The document contains graphs showing: 1) Fold induction levels over control for various conditions involving different cell lines and treatments. 2) AXIN-2 qPCR results for different cell lines and treatments. 3) Permeability assay results for RITC 3KD dextran in different cell lines and treatments over time.

1. Fold Induction Over Control
0
1000
2000
3000
4000
C C
on on
tro tro
l+ l
C
1C
C 2b
on
tro
l+
Sc
r
BI BI
O O
+
C
1C
2b
BI
O
+
Sc
r
BI
BI O
O +I
+I C
C G
G
+C
1C
BI
O 2b
+I
C
G
+S
cr

2. Fold Induction Over Control
0
100
200
5000
10000
15000
20000
25000
C C
on on
tro tro
l+ l
C
1C
C 2a
on
tro
l+
Sc
r
BI BI
O O
+
C
1C
2a
BI
O
+
Sc
r
BI
BI O
O +I
+I C
C G
G
+C
1C
BI
O 2a
+I
C
G
+S
cr

3. Fold Induction Over Control
0
100
200
500
1000
1500
2000
2500
3000
3500
4000
C C
on on
tro tro
l+ l
m
C
1C
2
C
2C
2
BI BI
O O
+
m
C
1C
BI
O 2
+
C
2C
2
BI
BI
O O
+I
+I
C C
G G
+m
BI C
O 1C
+I
C 2
G
+C
2C
2

4. AXIN-2 qPCR

5. AXIN-2 qPCR

8. Permeability Assay for RITC 3KD Dextran
1.5×10-05
3×10-06
Pe (cm/s)
2×10-06
1×10-06
0
d5
rt
5
5
nd
nd
se
En
In
bE
bE
-b
e
n-
n-
FP
re
D
D
lF
G
EF
EF
el
3L
5L
C
0.
0.

9. NU-Nuclear Fraction
PN-Post Nuclear Fraction