2. F. Nemati et al. / Sensors and Actuators B 255 (2018) 2078–2085 2079
the intrinsic defects of those QDs limit their widespread applica-
tion, such as complex synthesis procedures, heavy metals potential
toxicity and environmental hazards [24]. Graphene quantum dots
(GQDs) are generally defined as zero-dimensional carbon-based
material that are typically single or few-layer graphenes with lat-
eral dimensions of less than 100 nm [25,26]. Owing to the novel
properties, such as remarkable conductivity, high edge-to-area
ratio, stable luminescence, good biocompatibility, and low toxicity,
graphene quantum dots have recently emerged as promising alter-
natives to traditional quantum dots in some aspects [27]. However,
the photoluminescence quantum yield of the pristine GQDs is usu-
ally low and much lower than semiconductor quantum dots [28].
Recently demonstrated that high quantum yields of GQDs quantum
yield can be observed after either functionalization or doping [29].
Among foreign atoms, Nitrogen has been widely used for chem-
ical doping in carbon-based nanomaterials by virtue of it having
match able atomic size and five valence electrons for bonding with
carbon atoms [30,31]. The optimization in analytical chemistry is
generally carried out with a traditional single variable approach
(SVA) [32]. Its major disadvantage is that it ignores the combination
influence among the parameters studied. Due to above disadvan-
tage, multivariate statistic methods have been applied to optimize
experimental parameters. Among the most relevant multivari-
ate techniques, response surface methodology (RSM) has shown
considerable application in analytical optimization [33]. Response
surface methodology, a collection of mathematical and statistical
techniques, is a very useful tool for determining the effect of opera-
tional variables for process development and optimization [34,35].
The most frequent and efficient methods used in response surface
methodology is central composite design (CCD) [36]. The aim of
this work was to develop a simple, fast and non-toxic method, of
maximum possible sensitivity, to detection of thiamine in phar-
maceuticals and foodstuff samples. For this, we have synthesized
water-soluble Arg-GQDs by using citric acid and Arginine (Arg) as
carbon and nitrogen sources respectively. Based on the excellent
photoluminescent of the Arg–GQDs, a simple, potent, fast, non-
toxic fluorescent probe was designed. In this paper, the influence
of time, temperature and pH on fluorescence sensing was investi-
gated using response surface methodology (RSM) based on central
composite design (CCD). The range of linearity, the stability, the
response to interferents and the detection limit (LOD) of the sensor
have been evaluated. The optical sensor based on the Arg-GQDs has
been successfully applied in sensitive detection of thiamine in real
spiked samples.
2. Experimental section
2.1. Chemicals and materials
Citric acid (+99%), Arginine were purchased from Sigma–Aldrich
Co. Thiamine hydrochloride (98%) was purchased from Alfa
Aesar. All the other chemicals used were of analytical reagents
grade. Stock standard solution of thiamine hydrochloride
(1.0 × 10−3 mol L−1) was prepared in water and stable for at
least 1 month when kept refrigerated. Working solutions of lower
concentrations were prepared daily from the above stock solution
as required. All chemicals used were of analytical grade or of the
highest purity available. All solutions were prepared with doubly
deionized water (DDW).
2.2. Apparatus
UV–vis measurements were carried out on a PG Instruments
T80+ UV/Vis spectrophotometer and the spectra were collected
from 200 nm to 700 nm. All fluorescence measurements were
carried out by a Perkin-Elmer LS50 luminescence spectrometer.
Fourier transform infrared (FT-IR) spectrum was recorded on a
Shimadzu-8400S spectrometer in the range of 400–4000 cm−1 at
room temperature using KBr pellets. Energy-dispersive X-ray (EDX)
spectra were obtained using the Philips XL30 scanning electron
microscope (SEM). The morphologies of Arg-GQDs samples were
characterized by transmission electron microscopy (TEM, Philips,
CM30, 300 Kv). The pH was adjusted and measured using pH meter
Metrohm. The X-ray diffraction patterns (XRD) were recorded on
a Philips diffractometer (model X’Pert MPD). Raman spectrum was
recorded using an Almega Thermo Nicolet Dispersive Raman Spec-
trometer with a 532 nm laser.
2.3. Synthesis of functionalized graphene quantum dots
In a typical synthesis, 0.21 g of citric acid and 0.10 g of Arginine
were mixed with 5 mL water, and stirred to form a clear solu-
tion. The solution was then transferred into a 20 mL Teflon-lined
stainless autoclave. The sealed autoclave was heated constantly
at 160 ◦C for 4 h. The obtained mixture was filtered with a mem-
brane (0.22 m) to remove the large dots. Finally, the as prepared
Arg-GQDs were stored at 4 ◦C for further experiments.
2.4. General procedure
For thiamine detection, 25 L of 40 mg mL−1 Arg-GQDs, 1 mL pH
6 phosphate buffer and 40 L (0.01 M) of Ag+ solution were added
in a quartz cuvette and the mixture was stirred thoroughly. Then,
the mixture was finally diluted to 2 mL with ultrapure water. Next,
different amounts of thiamine added to the above mixed solution.
Finally, the fluorescence intensity of the mixture was recorded at
excitation wavelength of 350 nm, and the band-slits of both exci-
tation and emission were set as 10.0 and 15.0 nm, respectively.
2.5. Quantum yield measurements
The quantum yield of the as-synthesized GQDs were determined
by using quinine sulfate as the fluorescence standard and was cal-
culated according to the following Eq. (1):
Yu = Yst
Iu
Ist
Ast
Au
n2
u
n2
st
(1)
Where Y is the quantum yield, I is the measured integrated emission
intensity, n is the refractive index, and A is the extinction. The sub-
script “st” refers to the standard and “u” for sample with unknown
QY.
2.6. Analysis of thiamine in real samples
Fluorescent detection of thiamine in food samples was per-
formed through the following steps: 1.0 g of samples that was
purchased from a local supermarket was grounded and mixed with
0.1 mol L−1 HCl solution. After integrating for 15 min, the mixture
was centrifuged for 15 min at 5000 rpm, and the supernatant was
filtered through a 0.45 mm cellulose acetate filter and diluted up to
10 mL with ultra-pure water. The pharmaceutical sample (vitamin
B1 tablet from Jalinous Pharmaceutical Co., Iran) was obtained from
the local drug store. For analysis, a piece of vitamin B1 tablet was
powdered in a mortar and dissolved in ultrapure water. The mixture
was sonicated for 10 min for fully dissolution. The insoluble com-
ponents were removed by filtration. After that, the solution was
transferred into 25 mL volumetric flask and stored in dark at 4 ◦C.
Standard addition method was used for determination of thiamine
in the real samples.
3. 2080 F. Nemati et al. / Sensors and Actuators B 255 (2018) 2078–2085
Fig. 1. (a) TEM image of the Arg-GQDs (inset: size distribution of Arg-GQDs) (b) EDX spectrum of the Arg-GQDs (c) Raman spectra of as-prepared Arg-GQDs (d) XRD patterns
of the Arg-GQDs.
Table 1
Experimental factors and levels in CCD.
Levels
Independent variable -␣ -1(low) 0 (center) +1(High) +␣
(x1) pH 4 5 6 7 8
(x2) time(min) 5 10 15 20 25
(x3) temperature (
◦
C) 25 30 35 40 45
2.7. Optimization of factors affecting the fluorescence sensing of
thiamine using RSM approach
Central composite design was applied to investigate the effects
of the three operational factors on the response function. The
studied parameters were selected based on the preliminary experi-
ments and prior knowledge from literature. The high and low levels
defined for three independent variables are shown in Table 1. After
running the 20 trials, a second order polynomial model for predict-
ing the optimal point was explained by the following Eq. (2):
Y = ˇ0 +
k
i=1
ˇixi +
k
i=1
k
j=i+1
ˇijxixj +
k
i=1
ˇiix2
i
(2)
In the above equation, Y is the predicted response; xi and xj are
independent factors and ˇ0 is the intercept. ˇi and ˇii are the lin-
ear and quadratic coefficients, respectively. While ˇij represents
the interaction coefficients. Design Expert software version 7 was
employed to analyze the data and the design of the experiment.
Table 2
The design matrix for four variables with enhancement ratio (F- F0)/F0 as response
(R).
Runs Block x1 x2 x3 (F − F0)/F0
1 1 6 15 35 2.73
2 1 7 10 30 2.68
3 1 5 10 30 2.54
4 1 5 20 40 2.26
5 1 5 20 30 2.10
6 1 7 20 30 2.48
7 1 7 20 40 2.43
8 1 7 10 40 2.28
9 1 5 10 40 2.09
10 1 6 15 35 2.60
11 2 6 15 35 2.55
12 2 6 15 35 2.49
13 2 8 15 35 2.02
14 2 6 15 45 2.39
15 2 6 15 35 2.53
16 2 4 15 35 1.37
17 2 6 15 35 2.70
18 2 6 25 35 2.51
19 2 6 5 35 2.37
20 2 6 15 25 2.75
The design matrix and enhancement ratio (F − F0)/F0 as response
are presented in Table 2.
4. F. Nemati et al. / Sensors and Actuators B 255 (2018) 2078–2085 2081
Fig. 2. (a) FT-IR spectrum (b) UV–vis absorption (c) fluorescence excitation and
emission spectra of Arg-GQDs.
3. Results and discussion
3.1. Characterization of GQDs
The Arg-GQDs were synthesized by the hydrothermal approach
described in the Experimental Section. TEM images gave the first
confirmation that nano-sized pieces formed during the synthesis
of NGQDs. Fig. 1a shows the TEM image of N-GQDs. Uniformly dis-
persed Arg-GQDs in a narrow size distribution were observed. And
the mean diameter of Arg-GQDs was estimated to be 5.5 nm.
Analysis of elemental composition of the prepared Arg-GQDs
was performed using EDX. The EDX spectrum of the N-GQD showed
the presence of elements O, N, and C (Fig. 1b).Raman spectrum of
the of Arg-GQD is shown in Fig. 1c. There are two closely peaks
at around 1600 cm−1 (G band) and 1400 cm−1(D band), respec-
tively, which were assigned to in-plane vibrations of crystalline
graphite and disordered structures of the sp2 domains. For more
information about the structure, X-ray diffraction (XRD) analysis
was performed. There is a diffraction peak at range of 21.8–24.4◦
on the XRD pattern, which attributed to graphitic structure [37,38].
Fig. 2a showed the FT-IR spectrum of N-CQDs in the wavelength
range of 500–4000 cm−1. The absorption band at 3420 cm−1 was
assigned to O H stretching vibration. The characteristic absorption
peaks for C = C at 1571 cm−1 and C C at 1234 cm−1 were observed,
indicating the existence of delocalized -electrons in Arg-GQDs
molecules. The absorption peaks around 3172 cm−1 and 1451 cm−1
were belonged to of N H and C N stretching vibration, respec-
tively [39]. This result further confirmed the doping of N in the
as-prepared sample. Moreover, three absorption peaks appeared
at 1451 cm−1and 1109 cm−1, corresponding to the stretching vibra-
tions of COO and C O groups, respectively. These functional groups
could stabilize Arg-GQDs in aqueous media. The as-prepared Arg-
GQDs solution remains homogeneous even after 2 months at room
temperature without any perceptible changes (e.g., aggregation or
color change), which could be further characterized by the almost
unchanged absorption and PL spectra (Fig. S1). To further explore
the optical properties of the N-GQDs, UV–vis absorption and FL
spectra were recorded. As shown in Fig. 2b, UV–vis absorption spec-
trum of the GQD shows a typical absorption peak at ca. 210 nm
was observed, which is assigned to the –* transition of aro-
matic C C sp2 domains. Another strong absorption peak at 340 nm
can be regarded as the n–* transition of the C O bone, which
can result in strong fluorescence [40]. The GQD solution emitted
strong blue FL under excitation at 365 nm by a UV lamp (inset of
Fig. 2a). However, it was light yellow, transparent and clear under
daylight (inset of Fig. 2b). As shown in Fig. 2c, the Arg-GQDs exhib-
ited a maximum emission at 448 nm with an excitation wavelength
of 350 nm. Compared to the non-doped GQDs, the present Arg-
GQDs show blue-shifted emission, which could be ascribed to the
strong electron affinity of the doped nitrogen [41]. It should be
noted that the excitation peak is very close to the absorption peak,
suggesting the high-efficient emitting absorption of the Arg-GQDs.
The quantum yield of the Arg-GQDs was calculated to be 28.3%.
The photostability of as-synthesized Arg-GQDs under UV irradia-
tion was studied. As shown in Fig. S2, the fluorescence intensity of
Arg-GQDs remained constant within the studied range, indicating
excellent photo-stability of the as-prepared Arg-GQDs. The effects
of ion strength on the fluorescence of Arg-GQDs was also inves-
tigated in the presence of different concentrations of NaCl. The
signal intensity measured of the Arg-GQDs. When concentrations
of NaCl is 0.1–2 M, the measured fluorescence was nearly constant,
indicating the high stability of Arg-GQDs under high salt medium.
3.2. Optimization of sensing conditions
Based on CCD experiment, the effects of detection parame-
ters; incubation time, pH value and temperature were evaluated.
According to experimental data, using software the second-order
polynomial was used to describe the response variable (F − F0/F0)
and interaction among the variables as follows Eq. (3):
F − F0/F0 = +2.60 + 0.14x1 − 0.003750x2
− 0.090x3 + 0.027x1x2 − 0.020x1x3
+ 0.12x2x3 − 0.22x1
2
− 0.036x2
2
0.003542x3
2
(3)
Where x1, x2 and x3 are pH, time and temperature, respectively. In
order to test the validity and adequacy of the model and the effect
of operational variables and their interactions, ANOVA was per-
formed. The ANOVA is a collection of statistical models, and their
associated procedures, in which the observed variance in a particu-
lar variable is partitioned into components attributable to different
sources of variation. It provides a statistical test of whether or not
the means of several groups are all equal [42]. The reliability of
the suggested model was assessed by the coefficient of determina-
tion (R2 and adjusted-R2). An R2 of 0.9532 and an adjusted-R2 of
0.9065 indicated excellent correlation between experimental val-
ues and fitted model, and showed a high- efficiency designed model
for prediction of response. Based on ANOVA data, the amounts of
F-value (20.38) and p-value (less than 0.0001) implies that defined
model is significant. The “lack-of-fit (LOF) P-value” of this model
(0.6243) is in significant because of its amount is more than 0.05
5. 2082 F. Nemati et al. / Sensors and Actuators B 255 (2018) 2078–2085
Fig. 3. The predicted response vs. the observed response.
Fig. 4. A plot of the internally studentized residuals vs. the predicted response.
critical value; thus, the non-significant lack-of-fit confirm the ade-
quacy of a model fit. Fig. 3 indicates the predicted responses versus
observed data. Most residuals were proximity close to the straight
line, which indicates a good relationship between actual data and
fitted model as a cause of large R2 values.
The plot of the residuals versus the predicted data (Fig. 4) con-
firms that the points were distributed randomly. In next step, to
observe the individual and interactive effect of the variables on
enhancement ratio (F − F0)/F0, 3-D surface plots and contour plots
were used. The responses plotted relative to the two significant
variables, while the other factor is fixed at its central level [43].
The goal of the response surface is to find the optimum values
of the factors, such that the response is maximized [44]. Fig. 5a
depicts the effect of pH, time and their mutual interaction on the
response. Thiamine has been reported to get oxidized at alkaline
medium to generate the thiochrome [45]. Hence, the pH effect was
studied in the 4–8 range. Result show that by increasing the pH of
solution, the enrichment ratio increases. This is related to protona-
tion of the carbonyl groups and hydroxyl groups on the surface of
Arg-GQDs that occur at the acidic pH. In other hand, at high con-
centration of H+ ions, the formation of hydrogen bond between
oxygen-containing functional groups on the surface of GQDs lead
to the aggregation of GQDs [46]. A pH of 6 was selected for the fur-
ther thiamine sensing. As can be seen from Fig. 5a–c, the higher
temperature leads to a decrease in the response surface and had an
inverse effect on enhancement ratio. The fluorescence signal of a
graphene quantum dot is very sensitive to temperature. The quan-
tum efficiency includes radiative and non-radiative decay of excited
states. The enhance in non-radiative decay rate at higher temper-
ature leads to decrease in quantum efficiency and corresponding
Fig. 5. Response using the central composite design obtained by plotting: (a) time
vs. pH, (b) temperature vs. pH, (c) time vs. temperature.
fluorescence intensity [47]. Effects of incubation time and temper-
ature on the enhancement ratio are shown in Fig. 5c. Incubation
time and temperature both had inverse effect on the enhancement
ratio, while the interaction between incubation time and temper-
ature was negatively correlated with response. The enhancement
ratio can be adjusted by changing the time because it decreases the
temperature. Therefore, the low temperature leads to an increase
in the initial intensity. The optimum predicted point of maximum
enhancement ratio obtained by Design-Expert software is about
2.77, and the corresponding optimal parameters of adsorption pro-
cess are listed as below: time 10 min, temperature 30 ◦C, pH 6.
3.3. Possible mechanism
Upon addition of Ag+ ion to the dispersion of the Arg- GQDs, a
decrease in fluorescence intensity of Arg-GQDs is observed (Fig. 6).
The nature of quenching was attributed to the coordination of Ag+
and the functional groups of Arg-GQDs. According to HSAB (hard
and soft acids and bases) principle, the soft acids and soft bases
tend to have a strong interaction [48]. Thus, Ag+ ions (soft acids)
could have a high affinity with nitrogen atoms in Arg-GQDs surface
(soft bases) and form conjugation of the Arg-GQDs- Ag+ complex
[49]. Thus, the fluorescence of Arg-GQDs was quenched by the addi-
tion of Ag+ ions. The interaction between Arg-GQDs and Ag+ was
6. F. Nemati et al. / Sensors and Actuators B 255 (2018) 2078–2085 2083
Fig. 6. Fluorescence responses Arg-GQDs in the presence of several of Ag+
.
studied by FT-IR spectra (Fig. S4). When Ag+ is present in Arg-GQDs
solution, the peak position of N H stretching vibration band moves
from 3172 to 3170 cm−1 and the peak position of COO− stretch-
ing vibration band moves from 1451 to 1454 cm−1, together with
the decrease in the peak intensities of these two bands. The peak
intensities of 3420 also decreased, confirming the binding interac-
tion between Arg-GQDs and Ag+. These results indicate that Ag+
interacted with functional groups present on the obtained Arg-
GQDs. According to the experimental result, we considered that
thiamine with thiazole ring would show strong binding ability
with Ag+ through an Ag S bond [50]. Obviously, the fluorescence
intensity of the quenched N-GQD recovered with increasing con-
centration of thiamine in the system. This enhancement could be
a result of interaction between Ag+ and the thiamine. This result
was further confirmed by the Zeta potential measurement of Arg-
GQDs at different condition (Fig. S5). A significant shift of the zeta
potential from −28.1 mV of free N-GQDs to −12.6 in the presence of
Ag+ shows the interaction between Arg-GQDs and Ag+ ions. On the
other hand, the increase of the zeta potential to −22.3 mV resulted
from the reaction of the Ag+ with thiamine, which successfully
confirms the remove of Ag+ ions from the surface of the N-GQDs.
3.4. Analytical characteristics
To demonstrate the sensing performance of this proposed assay
for thiamine detection, we evaluated the response of this sensor
by adding varying concentrations of thiamine under experimen-
tal optimal conditions (Fig. 7a). Fig. 7b presents the relationship of
the enhancement ratio (F − F0)/F0 (F0 and F are the fluorescence
intensities of Arg-GQDs at 448 nm in the absence and presence
of thiamine, respectively) with the concentration of thiamine. The
inset of Fig. 7b shows that the (F − F0)/F0 has a good linear correla-
tion with the concentration of thiamine in the range of 0.1–8 M,
and the linear regression equation is y = 0.4505 + 0.0556C, where C
is the concentration of thiamine (M). The corresponding regres-
sion coefficient was 0.995 and the detection limit of this method
for thiamine was 53 nM (S/N = 3). Furthermore, the analytical per-
formance of the proposed sensing system for thiamine detection is
comparable to those reported (Table 4).
3.5. Selectivity
In order to understand the selectivity of the reported assay
and interference to thiamine detection, the effect of some com-
pounds abundant in the selected real samples on the fluorescence
behavior of probe was examined. Under the optimal conditions, the
developed method was used to including pyridoxine (vitamin B6),
cyanocobalymine (vitamin B12), ascorbic acid (vitamin C), niacin
Fig. 7. (a) Fluorescence response of Arg-GQDs containing Ag +
to various con-
centrations of thiamine. (b) Plot of the enhancement ratio (F − F0)/F0 vs thiamine
concentration. The Inset shows a linear relationship in the concentration range from
0.1 to 8 M.
Table 3
Analytical results of thiamine in food samples and vitamin B1 tablet (n = 3).
Sample Added (M) Found (M) Recovery (%, n = 3) RSD (%, n = 3)
Green pea 0.3 0.28 93 2.9
2 1.95 97 3.1
Sunflower 0.3 0.31 103 1.4
2 2.05 102 3.7
Wheat flour 0.3 0.29 96 4.5
2 2.11 105 2.3
VB1 tablet 0 2.34 – 4.8
0.3 2.61 90 3.6
2 4.41 103.5 2.8
(vitamin B3), folic acid (vitamin B9), 100-fold glucose, sucrose, fruc-
tose, lactose, cellulose and1000-fold Na+, K+, Ca2+ and Mg2+. As
shown in Fig. 8a, various interference showed a negligible influence
on the fluorescence intensity of the quenched Arg-GQDs, except for
the fluorescence recovery induced by thiamine. In addition, a com-
petitive experiment for the effect of interferences on the recovery
fluorescence intensity of Arg-GQDs by thiamine was also operated
using the current assay under the same conditions. The result shows
that no change in the fluorescence response of probe towards thi-
amine was observed in the presence of interferences (Fig. 8b). These
results indicate that the as-synthesized Arg-GQDs can be used as
effective fluorescence probe for thiamine detection.
3.6. Application of the fluorescent Arg-GQDs in real sample
To demonstrate the feasibility of the sensing system for detec-
tion in real samples, the fluorescence assay was applied for
thiamine sensing in pharmaceuticals and foodstuff samples by the
7. 2084 F. Nemati et al. / Sensors and Actuators B 255 (2018) 2078–2085
Table 4
Comparison of the present approach with other reported methods for the detection of thiamine.
Method Technique in detail Linearity LOD Ref.
Fluorescence Eu-doped Y2O3 nanoparticles 0–44 M 0.144 M [5]
Fluorescence carbon dots 10–50 M 0.280 M [45]
luminescence CdSe quantum dots 15–120 M 0.207 M [46]
Electrochemical luminescence Rhodamine B 0.3–5 M 0.265 M [47]
HPLC-CLD-UV – 0.07–27 M 0.030 M [48]
Fluorescence N-GQDs 0.1–8 M 0.053 M Present work
Fig. 8. (a) The fluorescence enhancement factors [(F0 − F)/F0] of the Arg-GQDs con-
taining Ag+
with a series of interfering substance and thiamine. (b)The fluorescence
intensities of N-GQDs-Ag+
with thiamine in the presence of interfering substance.
standard addition technique. The samples were diluted to make
sure the thiamine concentration in the real samples were within the
linear range. The obtained results were listed in Table 3. The recov-
ery of thiamine for spiked samples were in the range of 90%–103.5%,
whereas the relative standard deviations range were no more than
4.8%. The obtained results were listed in Table 3. The results indi-
cated that the designed sensor was a reliable method for thiamine
analysis in real samples.
4. Conclusion
In summary, we have developed Arg-GQDs-based fluorescent
sensor for detection of trace amounts of thiamine in pharmaceutical
and foodstuff samples. Arg-GQDs, synthesized via a facile one-step
hydrothermal approach, were demonstrated to be a fluorescent
probe for label-free determination of thiamine with high sensi-
tivity and selectivity. The presence of Ag+ caused the quenching
of fluorescence intensity of Arg-GQDs owing to the coordination
reaction between Ag+ and Arg-GQDs, while subsequent addition
of thiamine restores the fluorescence intensity caused the quench-
ing of fluorescence intensity. The effects of independent variables
were successfully optimized by the assessment of central compos-
ite design. The experimental results which were obtained during
the sensing of thiamine, were found to be fitted with model pre-
diction. The adequacy of regression model was tested by lack-of-fit
(LOF), F values and P values. Furthermore, the proposed method
has been utilized to accurately detect thiamine in practical samples.
Our present work provides the advantage of simplicity, sensitivity,
low cytotoxicity and selectivity.
Acknowledgements
The financial support of this study by Iran University of Science
and Technology, University of Tehran and Iran Nanotechnol-
ogy Initiative Council are gratefully acknowledged. The author
acknowledges financial support from the Iran National Science
Foundation (INSF).
Appendix A. Supplementary data
Supplementary data associated with this article can be found, in
the online version, at http://dx.doi.org/10.1016/j.snb.2017.09.009
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Biographies
Fatemeh Nemati is a Ph.D. student in Analytical Chemistry at Iran University of
Science and Technology, Tehran, Iran.
Rouholah Zare-Dorabei has a PhD in Analytical Chemistry obtained at University
of Tehran in 2010. He is currently Assistant Professor in Analytical Chemistry Iran
University of Science and Technology, Tehran, Iran. His research work has been
mainly focused on the design of new optical sensors.
Morteza Hosseini has a Ph.D. in analytical chemistry obtained at Tarbiat Modares
University in 2005. He is currently an associated professor in nanobiotechnology
group of Faculty of New Sciences and Technologies of University of Tehran. His
research work has been mainly focused on the design of new optical nanosensors
and nanobiosensors.
Mohammad Reza Ganjali has a Ph.D. in analytical chemistry obtained at University
of Tehran in 1997. He is currently a professor of analytical chemistry at University of
Tehran. His research work has been mainly focused on the design of new chemical
sensors.