. • Be careful not to interchange lids between different chemical bottles. • When pouring a chemical, always hold the lid of the chemical bottle between your fingers. Never lay the lid down on a surface. This can contaminate the chemical supply. • When using knives or blades, always cut away from yourself. 69
  • 24. © 2012 eScience Labs, LLC - All rights reserved 68 Lab 3Concepts to ExploreIntroductionDemonstration 1: Interdependence of SpeciesLichensMaterialsProcedureAppendixA B C© 2012 eScience Labs, LLC - All rights reserved Lab 3 – Biodiversity Demonstration 1: Interdependence of Species Table 1: Interdependence of Species Results Round Species Missing (Bead Color and Name) 1 2 3 4 POST LAB QUESTIONS 1. Explain how the ecosystem was affected by the missing species for each round of the demonstration.
  • 25. a. Round 1 = b. Round 2 = c. Round 3 = d. Round 4 = 2. Provide one action we as humans engage in that leads to the extinction of each of these components. Action affecting Lichens = Action affecting Trees = Action affecting Flowers = Action affecting Bees = 3. Provide three specific actions that humans can take to minimize our impact on the ecosystem and ensure the survival of lichens, trees, flowers, and bees. Action 1 = Action 2 = Action 3 = Experiment 1: Diversity of Plants Table 2: Number of Each Plant Species Present in Pot 1 and Pot 2 Species Observed Number in Pot 1(sunlight) Number in Pot 2(shade) Zinnia Marigold
  • 26. Morning Glory Cosmos Ryegrass Total Number of Species in Pot: POST LAB QUESTIONS 1. Develop a hypothesis on which pot you believe will contain the highest biodiversity. Hypothesis = 2. Based on the results of your experiment, would you reject or accept the hypothesis that you produced in question 1? Explain how you determined this. Accept/Reject = 3. Assume each pot represents a sample that you found in a group of wildflowers. Based upon the diversity of flowers present in each pot; would you say that the ecosystem is healthy? Why or why not? Answer = 4. How does biodiversity contribute to the overall health of an ecosystem? Provide specific examples and utilize at least one scholarly resource to back your answer. Answer = References Any sources utilized should be listed here.