Label the stages in the Trypanosoma brucei (T. brucei)life cycle in tsetse flies and humans. Solution Starting from the top box 1. Tsetse fly biting human- An infected tsetse fly bites a human and injects T. brucei into the blood stream . 2. In human-T.brucei multiplies in the blood, lymph and spinal fluid of the human via binary fission. 3. Again tsetse fly biting human- An uninflected tsetse fly bites an infected human and ingests T.brucei. 4. In Tsetse fly- T. brucei enters the salivary gland of the fly and multiply 5. In the midgut of tsetse fly- T.brucei multiplies in the midgut of the fly via binary fission.